查看完整 summary.json 原文
{
"code": "SASDEX6",
"status": "Published",
"type_of_curve": "Single concentration",
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"project": {
"title": "Structural interpretations of a flexible cold-active AMS8 lipase by combining small-angle X-ray scattering and molecular dynamics simulation (SAXS-MD).",
"publication": {
"title": "Structural interpretations of a flexible cold-active AMS8 lipase by combining small-angle X-ray scattering and molecular dynamics simulation (SAXS-MD).",
"author_list": "Yaacob N, Kamonsutthipaijit N, Soontaranon S, Leow TC, Rahman RNZRA, Ali MSM",
"journal": "Int J Biol Macromol",
"doi": "10.1016/j.ijbiomac.2022.08.145",
"pmid": "36029961",
"published_date": "2022 Aug 24"
},
"status": "released",
"submitted_date": "2018-10-27",
"released_date": "2021-09-20"
},
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},
"name": "Synchrotron Light Research Institute (SLRI)",
"city": "Nakhon Ratchasima",
"country": "Thailand",
"beamline_name": "BL1.3W",
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"type_of_source": "X-ray synchrotron",
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{
"long_name": "LipAMS8",
"short_name": "AMS8",
"sequence": ">tr|E1B2U7|E1B2U7_9PSED LipAMS8 OS=Pseudomonas sp. AMS8 OX=882519 PE=4 SV=1\r\nMGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGS\r\nTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQ\r\nVEVLGKYDGDGKLLEIGIGFRGTSGPRETLITDSIGDLVSDLLAALGPKDYAKNYAGEAF\r\nGTLLKDVAAYAGSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWSGFYKDSNYVAYASPT\r\nQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGVHDKPHESTTDNIVSFNDHYASTLWNVL\r\nPFSIVNVPTWLSHLPTGYGDGLTRVLDSKFYDLTSRDSTIIVANLSDPARANTWVQDLNR\r\nNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIG\r\nYQSTDKLVFKDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS",
"organism": "Pseudomonas sp. AMS8",
"uniprot_code": "E1B2U7",
"uniprot_range_first": 1,
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"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MGVFDYKNLGTEGSKALFADAMAITLYSYHNLDNGFAVGYQHNGFGLGLPATLVGALLGS\nTDSQGVIPGIPWNPDSEKAALEAVNKAGWTPISASTLGYGGKVDARGTFFGEKAGYTTAQ\nVEVLGKYDGDGKLLEIGIGFRGTSGPRETLITDSIGDLVSDLLAALGPKDYAKNYAGEAF\nGTLLKDVAAYAGSHGLTGKDVVVSGHSLGGLAVNSMADLSGNKWSGFYKDSNYVAYASPT\nQSSGDKVLNIGYENDPVFRALDGSSFNFSSLGVHDKPHESTTDNIVSFNDHYASTLWNVL\nPFSIVNVPTWLSHLPTGYGDGLTRVLDSKFYDLTSRDSTIIVANLSDPARANTWVQDLNR\nNAEPHKGNTFIIGSDGNDLIQGGKGVDFIEGGKGNDTIRDNSGHNTFLFGGQFGQDRVIG\nYQSTDKLVFKDVEGSADWRDHAKVVGGDTVLSFGADSVTLVGVGLAGVGGDGISIS",
"mw": 50.019,
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"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
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}
],
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"comment": null,
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"ph": 8.0,
"deuteration": null
},
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"name": "Cold-active AMS8 lipase in the presence of calcium (without polyhistidine affinity tag)",
"ext_coefficient": null,
"contrast": null,
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"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Thailand",
"full_name": "Synchrotron Light Research Institute",
"webpage": "https://www.slri.or.th"
}
],
"contributor_name": "Norhayati",
"contributor_surname": "Yaacob",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2018-03-06",
"storage_temperature": 10.0,
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"wavelength": 0.138,
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},
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}
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"description": null,
"experiment_description": "Synchrotron SAXS data from solutions of cold-active AMS8 lipase (without polyhistidine affinity tag) in 50 mM Tris HCl, 5 mM CaCl2, pH 8 were collected on the BL1.3W SAXS/WAXS beam line at the Synchrotron Light Research Institute (SLRI; Nakhon Ratchasima, Thailand) using a MAR 345 Image Plate detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.138 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 25°C. One 600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDEX6_datloglog_img.png",
"symmetry": null,
"last_modified": "2022-08-30T18:12:26.610492+02:00",
"bragg_peak": []
}
查看完整 manifest.json 原文
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