SASDF79

LIM domain-binding protein 1 (Ldb1) self association domain L87K

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2021-09-27T16:29:14.303024+02:00

A three point concentration series was collected at the Australian Synchrotron and reduced to I(s) versus s. Parameters derived from the Guinier analysis showed a small concentration dependence of systematically increasing molecular mass (M) values from I(0). The M values were consistent with a predominantly dimeric for for all samples, the data suggest there may be somewhat less than complete dimerization at the lower concentration values, and potentially some further associations at the highest concentration values. Data is provided for the sample at 0.4 mg/ml where the M value is consistent with dimer.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · LIM domain-binding protein 1 (Ldb1) self association domain L87K

Concentration— – 0.4 Buffer / pH20 mM Tris, 150 mM NaCl, 1 mM TCEP, / 8.0
Experimental temperature13.6 Facility / beamlineAustralian Synchrotron / SAXS/WAXS
Wavelength0.10332 nmExposure1.0 s × 24

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
LIM domain-binding protein 1, L87K
View sequence
GELGSPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTITFCLEDGPKRYTIGRTKIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQVCVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLS
proteinMus musculusP7066214–200dimerMolecule count 222.595 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1825[3]0.0064398025–0.3276825 1/AHas uncertainty columnMissing 061sasbdb/entries/79/sasdf79/source/SASDF79.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)7.64—Å
i0Guinier0.01347—1/cm
i0P(r)0.01344—1/cm
mwExperimental49.17—kDa
mwGuinier I(0)50.834—kDa
mwPorod49.0—kDa
porod_volumePorod78.4—ų
rgGuinier2.430.8Å
rgP(r)2.42—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris, 150 mM NaCl, 1 mM TCEP,Buffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2015-11-19Storage / measurement temperature22.0 / 13.6
Exposure time1.0Frames24
Wavelength0.10332Sample-to-detector distance2.68
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineAustralian Synchrotron / SAXS/WAXS · Melbourne, Australia
q range0.064 – 3.277Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:174 field values
Field pathRaw value
codeSASDF79
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleSelf association of LIM domain binding protein 1, LDB1
project.publicationnull
project.statusreleased
project.submitted_date2019-07-16
project.released_date2021-09-27
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experiment.instrument.detector.namePilatus 1M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAustralian Synchrotron
experiment.instrument.cityMelbourne
experiment.instrument.countryAustralia
experiment.instrument.beamline_nameSAXS/WAXS
experiment.instrument.beam_geometryPoint
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourceTrue
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameLIM domain-binding protein 1, L87K
experiment.sample.molecule[0].short_nameLDB1-L87K
experiment.sample.molecule[0].sequenceGELGSPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTITFCLEDGPKRYTIGRTKIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQVCVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLS
experiment.sample.molecule[0].organismMus musculus
experiment.sample.molecule[0].uniprot_codeP70662
experiment.sample.molecule[0].uniprot_range_first14
experiment.sample.molecule[0].uniprot_range_last200
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSVGCACPGCSSKSFKLYSPKEPPNGNAFPPFHPGTMLDRDVGPTPMYPPTYLEPGIGRH TPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTITFCLEDGPKRYTIG RTLIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQVCVEG RLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLSNSTL NYLRLCVILEPMQELMSRHKTYSLSPRDCLKTCLFQKWQRMVAPPAEPARQQPSKRRKRK MSGGSTMSSGGGNTNNSNSKKKSPASTFALSSQVPDVMVVGEPTLMGGEFGDEDERLITR LENTQFDAANGIDDEDSFNNSPALGANSPWNSKPPSSQESKSENPTSQASQ
experiment.sample.molecule[0].mw22.595
experiment.sample.molecule[0].total_mw45.19
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris, 150 mM NaCl, 1 mM TCEP,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameLIM domain-binding protein 1 (Ldb1) self association domain L87K
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressSydney, Australia
experiment.contributor[0].affiliation[0].full_nameSchool of Life and Environmental Science, University of Sydney
experiment.contributor[0].affiliation[0].webpagehttp://sydney.edu.au/science/life-environment/
experiment.contributor[0].contributor_nameJacqueline
experiment.contributor[0].contributor_surnameMatthews
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8518-3472
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-11-19
experiment.storage_temperature22.0
experiment.cell_temperature13.6
experiment.exposure_time1.0
experiment.number_of_frames24
experiment.wavelength0.10332
experiment.sample_detector_distance2.68
experiment.concentration_minnull
experiment.concentration_max0.4
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.064
experiment.s_max3.277
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwarePDB (Protein Data Bank)
fits[0].models[0].pdb_link[]
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fits[0].models[0].type_of_modelatomic
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fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentThe asymmetric unit is monomeric with 2 dimers by symmetry. Missing ~30% density.
fits[0].models[0].user466
fits[0].fit_unit1/A
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fits[0].softwareCORAL
fits[0].chi_square_value1.15
fits[0].p_value0.802642
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fits[0].software_version5
fits[0].descriptionL87K HH Model
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fits[1].models[0].user466
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fits[1].softwareCORAL
fits[1].chi_square_value1.86
fits[1].p_value0.0
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fits[1].software_version5
fits[1].descriptionL87Q HT model
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionA three point concentration series was collected at the Australian Synchrotron and reduced to I(s) versus s. Parameters derived from the Guinier analysis showed a small concentration dependence of systematically increasing molecular mass (M) values from I(0). The M values were consistent with a predominantly dimeric for for all samples, the data suggest there may be somewhat less than complete dimerization at the lower concentration values, and potentially some further associations at the highest concentration values. Data is provided for the sample at 0.4 mg/ml where the M value is consistent with dimer.
experiment_descriptionSynchrotron SAXS data from solutions of Ldb1 self association domain (L87K) in 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 8 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.40 mg/ml was measured at 13.6°C. 24 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unit1/cm
experimental_mw49.17
experimental_mw_errornull
guinier_i0_mw50.834
guinier_i0_mw_errornull
porod_mw49.0
porod_mw_errornull
pddf_i00.01344
pddf_i0_errornull
guinier_i00.01347
guinier_i0_errornull
pddf_rg2.42
pddf_rg_errornull
guinier_rg2.43
guinier_rg_error0.8
pddf_dmax7.64
pddf_dmax_errornull
porod_volume78.4
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first7
guinier_point_last121
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDF79_datloglog_img.png
symmetrynull
last_modified2021-09-27T16:29:14.303024+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDF79
statussuccess
started_at2026-08-11T16:07:39.318321+00:00
finished_at2026-08-11T16:07:52.166545+00:00
source_last_modified2021-09-27T16:29:14.303024+02:00
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View the complete summary.json source
{
  "code": "SASDF79",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Self association of LIM domain binding protein 1, LDB1",
    "publication": null,
    "status": "released",
    "submitted_date": "2019-07-16",
    "released_date": "2021-09-27"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDF79.dat",
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  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF79_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF79_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDF79.sascif",
  "experiment": {
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      "country": "Australia",
      "beamline_name": "SAXS/WAXS",
      "beam_geometry": "Point",
      "type_of_source": "X-ray synchrotron",
      "point_source": true,
      "line_collimation": null,
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    },
    "sample": {
      "molecule": [
        {
          "long_name": "LIM domain-binding protein 1, L87K",
          "short_name": "LDB1-L87K",
          "sequence": "GELGSPTYLEPGIGRHTPYGNQTDYRIFELNKRLQNWTEECDNLWWDAFTTEFFEDDAMLTITFCLEDGPKRYTIGRTKIPRYFRSIFEGGATELYYVLKHPKEAFHSNFVSLDCDQGSMVTQHGKPMFTQVCVEGRLYLEFMFDDMMRIKTWHFSIRQHRELIPRSILAMHAQDPQMLDQLSKNITRCGLS",
          "organism": "Mus musculus",
          "uniprot_code": "P70662",
          "uniprot_range_first": 14,
          "uniprot_range_last": 200,
          "oligomerization": "dimer",
          "molecular_type": "protein",
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        }
      ],
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        "name": "20 mM Tris, 150 mM NaCl, 1 mM TCEP,",
        "concentration_unit": null,
        "comment": null,
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        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "LIM domain-binding protein 1 (Ldb1) self association domain L87K",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
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    },
    "contributor": [
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        "affiliation": [
          {
            "short_name": null,
            "address": "Sydney, Australia",
            "full_name": "School of Life and Environmental Science, University of Sydney",
            "webpage": "http://sydney.edu.au/science/life-environment/"
          }
        ],
        "contributor_name": "Jacqueline",
        "contributor_surname": "Matthews",
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      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
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    "storage_temperature": 22.0,
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          "user": 466
        }
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      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF79_fit1_fixed_fit_img.png",
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  "last_modified": "2021-09-27T16:29:14.303024+02:00",
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}
View the complete manifest.json source
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