C-terminal truncated bank vole prion protein (amino acids 90-231)

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  "angular_unit": "1/nm",
  "project": {
    "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
    "publication": {
      "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
      "author_list": "Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G",
      "journal": "Biophysical Journal",
      "doi": "10.1016/j.bpj.2019.12.025",
      "pmid": null,
      "published_date": "2020 Jan"
    },
    "status": "released",
    "submitted_date": "2018-11-13",
    "released_date": "2019-11-25"
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Major prion protein",
          "short_name": null,
          "sequence": "GQGGGTHNQWNKPSKPKTNMKHVAGAAAAGAVVGGLGGYMLGSAMSRPMIHFGNDWEDRYYRENMNRYPNQVYYRPVDQYNNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCVTQYQKESQAYYEGRSS",
          "organism": "Myodes glareolus",
          "uniprot_code": "Q8VHV5",
          "uniprot_range_first": 90,
          "uniprot_range_last": 231,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MANLSYWLLAFFVTTWTDVGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGGTWGQP\nHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQGGGTHNQWNKPSKPKTNMKHVAGAAAAGA\nVVGGLGGYMLGSAMSRPMIHFGNDWEDRYYRENMNRYPNQVYYRPVDQYNNQNNFVHDCV\nNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCVTQYQKESQAYYEGRSSRAVLLFSSP\nPVILLISF",
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          "total_mw": 16.14,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM Ammonium Acetate, 250 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 5.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "C-terminal truncated bank vole prion protein (amino acids 90-231)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "ESRF",
            "address": "Grenoble, France",
            "full_name": "European Synchrotron Radiation Facility",
            "webpage": "http://www.esrf.eu/"
          }
        ],
        "contributor_name": "Gabriele",
        "contributor_surname": "Giachin",
        "orcid": "https://orcid.org/0000-0001-7550-0307"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-02-25",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 1,
    "wavelength": 0.099,
    "sample_detector_distance": 2.864,
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  "experiment_description": "Synchrotron SAXS data from solutions of the C-terminal truncated bank vole prion protein (amino acids 90-231) in 25 mM ammonium acetate, 250 mM NaCl, pH 5.5 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column type, Superdex 75 10/300 GL (GE Healthcare); Flow rate: 0.5 mL/min; Total acquisition time: 50 min; Injection concentration: 10 mg/mL; Injection volume: 250 μL. The data obtained through the sample elution peak (1963-2030 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.",
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  "guinier_point_last": 70,
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  "last_modified": "2020-08-28T15:28:09.828046+02:00",
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