Ignicoccus islandicus malate dehydrogenase

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  "project": {
    "title": "The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization",
    "publication": {
      "title": "The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization",
      "author_list": "Roche J, Girard E, Mas C, Madern D",
      "journal": "Journal of Structural Biology",
      "doi": "/10.1016/j.jsb.2019.07.006",
      "pmid": null,
      "published_date": "2019 Jul"
    },
    "status": "released",
    "submitted_date": "2019-02-20",
    "released_date": "2019-07-11"
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  "experiment": {
    "instrument": {
      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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      "beam_profile_al": null
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    "sample": {
      "molecule": [
        {
          "long_name": "Malate dehydrogenase",
          "short_name": null,
          "sequence": "MARIPYKVAVIGTGRVGATFAYTMAVVPGIARMTLVDVVPGLAKGVMEDIKHAAAVFRRSITVEAFEDVSKVENADAIVITAGKPRKADMSRRDLANVNAQIIRDIGDKLRDRNPGALYVVVTNPVDVMTMVLDDVIGSKGTVIGTGTSLDTFRFRAAVSELLNVPIVAVDGYVVGEHGEEAFVAWSTVTIKGIHIDQYIKERNINISREQIEKYVKDVAASIIASQGATIWGPAATFQEIVVSHLANESKIIPISLPQNIEGVGRVAVSVPTIISGRLKPLVQLLNEEEQERLKRAAKAIRNVYESILT",
          "organism": "Ignicoccus islandicus DSM 13165",
          "uniprot_code": "A0A0U3FQH7",
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MARIPYKVAVIGTGRVGATFAYTMAVVPGIARMTLVDVVPGLAKGVMEDIKHAAAVFRRS\nITVEAFEDVSKVENADAIVITAGKPRKADMSRRDLANVNAQIIRDIGDKLRDRNPGALYV\nVVTNPVDVMTMVLDDVIGSKGTVIGTGTSLDTFRFRAAVSELLNVPIVAVDGYVVGEHGE\nEAFVAWSTVTIKGIHIDQYIKERNINISREQIEKYVKDVAASIIASQGATIWGPAATFQE\nIVVSHLANESKIIPISLPQNIEGVGRVAVSVPTIISGRLKPLVQLLNEEEQERLKRAAKA\nIRNVYESILT",
          "mw": 33.552,
          "total_mw": 134.208,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM Tris-HCl 50 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Ignicoccus islandicus malate dehydrogenase",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "IBS",
            "address": "Grenoble, France",
            "full_name": "Institut de Biologie Structurale",
            "webpage": ""
          }
        ],
        "contributor_name": "Jennifer",
        "contributor_surname": "Roche",
        "orcid": "https://orcid.org/0000-0002-6234-1340"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-09-05",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 10,
    "wavelength": 0.125,
    "sample_detector_distance": 2.85,
    "concentration_min": 0.39,
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  "pddf_software_version": "5.0",
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  "experiment_description": "Synchrotron SAXS data from solutions of I. islandicus malate dehydrogenase in 50 mM Tris-HCl 50 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.125 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 9.3 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.",
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  "guinier_point_last": 75,
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  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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