SASDF42

Human serum albumin in complex with somapacitan

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2020-11-27T12:03:56.572790+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Human serum albumin in complex with somapacitan

Concentration— – 6.3 Buffer / pH100 mM MES, 140 mM NaCl / 6.5
Experimental temperature25.0 Facility / beamlineNovo Nordisk A/S / Rigaku BioSAXS-2000
Wavelength0.1542 nmExposure3600.0 s × 6

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Human serum albumin
View sequence
DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL
proteinHomo sapiensP0276825–609monomerMolecule count 166.472 kDa
Somapacitan
View sequence
FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSCVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
proteinHomo sapiensP0124127–217dimerMolecule count 222.119 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1446[3]0.00372048–0.644951 1/AHas uncertainty columnMissing 014sasbdb/entries/42/sasdf42/source/SASDF42.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.92—Å
i0Guinier6.8430.02arbitrary
i0P(r)6.834—arbitrary
mwExperimental113.0—kDa
mwPorod126.0—kDa
porod_volumePorod202.0—ų
rgGuinier4.060.02Å
rgP(r)4.12—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name100 mM MES, 140 mM NaClBuffer concentration—
pH6.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-09-04Storage / measurement temperature— / 25.0
Exposure time3600.0Frames6
Wavelength0.1542Sample-to-detector distance—
SourceX-ray in houseDetectorPilatus 100K
Facility / beamlineNovo Nordisk A/S / Rigaku BioSAXS-2000 · Maaloev, Denmark
q range0.037 – 6.45Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:173 field values
Field pathRaw value
codeSASDF42
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleIdentification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.
project.publication.titleIdentification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.
project.publication.author_listJohansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
project.publication.journalBiochemistry
project.publication.doi10.1021/acs.biochem.0c00019
project.publication.pmid32208682
project.publication.published_date2020 Mar 25
project.statusreleased
project.submitted_date2019-01-28
project.released_date2020-03-31
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 100K
experiment.instrument.detector.resolution172.0
experiment.instrument.nameNovo Nordisk A/S
experiment.instrument.cityMaaloev
experiment.instrument.countryDenmark
experiment.instrument.beamline_nameRigaku BioSAXS-2000
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameHuman serum albumin
experiment.sample.molecule[0].short_nameHSA
experiment.sample.molecule[0].sequenceDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAE NCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEV DVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLP KLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTK VHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA DLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKC CAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVST PTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTES LVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKAT KEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP02768
experiment.sample.molecule[0].uniprot_range_first25
experiment.sample.molecule[0].uniprot_range_last609
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPF EDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEP ERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLF FAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAV ARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLK ECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYAR RHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFE QLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVV LNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTL SEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLV AASQAALGL
experiment.sample.molecule[0].mw66.472
experiment.sample.molecule[0].total_mw66.472
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameSomapacitan
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequenceFPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSCVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeP01241
experiment.sample.molecule[1].uniprot_range_first27
experiment.sample.molecule[1].uniprot_range_last217
experiment.sample.molecule[1].oligomerizationdimer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMATGSRTSLLLAFGLLCLPWLQEGSAFPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEA YIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLR SVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDD ALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
experiment.sample.molecule[1].mw22.119
experiment.sample.molecule[1].total_mw44.238
experiment.sample.molecule[1].number_molecules2
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name100 mM MES, 140 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameHuman serum albumin in complex with somapacitan
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressDenmark
experiment.contributor[0].affiliation[0].full_nameNovo Nordisk A/S
experiment.contributor[0].affiliation[0].webpagehttps://www.novonordisk.com/
experiment.contributor[0].contributor_nameEva
experiment.contributor[0].contributor_surnameJohansson
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-09-04
experiment.storage_temperaturenull
experiment.cell_temperature25.0
experiment.exposure_time3600.0
experiment.number_of_frames6
experiment.wavelength0.1542
experiment.sample_detector_distancenull
experiment.concentration_minnull
experiment.concentration_max6.3
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.037
experiment.s_max6.45
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
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fits[0].models[0].model_mw108.3
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fits[0].models[0].commentnull
fits[0].models[0].user408
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fits[0].softwareSASREF
fits[0].chi_square_value2.1025
fits[0].p_value0.002492
fits[0].fit_residual_plotSASDF42_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.5a
i0_calibration_standardnull
descriptionnull
experiment_descriptionSAXS data from solutions of HSA in complex with somapacitan in 100 mM MES, 140 mM NaCl, pH 6.5 were collected on a Rigaku BioSAXS-2000 instrument at Novo Nordisk A/S (Maaloev, Denmark) using a Pilatus 100K detector at a wavelength of λ = 0.1542 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.30 mg/ml was measured at 25°C. Six successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw113.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw126.0
porod_mw_errornull
pddf_i06.834
pddf_i0_errornull
guinier_i06.843
guinier_i0_error0.02
pddf_rg4.12
pddf_rg_errornull
guinier_rg4.06
guinier_rg_error0.02
pddf_dmax13.92
pddf_dmax_errornull
porod_volume202.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first6
guinier_point_last20
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDF42_datloglog_img.png
symmetrynull
last_modified2020-11-27T12:03:56.572790+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDF42
statussuccess
started_at2026-08-11T13:29:59.790861+00:00
finished_at2026-08-11T13:30:10.037269+00:00
source_last_modified2020-11-27T12:03:56.572790+01:00
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View the complete summary.json source
{
  "code": "SASDF42",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.",
    "publication": {
      "title": "Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.",
      "author_list": "Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P",
      "journal": "Biochemistry",
      "doi": "10.1021/acs.biochem.0c00019",
      "pmid": "32208682",
      "published_date": "2020 Mar 25"
    },
    "status": "released",
    "submitted_date": "2019-01-28",
    "released_date": "2020-03-31"
  },
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        "type": null,
        "name": "Pilatus 100K",
        "resolution": 172.0
      },
      "name": "Novo Nordisk A/S",
      "city": "Maaloev",
      "country": "Denmark",
      "beamline_name": "Rigaku BioSAXS-2000",
      "beam_geometry": null,
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        "affiliation": [
          {
            "short_name": null,
            "address": "Denmark",
            "full_name": "Novo Nordisk A/S",
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View the complete manifest.json source
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