SASDF82

Urokinase plasminogen activator surface receptor, uPAR H47C-N259C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).

Data type: SASBDB experimental data Status: Published Curve type: Extrapolated to infinite dilution Last updated: 2023-05-25T11:21:52.654103+02:00

Introduced disulfide in the first LU domain, DI, of uPAR (H47C-N259C).

▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Urokinase plasminogen activator surface receptor, uPAR H47C-N259C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).

Concentration2.3 – 7.5 Buffer / pH20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4, / 7.4
Experimental temperature10.0 Facility / beamlineDORIS III, DESY / EMBL X33
Wavelength0.15 nmExposure30.0 s × 4

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Urokinase plasminogen activator surface receptor
View sequence
>SP|Q03405|UPAR_HUMANUROKINASEPLASMINOGENACTIVATORSURFACERECEPTOROS=HOMOSAPIENSGN=PLAURPE=1SV=1MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT
proteinHomo sapiensQ03405—–—monomerMolecule count 136.98 kDa
Urokinase-type plasminogen activator (Amino Terminal Fragment)
View sequence
MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHNYCRNPDNRRRPWCYVQVGLKPLV
proteinHomo sapiensP007491–143monomerMolecule count 116.099 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12293[3]0.190918–6.34517 1/nmHas uncertainty columnMissing 01sasbdb/entries/82/sasdf82/source/SASDF82.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)8.2—nm
i0Guinier57.10.1arbitrary
i0P(r)57.15—arbitrary
mwExperimental58.6—kDa
mwGuinier I(0)58.6—kDa
mwPorod63.75—kDa
porod_volumePorod102.0—nm³
rgGuinier2.560.1nm
rgP(r)2.577—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

Fit 1 · DAMMIN 5.3

χ²: 1.555CorMap p: 0.000174Unit: 1/A

Refined DAMMIN model from average volume (10 DAMMIF iterations)

ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—dummyDAMMIN —50.81—Model Image Log
▶

4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4,Buffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2011-06-18Storage / measurement temperature10.0 / 10.0
Exposure time30.0Frames4
Wavelength0.15Sample-to-detector distance2.7
SourceX-ray synchrotronDetectorPilatus 1M-W
Facility / beamlineDORIS III, DESY / EMBL X33 · Hamburg, Germany
q range0.191 – 6.345Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
curvesasbdb/entries/82/sasdf82/source/SASDF82.datdownloaded9859949bcafa68d44a726f38c8dcd7c193c5e577f506f10c9b348a1fe02aa7cef41b5DownloadView source fileSource site
full_entry_zipsasbdb/entries/82/sasdf82/source/SASDF82.zipdownloaded67281e374d15d9b4218e25ac3fbeb1b18925d8118f6d2217386c6c837025fb62426f8DownloadView source fileSource site
pddfsasbdb/entries/82/sasdf82/source/SASDF82.outdownloaded709897663314a5360df719766a74e5f4e4c763f71742d1d5b6ad6ef6d6776ed47ac16DownloadView source fileSource site
sascifsasbdb/entries/82/sasdf82/source/SASDF82.sascifdownloaded333637115de66ba74b50e32b67b4289a2a6272229dd128ab5ee5f1d4364f53269675c3DownloadView source fileSource site
summarysasbdb/entries/82/sasdf82/source/summary.jsondownloaded9716c67063ee9071b80a15f55aaa1099644033502fba78b7d2dd088d8dacfc8418a3DownloadView source fileSource site
curve:source record
{
  "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.dat",
  "role": "curve",
  "sha256": "49bcafa68d44a726f38c8dcd7c193c5e577f506f10c9b348a1fe02aa7cef41b5",
  "size": 98599,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDF82.dat"
}
full_entry_zip:source record and ZIP contents (4 items)
{
  "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.zip",
  "role": "full_entry_zip",
  "sha256": "e374d15d9b4218e25ac3fbeb1b18925d8118f6d2217386c6c837025fb62426f8",
  "size": 67281,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDF82.zip",
  "zip_member_count": 4,
  "zip_members": [
    {
      "crc32": "5dcf6fb2",
      "name": "SASDF82/experimental_data/SASDF82.dat",
      "size": 98599
    },
    {
      "crc32": "cab3689e",
      "name": "SASDF82/pddf/SASDF82.out",
      "size": 70989
    },
    {
      "crc32": "71eff922",
      "name": "SASDF82/SASDF82_fit1.fir",
      "size": 45806
    },
    {
      "crc32": "cc124cf4",
      "name": "SASDF82/SASDF82_fit1_model1.pdb",
      "size": 87472
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:source record
{
  "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.out",
  "role": "pddf",
  "sha256": "7663314a5360df719766a74e5f4e4c763f71742d1d5b6ad6ef6d6776ed47ac16",
  "size": 70989,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDF82.out"
}
sascif:source record
{
  "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.sascif",
  "role": "sascif",
  "sha256": "115de66ba74b50e32b67b4289a2a6272229dd128ab5ee5f1d4364f53269675c3",
  "size": 333637,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDF82.sascif"
}
summary:source record
{
  "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/summary.json",
  "sha256": "c67063ee9071b80a15f55aaa1099644033502fba78b7d2dd088d8dacfc8418a3",
  "size": 9716,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDF82/"
}

All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:173 field values
Field pathRaw value
codeSASDF82
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/nm
project.titleDid evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?
project.publication.titleDid evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?
project.publication.author_listLeth JM, Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M
project.publication.journalJ Biol Chem
project.publication.doi10.1074/jbc.RA119.007847
project.publication.pmid30894413
project.publication.published_date2019 Mar 20
project.statusreleased
project.submitted_date2019-01-30
project.released_date2019-03-20
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDF82.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDF82.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF82_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF82_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF82_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF82_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDF82.sascif
experiment.instrument.detector.typeCustom-built
experiment.instrument.detector.namePilatus 1M-W
experiment.instrument.detector.resolution0.172
experiment.instrument.nameDORIS III, DESY
experiment.instrument.cityHamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL X33
experiment.instrument.beam_geometry0.60
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameUrokinase plasminogen activator surface receptor
experiment.sample.molecule[0].short_nameuPAR
experiment.sample.molecule[0].sequence>sp|Q03405|UPAR_HUMAN Urokinase plasminogen activator surface receptor OS=Homo sapiens GN=PLAUR PE=1 SV=1 MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL ELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC GSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG FHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP MNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV QYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ03405
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL ELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC GSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG FHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP MNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV QYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT
experiment.sample.molecule[0].mw36.98
experiment.sample.molecule[0].total_mw36.98
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_statenull
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameUrokinase-type plasminogen activator (Amino Terminal Fragment)
experiment.sample.molecule[1].short_nameATF
experiment.sample.molecule[1].sequenceMRALLARLLL CVLVVSDSKG SNELHQVPSN CDCLNGGTCV SNKYFSNIHW CNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL QQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLV
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeP00749
experiment.sample.molecule[1].uniprot_range_first1
experiment.sample.molecule[1].uniprot_range_last143
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQ HCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHN YCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKII GGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLG RSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICL PSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKML CAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIR SHTKEENGLAL
experiment.sample.molecule[1].mw16.099
experiment.sample.molecule[1].total_mw16.099
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameUrokinase plasminogen activator surface receptor, uPAR H47C-N259C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameHaydyn
experiment.contributor[0].contributor_surnameMertens
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2011-06-18
experiment.storage_temperature10.0
experiment.cell_temperature10.0
experiment.exposure_time30.0
experiment.number_of_frames4
experiment.wavelength0.15
experiment.sample_detector_distance2.7
experiment.concentration_min2.3
experiment.concentration_max7.5
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.191
experiment.s_max6.345
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDF82_fit1_model1_img.png
fits[0].models[0].softwareDAMMIN
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldummy
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDF82_fit1_model1.pdb
fits[0].models[0].model_mw50.81
fits[0].models[0].bead_radius2.5
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/ref_br4q5tj.log
fits[0].models[0].symmetrynull
fits[0].models[0].commentRefined average model
fits[0].models[0].user12
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/ref_rGToAU1_fit_img.png
fits[0].softwareDAMMIN
fits[0].chi_square_value1.555
fits[0].p_value0.000174
fits[0].fit_residual_plotref_rGToAU1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDF82_fit1.fir
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/ref_QhCVSy5.log
fits[0].software_version5.3
fits[0].descriptionRefined DAMMIN model from average volume (10 DAMMIF iterations)
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionIntroduced disulfide in the first LU domain, DI, of uPAR (H47C-N259C).
experiment_descriptionSynchrotron SAXS data from solutions of uPAR H47C-N259C in complex with uPA-ATF in 20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4, pH 7.4 were collected on the EMBL X33 beam line at DORIS III (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 7.5 mg/ml were measured at 10°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
tags[0]X33
intensity_unitarbitrary
experimental_mw58.6
experimental_mw_errornull
guinier_i0_mw58.6
guinier_i0_mw_errornull
porod_mw63.75
porod_mw_errornull
pddf_i057.15
pddf_i0_errornull
guinier_i057.1
guinier_i0_error0.1
pddf_rg2.577
pddf_rg_errornull
guinier_rg2.56
guinier_rg_error0.1
pddf_dmax8.2
pddf_dmax_errornull
porod_volume102.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last118
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDF82_datloglog_img.png
symmetrynull
last_modified2023-05-25T11:21:52.654103+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDF82
statussuccess
started_at2026-08-11T13:32:36.838538+00:00
finished_at2026-08-11T13:32:49.704504+00:00
source_last_modified2023-05-25T11:21:52.654103+02:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDF82/
files[0].path/data/saxsdb/sasbdb/entries/82/sasdf82/source/summary.json
files[0].size9716
files[0].sha256c67063ee9071b80a15f55aaa1099644033502fba78b7d2dd088d8dacfc8418a3
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDF82.dat
files[1].path/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.dat
files[1].size98599
files[1].sha25649bcafa68d44a726f38c8dcd7c193c5e577f506f10c9b348a1fe02aa7cef41b5
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDF82.out
files[2].path/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.out
files[2].size70989
files[2].sha2567663314a5360df719766a74e5f4e4c763f71742d1d5b6ad6ef6d6776ed47ac16
files[2].rolepddf
files[3].statusdownloaded
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDF82.sascif
files[3].path/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.sascif
files[3].size333637
files[3].sha256115de66ba74b50e32b67b4289a2a6272229dd128ab5ee5f1d4364f53269675c3
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDF82.zip
files[4].path/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.zip
files[4].size67281
files[4].sha256e374d15d9b4218e25ac3fbeb1b18925d8118f6d2217386c6c837025fb62426f8
files[4].rolefull_entry_zip
files[4].zip_member_count4
files[4].zip_validationcentral_directory_readable
View the complete summary.json source
{
  "code": "SASDF82",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/nm",
  "project": {
    "title": "Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?",
    "publication": {
      "title": "Did evolution create a flexible ligand-binding cavity in the urokinase receptor through deletion of a plesiotypic disulfide bond?",
      "author_list": "Leth JM, Mertens HDT, Leth-Espensen KZ, Jørgensen TJD, Ploug M",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.RA119.007847",
      "pmid": "30894413",
      "published_date": "2019 Mar 20"
    },
    "status": "released",
    "submitted_date": "2019-01-30",
    "released_date": "2019-03-20"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDF82.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDF82.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF82_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF82_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF82_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF82_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDF82.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "Custom-built",
        "name": "Pilatus 1M-W",
        "resolution": 0.172
      },
      "name": "DORIS III, DESY",
      "city": "Hamburg",
      "country": "Germany",
      "beamline_name": "EMBL X33",
      "beam_geometry": "0.60",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Urokinase plasminogen activator surface receptor",
          "short_name": "uPAR",
          "sequence": ">sp|Q03405|UPAR_HUMAN Urokinase plasminogen activator surface receptor OS=Homo sapiens GN=PLAUR PE=1 SV=1\nMGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL\nELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC\nGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG\nFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP\nMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV\nQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT\n\n",
          "organism": "Homo sapiens",
          "uniprot_code": "Q03405",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEEL\nELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISC\nGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNG\nFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGP\nMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDV\nQYRSGAAPQPGPAHLSLTITLLMTARLWGGTLLWT",
          "mw": 36.98,
          "total_mw": 36.98,
          "number_molecules": 1,
          "complex_state": null,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Urokinase-type plasminogen activator (Amino Terminal Fragment)",
          "short_name": "ATF",
          "sequence": "MRALLARLLL CVLVVSDSKG SNELHQVPSN CDCLNGGTCV SNKYFSNIHW \r\nCNCPKKFGGQ HCEIDKSKTC YEGNGHFYRG KASTDTMGRP CLPWNSATVL \r\nQQTYHAHRSD ALQLGLGKHN YCRNPDNRRR PWCYVQVGLK PLV",
          "organism": "Homo sapiens",
          "uniprot_code": "P00749",
          "uniprot_range_first": 1,
          "uniprot_range_last": 143,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MRALLARLLLCVLVVSDSKGSNELHQVPSNCDCLNGGTCVSNKYFSNIHWCNCPKKFGGQ\nHCEIDKSKTCYEGNGHFYRGKASTDTMGRPCLPWNSATVLQQTYHAHRSDALQLGLGKHN\nYCRNPDNRRRPWCYVQVGLKPLVQECMVHDCADGKKPSSPPEELKFQCGQKTLRPRFKII\nGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLISPCWVISATHCFIDYPKKEDYIVYLG\nRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPSRTIQTICL\nPSMYNDPQFGTSCEITGFGKENSTDYLYPEQLKMTVVKLISHRECQQPHYYGSEVTTKML\nCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYTRVSHFLPWIR\nSHTKEENGLAL",
          "mw": 16.099,
          "total_mw": 16.099,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4,",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Urokinase plasminogen activator surface receptor, uPAR H47C-N259C, complex with urokinase-type plasminogen activator (Amino Terminal Fragment, ATF).",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "EMBL-Hamburg",
            "address": "Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany",
            "full_name": "European Molecular Biology Laboratory (EMBL) - Hamburg outstation",
            "webpage": "http://www.embl-hamburg.de/index.php"
          }
        ],
        "contributor_name": "Haydyn",
        "contributor_surname": "Mertens",
        "orcid": null
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2011-06-18",
    "storage_temperature": 10.0,
    "cell_temperature": 10.0,
    "exposure_time": 30.0,
    "number_of_frames": 4,
    "wavelength": 0.15,
    "sample_detector_distance": 2.7,
    "concentration_min": 2.3,
    "concentration_max": 7.5,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.191,
    "s_max": 6.345,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDF82_fit1_model1_img.png",
          "software": "DAMMIN",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDF82_fit1_model1.pdb",
          "model_mw": 50.81,
          "bead_radius": 2.5,
          "log": "https://www.sasbdb.org/media/log_files/ref_br4q5tj.log",
          "symmetry": null,
          "comment": "Refined average model",
          "user": 12
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/ref_rGToAU1_fit_img.png",
      "software": "DAMMIN",
      "chi_square_value": 1.555,
      "p_value": 0.000174,
      "fit_residual_plot": "ref_rGToAU1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDF82_fit1.fir",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/ref_QhCVSy5.log",
      "software_version": "5.3",
      "description": "Refined DAMMIN model from average volume (10 DAMMIF iterations)"
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "Introduced disulfide in the first LU domain, DI, of uPAR (H47C-N259C).",
  "experiment_description": "Synchrotron SAXS data from solutions of uPAR H47C-N259C in complex with uPA-ATF in 20 mM PBS, 5 %(v/v) glycerol, 50 mM NaSO4, pH 7.4 were collected on the EMBL X33 beam line at DORIS III (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.3 and 7.5 mg/ml were measured at 10°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.",
  "tags": [
    "X33"
  ],
  "intensity_unit": "arbitrary",
  "experimental_mw": 58.6,
  "experimental_mw_error": null,
  "guinier_i0_mw": 58.6,
  "guinier_i0_mw_error": null,
  "porod_mw": 63.75,
  "porod_mw_error": null,
  "pddf_i0": 57.15,
  "pddf_i0_error": null,
  "guinier_i0": 57.1,
  "guinier_i0_error": 0.1,
  "pddf_rg": 2.577,
  "pddf_rg_error": null,
  "guinier_rg": 2.56,
  "guinier_rg_error": 0.1,
  "pddf_dmax": 8.2,
  "pddf_dmax_error": null,
  "porod_volume": 102.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 118,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDF82_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2023-05-25T11:21:52.654103+02:00",
  "bragg_peak": []
}
View the complete manifest.json source
{
  "code": "SASDF82",
  "status": "success",
  "started_at": "2026-08-11T13:32:36.838538+00:00",
  "finished_at": "2026-08-11T13:32:49.704504+00:00",
  "source_last_modified": "2023-05-25T11:21:52.654103+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDF82/",
      "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/summary.json",
      "size": 9716,
      "sha256": "c67063ee9071b80a15f55aaa1099644033502fba78b7d2dd088d8dacfc8418a3"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDF82.dat",
      "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.dat",
      "size": 98599,
      "sha256": "49bcafa68d44a726f38c8dcd7c193c5e577f506f10c9b348a1fe02aa7cef41b5",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDF82.out",
      "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.out",
      "size": 70989,
      "sha256": "7663314a5360df719766a74e5f4e4c763f71742d1d5b6ad6ef6d6776ed47ac16",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDF82.sascif",
      "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.sascif",
      "size": 333637,
      "sha256": "115de66ba74b50e32b67b4289a2a6272229dd128ab5ee5f1d4364f53269675c3",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDF82.zip",
      "path": "/data/saxsdb/sasbdb/entries/82/sasdf82/source/SASDF82.zip",
      "size": 67281,
      "sha256": "e374d15d9b4218e25ac3fbeb1b18925d8118f6d2217386c6c837025fb62426f8",
      "role": "full_entry_zip",
      "zip_member_count": 4,
      "zip_validation": "central_directory_readable"
    }
  ]
}