The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
Every source field is expanded here, including empty values, list members and fields already shown above.
View the complete summary.json source
{
"code": "SASDF63",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/nm",
"project": {
"title": "Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms",
"publication": {
"title": "Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms",
"author_list": "Duarte D, Lamontanara A, La Sala G, Jeong S, Sohn Y, Panjkovich A, Georgeon S, Kükenshöner T, Marcaida M, Pojer F, De Vivo M, Svergun D, Kim H, Dal Peraro M, Hantschel O",
"journal": "Nature Communications",
"doi": "10.1038/s41467-020-16128-5",
"pmid": null,
"published_date": "2020 May 8"
},
"status": "released",
"submitted_date": "2019-02-20",
"released_date": "2020-01-08"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDF63.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDF63.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF63_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF63_kratky_img.png",
"pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF63_pofr_img.png",
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF63_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDF63.sascif",
"experiment": {
"instrument": {
"detector": {
"type": "Dectris",
"name": "Pilatus 1M",
"resolution": 172.0
},
"name": "ESRF",
"city": "Grenoble",
"country": "France",
"beamline_name": "BM29",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Bruton's tyrosine kinase - Src homolgy domain 2 - kinase domain",
"short_name": "hBtk - SH2-KD",
"sequence": "GAMGSMTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES",
"organism": "Homo sapiens",
"uniprot_code": null,
"uniprot_range_first": 270,
"uniprot_range_last": 659,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": null,
"mw": 45.519,
"total_mw": 45.519,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "20mM Tris, 150mM NaCl, 1mM TCEP, 5% glycerol",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 7.5,
"deuteration": null
},
"purity_method": null,
"name": "Bruton's Tyrosine Kinase - SH2-kinase domain",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Switzerland",
"full_name": "École polytechnique fédérale de Lausanne (EPFL)",
"webpage": "https://www.epfl.ch"
}
],
"contributor_name": "Daniel",
"contributor_surname": "Duarte",
"orcid": "https://orcid.org/0000-0001-6730-7582"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2018-11-03",
"storage_temperature": 10.0,
"cell_temperature": 10.0,
"exposure_time": 2100.0,
"number_of_frames": 2100,
"wavelength": 0.09919,
"sample_detector_distance": 2.869,
"concentration_min": null,
"concentration_max": 20.4,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.059,
"s_max": 4.946,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDF63_fit1_model1_img.png",
"software": "DAMMIN",
"pdb_link": [],
"model_title": null,
"type_of_model": "dummy",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDF63_fit1_model1.pdb",
"model_mw": 39.093,
"bead_radius": 2.5,
"log": "https://www.sasbdb.org/media/log_files/Btk-2KD-SEC-DAMMIN.log",
"symmetry": null,
"comment": null,
"user": 406
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF63_fit1_fixed_fit_img.png",
"software": "DAMMIN",
"chi_square_value": 1.119,
"p_value": 0.237315,
"fit_residual_plot": "SASDF63_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDF63_fit1.fir",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Btk-2KD-SEC-DAMMIN.log",
"software_version": null,
"description": "Ab initio envelope reconstruction of human Btk SH2-KD."
},
{
"models": [],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF63_fit2_fixed_fit_img.png",
"software": "EOM/GAJOE",
"chi_square_value": 1.414,
"p_value": 0.007601,
"fit_residual_plot": "SASDF63_fit2_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDF63_fit2.fit",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/Btk-2-KD_SEC_EOM.log",
"software_version": null,
"description": "EOM analysis of human Btk SH2-KD using SH2 and KD structures as input (PDB 2GE9 and 1K2P)."
}
],
"estimated_volume_method": null,
"pddf_software": "ATSAS GNOM",
"pddf_software_version": "5.0",
"i0_calibration_standard": null,
"description": null,
"experiment_description": "Synchrotron SAXS data for SH2-kinase domain (amino acids 270-659) wild-type of human Bruton’s tyrosine kinase (Btk) in 20 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM TCEP, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a sample-detector distance of 2.849 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size exclusion chromatography (SEC) SAXS was performed by injecting 100µl of protein sample at 20mg/mL in a Superdex S200 Increase 10/300 column (GE Healthcare) at flow rate of 0.7mL/minute at room temperature. 2100 frames were collect during the 35 minutes total run. SEC-SAXS data frames 1031 - 1077 corresponding to monomeric peak were scaled, averaged and subtracted from an appropriate the solvent-blank to produce the final curve. The final scattering curve was analysed using PRIMUS software (ATSAS). The bead model depicts the DAMMIN ab initio reconstruction. Ensemble optimisation method (EOM) was performed using SH2 and kinase domain structures (2GE9 and 1K2P, PDB) to assess construct’s flexibility.",
"tags": [],
"intensity_unit": null,
"experimental_mw": 46.65,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": 40.0,
"porod_mw_error": null,
"pddf_i0": 85.59,
"pddf_i0_error": null,
"guinier_i0": 85.1,
"guinier_i0_error": 0.13,
"pddf_rg": 2.872,
"pddf_rg_error": null,
"guinier_rg": 2.8,
"guinier_rg_error": 0.01,
"pddf_dmax": 9.4,
"pddf_dmax_error": null,
"porod_volume": 64.47,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 33,
"guinier_point_last": 86,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDF63_datloglog_img.png",
"symmetry": null,
"last_modified": "2020-08-28T15:27:49.639423+02:00",
"bragg_peak": []
}View the complete manifest.json source
{
"code": "SASDF63",
"status": "success",
"started_at": "2026-08-11T13:53:19.931510+00:00",
"finished_at": "2026-08-11T13:53:29.188664+00:00",
"source_last_modified": "2020-08-28T15:27:49.639423+02:00",
"files": [
{
"status": "downloaded",
"url": "https://www.sasbdb.org/rest-api/entry/summary/SASDF63/",
"path": "/data/saxsdb/sasbdb/entries/63/sasdf63/source/summary.json",
"size": 8815,
"sha256": "1ab2edce93ad1fb092a74b34180f1f2c6d49909cdd64d2da5afba39ff5549814"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/intensities_files/SASDF63.dat",
"path": "/data/saxsdb/sasbdb/entries/63/sasdf63/source/SASDF63.dat",
"size": 50585,
"sha256": "4f981199ba45fb1f4bbb4051c56d606c68261d2913591a4be31d5087ee42e55d",
"role": "curve"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/p_of_R_files/SASDF63.out",
"path": "/data/saxsdb/sasbdb/entries/63/sasdf63/source/SASDF63.out",
"size": 56436,
"sha256": "8eb6915e7124265f0d41076a03c8a004d009eb0437e924e4d9a0d40a09044c93",
"role": "pddf"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDF63.sascif",
"path": "/data/saxsdb/sasbdb/entries/63/sasdf63/source/SASDF63.sascif",
"size": 272749,
"sha256": "1e2d00c37879a76c020b50a64fd0afee870b41766c56adb5bafe8be3e63d9b7a",
"role": "sascif"
},
{
"status": "downloaded",
"url": "https://www.sasbdb.org/media/zip_directories/SASDF63.zip",
"path": "/data/saxsdb/sasbdb/entries/63/sasdf63/source/SASDF63.zip",
"size": 64571,
"sha256": "970d978d457bcc28064411b808bb41e82eadfdc9999b7404345b5b89022a43a6",
"role": "full_entry_zip",
"zip_member_count": 6,
"zip_validation": "central_directory_readable"
}
]
}
