SASDF48

Myotilin immunoglobulin domains Ig1Ig2 (250-498)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2020-10-28T14:25:06.632032+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Myotilin immunoglobulin domains Ig1Ig2 (250-498)

浓度— – 15.2 缓冲液 / pH20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT / 7.4
实验温度20.0 设备 / 束线ESRF / BM29
波长0.099 nm曝光15.0 s × 1

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Myotilin Ig1Ig2 (250-498)
查看序列
GPAMPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL
proteinHomo sapiensQ9UBF9250–498monomer分子数 128.31 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11036[3]0.03232795–4.493585 1/nm含误差列缺失 00sasbdb/entries/48/sasdf48/source/SASDF48.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)14.0—nm
i0Guinier14.870.01arbitrary
i0P(r)15.06—arbitrary
mwExperimental14.9—kDa
mwGuinier I(0)14.87—kDa
mwPorod23.0—kDa
porod_volumePorod37.0—nm³
rgGuinier3.220.02nm
rgP(r)3.418—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2019-06-27储存 / 测量温度— / 20.0
曝光时间15.0帧数1
波长0.099样品-探测器距离2.867
光源X-ray synchrotron探测器Pilatus 1M
机构 / 束线ESRF / BM29 · Grenoble, France
q 范围0.032 – 4.494样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDF48
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleMolecular basis of F-actin regulation and sarcomere assembly via myotilin
project.publication.titleMolecular basis of F-actin regulation and sarcomere assembly via myotilin
project.publication.author_listKostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
project.publication.journalPLOS Biology
project.publication.doi10.1371/journal.pbio.3001148
project.publication.pmidnull
project.publication.published_date2021 Apr 12
project.statusreleased
project.submitted_date2019-06-28
project.released_date2020-10-28
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDF48.dat
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experiment.instrument.detector.typeDectris
experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameESRF
experiment.instrument.cityGrenoble
experiment.instrument.countryFrance
experiment.instrument.beamline_nameBM29
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameMyotilin Ig1Ig2 (250-498)
experiment.sample.molecule[0].short_nameIg1Ig2 (250-498)
experiment.sample.molecule[0].sequenceGPAMPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ9UBF9
experiment.sample.molecule[0].uniprot_range_first250
experiment.sample.molecule[0].uniprot_range_last498
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFNYERPKHFIQSQNPCGSRLQPPGPETSSFSSQTKQSSIIIQPRQCTEQRFSASSTLSS HITMSSSAFPASPKQHAGSNPGQRVTTTYNQSPASFLSSILPSQPDYNSSKIPSAMDSNY QQSSAGQPINAKPSQTANAKPIPRTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLT YEEKMARRLLGPQNAAAVFQAQDDSGAQDSQQHNSEHARLQVPTSQVRSRSTSRGDVNDQ DAIQEKFYPPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIV SEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKV LEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGW YTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPE GEFQRLAAQSGLYESEEL
experiment.sample.molecule[0].mw28.31
experiment.sample.molecule[0].total_mw28.31
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMyotilin immunoglobulin domains Ig1Ig2 (250-498)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMelissa
experiment.contributor[0].contributor_surnameGraewert
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-06-27
experiment.storage_temperaturenull
experiment.cell_temperature20.0
experiment.exposure_time15.0
experiment.number_of_frames1
experiment.wavelength0.099
experiment.sample_detector_distance2.867
experiment.concentration_minnull
experiment.concentration_max15.2
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.032
experiment.s_max4.494
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Myotilin Ig1Ig2 (250-498) in 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.20 mg/ml was measured at 20°C. One 15 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw14.9
experimental_mw_errornull
guinier_i0_mw14.87
guinier_i0_mw_errornull
porod_mw23.0
porod_mw_errornull
pddf_i015.06
pddf_i0_errornull
guinier_i014.87
guinier_i0_error0.01
pddf_rg3.418
pddf_rg_errornull
guinier_rg3.22
guinier_rg_error0.02
pddf_dmax14.0
pddf_dmax_errornull
porod_volume37.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first29
guinier_point_last73
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDF48_datloglog_img.png
symmetrynull
last_modified2020-10-28T14:25:06.632032+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDF48
statussuccess
started_at2026-08-11T15:45:32.580073+00:00
finished_at2026-08-11T15:45:40.941030+00:00
source_last_modified2020-10-28T14:25:06.632032+01:00
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查看完整 summary.json 原文
{
  "code": "SASDF48",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Molecular basis of F-actin regulation and sarcomere assembly via myotilin",
    "publication": {
      "title": "Molecular basis of F-actin regulation and sarcomere assembly via myotilin",
      "author_list": "Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L",
      "journal": "PLOS Biology",
      "doi": "10.1371/journal.pbio.3001148",
      "pmid": null,
      "published_date": "2021 Apr 12"
    },
    "status": "released",
    "submitted_date": "2019-06-28",
    "released_date": "2020-10-28"
  },
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          "short_name": "Ig1Ig2 (250-498)",
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          "molecule_source": "biological",
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        "concentration_unit": null,
        "comment": null,
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        "pka": null,
        "ph": 7.4,
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      },
      "purity_method": null,
      "name": "Myotilin immunoglobulin domains Ig1Ig2 (250-498)",
      "ext_coefficient": null,
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            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
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        "contributor_name": "Melissa ",
        "contributor_surname": "Graewert",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2019-06-27",
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  "fits": [],
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  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of Myotilin Ig1Ig2 (250-498) in 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.20 mg/ml was measured at 20°C. One 15 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "pddf_point_first": null,
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  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDF48_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2020-10-28T14:25:06.632032+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
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