SASDF67

Complex of CBP KIX domain with BMAL1 (G490-L625) including C-terminal transactivation domain (TAD) (Mus musculus)

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:56:39.492710+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Complex of CBP KIX domain with BMAL1 (G490-L625) including C-terminal transactivation domain (TAD) (Mus musculus)

浓度— – 4.29 缓冲液 / pH25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol / 7.2
实验温度10.2 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.045 s × 30

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP)
查看序列
>GPGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRL
proteinMus musculusP45481586–672monomer分子数 110.486 kDa
Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD)
查看序列
>GGSGAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSTGLLPGQAQETPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL
proteinMus musculusQ9WTL8490–625monomer分子数 114.043 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11591[3]0.0473876–4.41951 1/nm含误差列缺失 00sasbdb/entries/67/sasdf67/source/SASDF67.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)17.5—nm
i0Guinier7308.7923.07—
i0P(r)7594.0——
mwExperimental33.1—kDa
rgGuinier3.750.35nm
rgP(r)4.264—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol缓冲液浓度—
pH7.2添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-05-27储存 / 测量温度10.2 / 10.2
曝光时间0.045帧数30
波长0.124样品-探测器距离3.0
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.047 – 4.42样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/67/sasdf67/source/SASDF67.zipdownloaded6723158b3dcd76304cbf3814852849e949b199f98360a8cc9902a2ade4f5872291212下载查看原文件源站
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summarysasbdb/entries/67/sasdf67/source/summary.jsondownloaded10637c3985d98acb88174d59eceab3e805443cadd3b8a16321499ec9ad248f92d0fb0下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:173 个字段值
字段路径原始值
codeSASDF67
statusPublished
type_of_curveSingle concentration
angular_unit1/nm
project.titleStructural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.
project.publication.titleStructural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.
project.publication.author_listGarg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.RA119.009845
project.publication.pmid31515273
project.publication.published_date2019 Sep 12
project.statusreleased
project.submitted_date2019-06-05
project.released_date2019-09-12
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDF67.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDF67.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF67_dat_img.png
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intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF67_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDF67.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.namePETRA III
experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameKinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP)
experiment.sample.molecule[0].short_nameCBP KIX domain
experiment.sample.molecule[0].sequence> GPGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANS RDEYYHLLAEKIYKIQKELEEKRRSRL
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeP45481
experiment.sample.molecule[0].uniprot_range_first586
experiment.sample.molecule[0].uniprot_range_last672
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMAENLLDGPPNPKRAKLSSPGFSANDNTDFGSLFDLENDLPDELIPNGELSLLNSGNLVP DAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSTNMASLGAMGKS PLNQGDSSTPNLPKQAASTSGPTPPASQALNPQAQKQVGLVTSSPATSQTGPGICMNANF NQTHPGLLNSNSGHSLMNQAQQGQAQVMNGSLGAAGRGRGAGMPYPAPAMQGATSSVLAE TLTQVSPQMAGHAGLNTAQAGGMTKMGMTGTTSPFGQPFSQTGGQQMGATGVNPQLASKQ SMVNSLPAFPTDIKNTSVTTVPNMSQLQTSVGIVPTQAIATGPTADPEKRKLIQQQLVLL LHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKN CTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGAGQQNATSLSNPNPIDPSSM QRAYAALGLPYMNQPQTQLQPQVPGQQPAQPPAHQQMRTLNALGNNPMSIPAGGITTDQQ PPNLISESALPTSLGATNPLMNDGSNSGNIGSLSTIPTAAPPSSTGVRKGWHEHVTQDLR SHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKI QKELEEKRRSRLHKQGILGNQPALPASGAQPPVIPPAQSVRPPNGPLPLPVNRMQVSQGM NSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMASVPGMAISPSRMPQPPNMMGTHAN NIMAQAPTQNQFLPQNQFPSSSGAMSVNSVGMGQPAAQAGVSQGQVPGAALPNPLNMLAP QASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHPTAPGMTPPQPAAPTQPSTPVSSGQTP TPTPGSVPSAAQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHTQPPGT PLSQAAASIDNRVPTPSSVTSAETSSQQPGPDVPMLEMKTEVQTDDAEPEPTESKGEPRS EMMEEDLQGSSQVKEETDTTEQKSEPMEVEEKKPEVKVEAKEEEENSSNDTASQSTSPSQ PRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIK RKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYC CGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQ TTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPR KENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRF VDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQNTALIAPHQIQGRVYISYLDSIHF FRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRL QEWYKKMLDKAFAERIINDYKDIFKQANEDRLTSAKELPYFEGDFWPNVLEESIKELEQE EEERKKEESTAASETPEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQ KLYATMEKHKEVFFVIHLHAGPVISTQPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFS SLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHT HKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMK RVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRL QQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVNMSPA GFPNVARTQPPTIVSAGKPTNQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRANINN GMPPGRAGMGTPGSQMTPVGLNVPRPNQVSGPVMSSMPPGQWQQAPIPQQQPMPGMPRPV MSMQAQAAVAGPRMPNVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAA FIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPPQP AMGGLNPQGQALNIMNPGHNPNMTNMNPQYREMVRRQLLQHQQQQQQQQQQQQQQQNSAS LAGGMAGHSQFQQPQGPGGYAPAMQQQRMQQHLPIQGSSMGQMAAPMGQLGQMGQPGLGA DSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSN QVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSMDQGHLG NPEQSAMLPQLNTPNRSALSSELSLVGDTTGDTLEKFVEGL
experiment.sample.molecule[0].mw10.486
experiment.sample.molecule[0].total_mw10.486
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameBrain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD)
experiment.sample.molecule[1].short_nameBMAL1-(490-625)
experiment.sample.molecule[1].sequence> GGSGAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASSPGGKKILNGGTPDIPSTGLLPGQAQETPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPSNDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL
experiment.sample.molecule[1].organismMus musculus
experiment.sample.molecule[1].uniprot_codeQ9WTL8
experiment.sample.molecule[1].uniprot_range_first490
experiment.sample.molecule[1].uniprot_range_last625
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMADQRMDISSTISDFMSPGPTDLLSGSLGTSGVDCNRKRKGSATDYQLDDFAFEESMDTD KDDPHGRLEYAEHQGRIKNAREAHSQIEKRRRDKMNSFIDELASLVPTCNAMSRKLDKLT VLRMAVQHMKTLRGATNPYTEANYKPTFLSDDELKHLILRAADGFLFVVGCDRGKILFVS ESVFKILNYSQNDLIGQSLFDYLHPKDIAKVKEQLSSSDTAPRERLIDAKTGLPVKTDIT PGPSRLCSGARRSFFCRMKCNRPSVKVEDKDFASTCSKKKDRKSFCTIHSTGYLKSWPPT KMGLDEDNEPDNEGCNLSCLVAIGRLHSHMVPQPANGEIRVKSMEYVSRHAIDGKFVFVD QRATAILAYLPQELLGTSCYEYFHQDDIGHLAECHRQVLQTREKITTNCYKFKIKDGSFI TLRSRWFSFMNPWTKEVEYIVSTNTVVLANVLEGGDPTFPQLTAPPHSMDSMLPSGEGGP KRTHPTVPGIPGGTRAGAGKIGRMIAEEIMEIHRIRGSSPSSCGSSPLNITSTPPPDASS PGGKKILNGGTPDIPSTGLLPGQAQETPGYPYSDSSSILGENPHIGIDMIDNDQGSSSPS NDEAAMAVIMSLLEADAGLGGPVDFSDLPWPL
experiment.sample.molecule[1].mw14.043
experiment.sample.molecule[1].total_mw14.043
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.2
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameComplex of CBP KIX domain with BMAL1 (G490-L625) including C-terminal transactivation domain (TAD) (Mus musculus)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressGermany
experiment.contributor[0].affiliation[0].full_nameInstitute of Molecular Biology Mainz
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameArchit
experiment.contributor[0].contributor_surnameGarg
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-05-27
experiment.storage_temperature10.2
experiment.cell_temperature10.2
experiment.exposure_time0.045
experiment.number_of_frames30
experiment.wavelength0.124
experiment.sample_detector_distance3.0
experiment.concentration_minnull
experiment.concentration_max4.29
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.047
experiment.s_max4.42
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].bead_radius3.5
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fits[0].models[0].commentnull
fits[0].models[0].user453
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fits[0].chi_square_value1.215
fits[0].p_value0.067951
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pddf_softwareATSAS GNOM
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tags[]
intensity_unitnull
experimental_mw33.1
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guinier_i0_error23.07
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guinier_rg3.75
guinier_rg_error0.35
pddf_dmax17.5
pddf_dmax_errornull
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estimated_volumenull
estimated_volume_errornull
guinier_point_first37
guinier_point_last109
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDF67_datloglog_img.png
symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDF67
statussuccess
started_at2026-08-11T15:22:55.582144+00:00
finished_at2026-08-11T15:23:04.423813+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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查看完整 summary.json 原文
{
  "code": "SASDF67",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.",
    "publication": {
      "title": "Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.",
      "author_list": "Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.RA119.009845",
      "pmid": "31515273",
      "published_date": "2019 Sep 12"
    },
    "status": "released",
    "submitted_date": "2019-06-05",
    "released_date": "2019-09-12"
  },
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF67_kratky_img.png",
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  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF67_guinier_img.png",
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      "country": "Germany",
      "beamline_name": "EMBL P12",
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      "type_of_source": "X-ray synchrotron",
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查看完整 manifest.json 原文
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  "started_at": "2026-08-11T15:22:55.582144+00:00",
  "finished_at": "2026-08-11T15:23:04.423813+00:00",
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