SASDF87

Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2020-02-03T17:28:44.674498+01:00

Molecular weight estimated from experimental data using I(0) value from GNOM analysis. Submitted SANS data has been normalized to protein concentration of 1 mg/ml. Protein (3.33 mg/ml) was used during SANS experiments

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Mosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O

Concentration— – 3.3 Buffer / pH25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O / 7.5
Experimental temperature25.0 Facility / beamlineDhruva Reactor, Bhabha Atomic Research Centre / SANS-I facility
Wavelength0.52 nmExposure43200.0 s × 1

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Binary toxin receptor Cqm1 protein
View sequence
>VTO94530.1CQM1RECEPTOR,MATUREFORM(23-560),PARTIAL[SYNTHETICGENE](WITHTAG)MHHHHHHSSGVDLGTENLYFQSMREPDSKDWYQHATFYQIYPRSFLDSNGDGIGDLAGITSKMKYLADIGIDATWLSPPFKSPLKDFGYDVSDFYAIQPEYGNLTDFDKLVEESHKNGIKLMLDFIPNHSSDQHEWFVKSVVRDPEYSDFYVWRPPATGGGPPNNWISVFGGSAWTYNQARGEYYLHQFTPQQPDLNYRNPKVLAEMTKMLFFWLDRGVDGFRLDAINHMFEDEQFRDEPLSGWGQPGEYDSLDHIYTKDIPDVYDVVYNWRDQMDKYSAEKGRTIILMTEAYSSIEGTMLYYESADRKRQGAHMPFNFQLIYDFKKEQNAVGLKSSIDWWMNNMPARHTPSWVAGSHDHSRVASRVGLDRVDQVMTLLHTLPGTSITYYGEEVAMQDFKEAQQFDNRDPNRTPMQWDSSTSAGFSTNTNTWLRVHPDYARYNVDVMQKNPQSTFHHFQHLTKLRRHRTMQSGEYVHKTVGTKVYALLRELRGEDSFLTVLNMAGAEDTVDLGDFVNLPQKMRVEVAQPNSKSKAGNEVDISKLTLGPYDSVVLRATVSSA
proteinsynthetic construct——–—dimerMolecule count 264.67 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1100[3]0.01619–0.19905 1/AHas uncertainty columnMissing 00sasbdb/entries/87/sasdf87/source/SASDF87.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)9.66—Å
i0Guinier0.0828.2e-051/cm
i0P(r)0.084950.0013071/cm
mwExperimental120.0—kDa
mwGuinier I(0)114.0—kDa
rgGuinier2.820.103Å
rgP(r)3.08—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2OBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2019-04-24Storage / measurement temperature25.0 / 25.0
Exposure time43200.0Frames1
Wavelength0.52Sample-to-detector distance2.0
Sourceneutron sourceDetector1 m long He3 position sensitive detector
Facility / beamlineDhruva Reactor, Bhabha Atomic Research Centre / SANS-I facility · Mumbai, India
q range0.162 – 1.99Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (6 items)
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:182 field values
Field pathRaw value
codeSASDF87
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleSmall-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
project.publication.titleSmall-angle neutron scattering studies suggest the mechanism of BinAB protein internalization
project.publication.author_listSharma M, Aswal V, Kumar V, Chidambaram R
project.publication.journalIUCrJ
project.publication.doi10.1107/S2052252519017159
project.publication.pmidnull
project.publication.published_date2020 Jan 25
project.statusreleased
project.submitted_date2019-06-09
project.released_date2020-02-03
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experiment.instrument.detector.name1 m long He3 position sensitive detector
experiment.instrument.detector.resolution12.0
experiment.instrument.nameDhruva Reactor, Bhabha Atomic Research Centre
experiment.instrument.cityMumbai
experiment.instrument.countryIndia
experiment.instrument.beamline_nameSANS-I facility
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceneutron source
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameBinary toxin receptor Cqm1 protein
experiment.sample.molecule[0].short_nameCqm1
experiment.sample.molecule[0].sequence>VTO94530.1 Cqm1 receptor, mature form (23-560), partial [synthetic gene] (with tag) MHHHHHHSSGVDLGTENLYFQSMREPDSKDWYQHATFYQIYPRSFLDSNGDGIGDLAGITSKMKYLADIGIDA TWLSPPFKSPLKDFGYDVSDFYAIQPEYGNLTDFDKLVEESHKNGIKLMLDFIPNHSSDQHEWFVKSVVRDPE YSDFYVWRPPATGGGPPNNWISVFGGSAWTYNQARGEYYLHQFTPQQPDLNYRNPKVLAEMTKMLFFWLDRGV DGFRLDAINHMFEDEQFRDEPLSGWGQPGEYDSLDHIYTKDIPDVYDVVYNWRDQMDKYSAEKGRTIILMTEA YSSIEGTMLYYESADRKRQGAHMPFNFQLIYDFKKEQNAVGLKSSIDWWMNNMPARHTPSWVAGSHDHSRVAS RVGLDRVDQVMTLLHTLPGTSITYYGEEVAMQDFKEAQQFDNRDPNRTPMQWDSSTSAGFSTNTNTWLRVHPD YARYNVDVMQKNPQSTFHHFQHLTKLRRHRTMQSGEYVHKTVGTKVYALLRELRGEDSFLTVLNMAGAEDTVD LGDFVNLPQKMRVEVAQPNSKSKAGNEVDISKLTLGPYDSVVLRATVSSA
experiment.sample.molecule[0].organismsynthetic construct
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw64.67
experiment.sample.molecule[0].total_mw129.2
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name25 mM HEPES, pH 7.5, 25 mM NaCl, in 100% D2O
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
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experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMosqutio-larvicidal Binary (BinAB) toxin Receptor Cqm1 protein in 100% D2O
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMumbai 400085, INDIA
experiment.contributor[0].affiliation[0].full_nameBhabha Atomic Research Centre
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameVinay
experiment.contributor[0].contributor_surnameKumar
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-7619-6236
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-04-24
experiment.storage_temperature25.0
experiment.cell_temperature25.0
experiment.exposure_time43200.0
experiment.number_of_frames1
experiment.wavelength0.52
experiment.sample_detector_distance2.0
experiment.concentration_minnull
experiment.concentration_max3.3
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fits[1].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.5a
i0_calibration_standardnull
descriptionMolecular weight estimated from experimental data using I(0) value from GNOM analysis. Submitted SANS data has been normalized to protein concentration of 1 mg/ml. Protein (3.33 mg/ml) was used during SANS experiments
experiment_descriptionnull
tags[]
intensity_unit1/cm
experimental_mw120.0
experimental_mw_errornull
guinier_i0_mw114.0
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.08495
pddf_i0_error0.001307
guinier_i00.082
guinier_i0_error8.2e-05
pddf_rg3.08
pddf_rg_errornull
guinier_rg2.82
guinier_rg_error0.103
pddf_dmax9.66
pddf_dmax_errornull
porod_volumenull
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estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last17
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDF87_datloglog_img.png
symmetrynull
last_modified2020-02-03T17:28:44.674498+01:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDF87
statussuccess
started_at2026-08-11T15:24:00.413153+00:00
finished_at2026-08-11T15:24:09.672460+00:00
source_last_modified2020-02-03T17:28:44.674498+01:00
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View the complete summary.json source
{
  "code": "SASDF87",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization",
    "publication": {
      "title": "Small-angle neutron scattering studies suggest the mechanism of BinAB protein internalization",
      "author_list": "Sharma M, Aswal V, Kumar V, Chidambaram R",
      "journal": "IUCrJ",
      "doi": "10.1107/S2052252519017159",
      "pmid": null,
      "published_date": "2020 Jan 25"
    },
    "status": "released",
    "submitted_date": "2019-06-09",
    "released_date": "2020-02-03"
  },
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    "sample": {
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        {
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View the complete manifest.json source
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