SASDEY6

Arabidopsis thaliana anaphase promoting complex/cyclosome subunit 4 (APC4-strep)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2020-12-12T16:01:14.893165+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Arabidopsis thaliana anaphase promoting complex/cyclosome subunit 4 (APC4-strep)

浓度8.1 – 8.1 缓冲液 / pH10 mM Tris 150 mM NaCl 1 mM TCEP / 8.0
实验温度20.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光1.0 s × 69

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Anaphase Promoting Complex/Cyclosome Subunit 4
查看序列
>APC4-STREPMSEMASDEEENIIPFQLQFDKPIPFQIKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGKPVTSLCWRPDGKAIAVGLEDGTISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTDESGNFSVYEDRTSRFFPPAPRPPKMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTGDRDGNICFSIFGIFQIGKINIHELSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMCTGELKDCDIKPREEKINVQDLPGLHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRASLSVMNKQWADAMKTFHEKFHSLSTLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNSLGEVGVKRVLKSVCGTGKELQQVVLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEMLLNEATENTGLLLVQVQRFMMVLSSVVQQFSNFFNWLVRSIKYLMQEPNDQLLSYNSELLVVFLKFLYDQDPVKDLLELSEAGDDIEIDLKTIGRVKELLQFGGFSECDFLQRTLAKEFQHMESSFKMAFQMPFTTISRKISCMKLLPLCPLQLSTTQTPTTIPMSLSFYKNELSDDTPCQSGYTDYISFQVPDETFPEISNCIGIAKGYKQNSNNEKNGYTSLEAVLLSVPNGYTCVDLSLYKDKELVLLLNKTNTDSEGSGEACMMVVQTGDLAFISISGSSSLNQWELEDLKGSIVNLEMENEKVRKVPHSVIAPLAVSASRGVACVFAERRRALVYILEEDEDEEISDEKDYDIPTTENLYFQGSAWSHPQFEKGGGSGGGSGGGSWSHPQFEK
proteinArabidopsis thalianaO654181–777dimer分子数 292.09 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1734[3]0.03555165–3.510133 1/nm含误差列缺失 01sasbdb/entries/y6/sasdey6/source/SASDEY6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)16.2—nm
i0Guinier49.90.042arbitrary
i0P(r)50.1—arbitrary
mwExperimental192.0—kDa
mwPorod162.0—kDa
porod_volumePorod259.0—nm³
rgGuinier4.640.01nm
rgP(r)4.702—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称10 mM Tris 150 mM NaCl 1 mM TCEP缓冲液浓度—
pH8.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-03-06储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数69
波长0.099样品-探测器距离2.867
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.036 – 3.51样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/y6/sasdey6/source/SASDEY6.zipdownloaded2954165ef390ae689d03ac908ace85831bf408b0be3bd2b4fb14d84c1e48e3ee237dfc下载查看原文件源站
pddfsasbdb/entries/y6/sasdey6/source/SASDEY6.outdownloaded642529921f2899b455675aacb23dad74d849aebd333286c32b10d85719ce92175315a下载查看原文件源站
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summarysasbdb/entries/y6/sasdey6/source/summary.jsondownloaded9516354c8c6486aea99482a7e4df347079e4c5f6dcf5889c746a0b978a12d195d1e9下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(6 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:174 个字段值
字段路径原始值
codeSASDEY6
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStructural Characterisation of the Arabidopsis thaliana APC4
project.publicationnull
project.statusreleased
project.submitted_date2018-10-29
project.released_date2020-12-12
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intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEY6_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEY6_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEY6_guinier_img.png
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAnaphase Promoting Complex/Cyclosome Subunit 4
experiment.sample.molecule[0].short_nameApc4
experiment.sample.molecule[0].sequence>APC4-strep MSEMASDEEENIIPFQLQFDKPIPFQIKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGKPVTSLCWRPDGKAIAVGLEDGTISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTDESGNFSVYEDRTSRFFPPAPRPPKMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTGDRDGNICFSIFGIFQIGKINIHELSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMCTGELKDCDIKPREEKINVQDLPGLHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRASLSVMNKQWADAMKTFHEKFHSLSTLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNSLGEVGVKRVLKSVCGTGKELQQVVLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEMLLNEATENTGLLLVQVQRFMMVLSSVVQQFSNFFNWLVRSIKYLMQEPNDQLLSYNSELLVVFLKFLYDQDPVKDLLELSEAGDDIEIDLKTIGRVKELLQFGGFSECDFLQRTLAKEFQHMESSFKMAFQMPFTTISRKISCMKLLPLCPLQLSTTQTPTTIPMSLSFYKNELSDDTPCQSGYTDYISFQVPDETFPEISNCIGIAKGYKQNSNNEKNGYTSLEAVLLSVPNGYTCVDLSLYKDKELVLLLNKTNTDSEGSGEACMMVVQTGDLAFISISGSSSLNQWELEDLKGSIVNLEMENEKVRKVPHSVIAPLAVSASRGVACVFAERRRALVYILEEDEDEEISDEKDYDIPTTENLYFQGSAWSHPQFEKGGGSGGGSGGGSWSHPQFEK
experiment.sample.molecule[0].organismArabidopsis thaliana
experiment.sample.molecule[0].uniprot_codeO65418
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last777
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSEMASDEEENIIPFQLQFDKPIPFQIKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWT ISPGKPVTSLCWRPDGKAIAVGLEDGTISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQS NTDESGNFSVYEDRTSRFFPPAPRPPKMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNIL CTGDRDGNICFSIFGIFQIGKINIHELSLPVPHLDEHASCKLFNASIYKVALSKDLCRLV VMCTGELKDCDIKPREEKINVQDLPGLHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEV IRASLSVMNKQWADAMKTFHEKFHSLSTLIIDNGLESSPQEEFLSLLGGARISPALNQFL VNSLGEVGVKRVLKSVCGTGKELQQVVLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGL DEMLLNEATENTGLLLVQVQRFMMVLSSVVQQFSNFFNWLVRSIKYLMQEPNDQLLSYNS ELLVVFLKFLYDQDPVKDLLELSEAGDDIEIDLKTIGRVKELLQFGGFSECDFLQRTLAK EFQHMESSFKMAFQMPFTTISRKISCMKLLPLCPLQLSTTQTPTTIPMSLSFYKNELSDD TPCQSGYTDYISFQVPDETFPEISNCIGIAKGYKQNSNNEKNGYTSLEAVLLSVPNGYTC VDLSLYKDKELVLLLNKTNTDSEGSGEACMMVVQTGDLAFISISGSSSLNQWELEDLKGS IVNLEMENEKVRKVPHSVIAPLAVSASRGVACVFAERRRALVYILEEDEDEEISDEK
experiment.sample.molecule[0].mw92.09
experiment.sample.molecule[0].total_mw184.18
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10 mM Tris 150 mM NaCl 1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameArabidopsis thaliana anaphase promoting complex/cyclosome subunit 4 (APC4-strep)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameVUB
experiment.contributor[0].affiliation[0].addressPleinlaan 2 1050 Brussel
experiment.contributor[0].affiliation[0].full_nameVrije Universiteit Brussel
experiment.contributor[0].affiliation[0].webpagehttp://www.vub.ac.be/
experiment.contributor[0].contributor_nameSteven
experiment.contributor[0].contributor_surnameDe Gieter
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-03-06
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames69
experiment.wavelength0.099
experiment.sample_detector_distance2.867
experiment.concentration_min8.1
experiment.concentration_max8.1
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.036
experiment.s_max3.51
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].commentnull
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fits[0].softwareCRYSOL
fits[0].chi_square_value3.102
fits[0].p_value0.0
fits[0].fit_residual_plotSASDEY6_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
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fits[1].models[0].type_of_modeldummy
fits[1].models[0].software_versionnull
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fits[1].models[0].model_mw160.835
fits[1].models[0].bead_radius3.9
fits[1].models[0].lognull
fits[1].models[0].symmetryP2
fits[1].models[0].commentnull
fits[1].models[0].user384
fits[1].fit_unit1/A
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fits[1].softwareDAMMIF
fits[1].chi_square_value0.788
fits[1].p_value0.000665
fits[1].fit_residual_plotSASDEY6_fit2_fitresiduals_img.png
fits[1].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDEY6_fit2.fir
fits[1].fit_lognull
fits[1].software_versionnull
fits[1].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of anaphase promoting complex/cyclosome subunit 4 in 10 mM Tris ,150 mM NaCl, 1 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed using the following parameters: Column type: Shodex KW404-4F; Injection volume: 50 µl; Sample injection concentration: 8.1 mg/ml. 69 successive 1 second frames were collected through the elution peak (measured at 20°C). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
tags[]
intensity_unitarbitrary
experimental_mw192.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw162.0
porod_mw_errornull
pddf_i050.1
pddf_i0_errornull
guinier_i049.9
guinier_i0_error0.042
pddf_rg4.702
pddf_rg_errornull
guinier_rg4.64
guinier_rg_error0.01
pddf_dmax16.2
pddf_dmax_errornull
porod_volume259.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first19
guinier_point_last53
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEY6_datloglog_img.png
symmetrynull
last_modified2020-12-12T16:01:14.893165+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEY6
statussuccess
started_at2026-08-11T15:18:41.565620+00:00
finished_at2026-08-11T15:18:51.433337+00:00
source_last_modified2020-12-12T16:01:14.893165+01:00
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查看完整 summary.json 原文
{
  "code": "SASDEY6",
  "status": "Published",
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  "angular_unit": "1/nm",
  "project": {
    "title": "Structural Characterisation of the Arabidopsis thaliana APC4",
    "publication": null,
    "status": "released",
    "submitted_date": "2018-10-29",
    "released_date": "2020-12-12"
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      "country": "France",
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          "organism": "Arabidopsis thaliana",
          "uniprot_code": "O65418",
          "uniprot_range_first": 1,
          "uniprot_range_last": 777,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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      "purity_method": null,
      "name": "Arabidopsis thaliana anaphase promoting complex/cyclosome subunit 4 (APC4-strep)",
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    "contributor": [
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        "affiliation": [
          {
            "short_name": "VUB",
            "address": "Pleinlaan 2 1050 Brussel ",
            "full_name": "Vrije Universiteit Brussel",
            "webpage": "http://www.vub.ac.be/"
          }
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        "contributor_name": "Steven",
        "contributor_surname": "De Gieter",
        "orcid": ""
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    "concentration_unit": null,
    "date": "2017-03-06",
    "storage_temperature": 4.0,
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    "number_of_frames": 69,
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  "last_modified": "2020-12-12T16:01:14.893165+01:00",
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}
查看完整 manifest.json 原文
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