SASDEZ7

C-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2020-08-28T15:28:10.062236+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · C-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II)

浓度— – 7.0 缓冲液 / pH25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4 / 5.5
实验温度20.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光1.0 s × 1

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Major prion protein
查看序列
GQGGSHSQWNKPSKPKTNMKHVAGAAAAGAVVGGLGGYMLGSAMSRPLIHFGNDYEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITVKQHTVRTTTKGENFTETDIKIMERVVEQMCITQYQRESQAYYQRGAS
proteinOvis ariesP2390794–234monomer分子数 116.085 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11029[3]0.1029649–4.927891 1/nm含误差列缺失 08sasbdb/entries/z7/sasdez7/source/SASDEZ7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)8.75—nm
i0Guinier10.240.02arbitrary
i0P(r)10.48—arbitrary
mwExperimental16.1—kDa
mwGuinier I(0)15.4—kDa
mwPorod15.375—kDa
porod_volumePorod30.75—nm³
rgGuinier2.230.01nm
rgP(r)2.405—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Ammonium Acetate, 250 mM NaCl, 0.001 mM CuSO4缓冲液浓度—
pH5.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2018-05-04储存 / 测量温度20.0 / 20.0
曝光时间1.0帧数1
波长0.099样品-探测器距离2.864
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.103 – 4.928样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDEZ7
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleDeciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain
project.publication.titleDeciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain
project.publication.author_listSalzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G
project.publication.journalBiophysical Journal
project.publication.doi10.1016/j.bpj.2019.12.025
project.publication.pmidnull
project.publication.published_date2020 Jan
project.statusreleased
project.submitted_date2018-11-13
project.released_date2019-11-25
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experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
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experiment.sample.nameC-terminal truncated ovine prion protein (94-234) with ARR polymorphism + Cu(II)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.contributor[0].affiliation[0].short_nameESRF
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experiment.contributor[0].affiliation[0].full_nameEuropean Synchrotron Radiation Facility
experiment.contributor[0].affiliation[0].webpagehttp://www.esrf.eu/
experiment.contributor[0].contributor_nameGabriele
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experiment.concentration_unitnull
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experiment.seccolumnnull
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pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of the C-terminal truncated ovine prion protein (94-234) with ARR polymorphism in 25 mM ammonium acetate, 250 mM NaCl, 0.001 mM CuSO4, pH 5.5 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column type, Superdex 200 5/150 GL (GE Healthcare); Flow rate: 0.15 mL/min; Total acquisition time: 25 min; Injection concentration: 7 mg/mL; Injection volume: 50 μL. The data obtained through the sample elution peak (873-925 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
tags[]
intensity_unitarbitrary
experimental_mw16.1
experimental_mw_errornull
guinier_i0_mw15.4
guinier_i0_mw_errornull
porod_mw15.375
porod_mw_errornull
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pddf_rg_errornull
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pddf_dmax_errornull
porod_volume30.75
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first38
guinier_point_last102
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDEZ7_datloglog_img.png
symmetrynull
last_modified2020-08-28T15:28:10.062236+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDEZ7
statussuccess
started_at2026-08-11T15:43:11.097130+00:00
finished_at2026-08-11T15:43:19.797822+00:00
source_last_modified2020-08-28T15:28:10.062236+02:00
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查看完整 summary.json 原文
{
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  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
    "publication": {
      "title": "Deciphering Copper Coordination in the Mammalian Prion Protein Amyloidogenic Domain",
      "author_list": "Salzano G, Brennich M, Mancini G, Tran T, Legname G, D’Angelo P, Giachin G",
      "journal": "Biophysical Journal",
      "doi": "10.1016/j.bpj.2019.12.025",
      "pmid": null,
      "published_date": "2020 Jan"
    },
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    "submitted_date": "2018-11-13",
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}
查看完整 manifest.json 原文
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