数据类型:SASBDB 实验数据状态:Published曲线类型:Extrapolated to infinite dilution最后更新:2019-12-11T18:17:14.164307+01:00
▼
1. 样品、组分与实验条件 Sample & Experiment
样品 1 · Poly-adenosine rA30 in 600 mM NaCl
浓度
0.4 – 0.8
缓冲液 / pH
1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA / 7.0
实验温度
25.0
设备 / 束线
Cornell High Energy Synchrotron Source (CHESS) / G1
波长
0.11 nm
曝光
10.0 s × 30
分子组分
组分
类型 / 物种
UniProt 与构建体
寡聚状态
分子量
Poly-adenosine查看序列
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
RNA—
——–—
monomer分子数 1
9.894 kDa
实验曲线
曲线
点数 / 列
q 范围
误差质量
负强度点
来源文件
1
261[3]
0.01–0.27 1/A
含误差列缺失 0
0
sasbdb/entries/e9/sasdfe9/source/SASDFE9.dat
▼
2. SASBDB 报告的指标 Reported Results
指标
方法
数值
误差
单位
dmax
P(r)
10.17
—
Å
i0
Guinier
18.38
0.43
—
i0
P(r)
19.11
—
—
mw
Experimental
14.0
—
kDa
porod_volume
Porod
13.0
—
ų
rg
Guinier
2.226
0.05
Å
rg
P(r)
2.47
—
Å
这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。
▼
3. 来源拟合与模型 Source Fits & Models
该条目没有来源拟合记录。
▶
4. 来源文件索引 Source Files
类别
路径
下载状态
大小
curve
sasbdb/entries/e9/sasdfe9/source/SASDFE9.dat
downloaded
5450
full_entry_zip
sasbdb/entries/e9/sasdfe9/source/SASDFE9.zip
downloaded
8959
pddf
sasbdb/entries/e9/sasdfe9/source/SASDFE9.out
downloaded
27015
sascif
sasbdb/entries/e9/sasdfe9/source/SASDFE9.sascif
downloaded
36487
summary
sasbdb/entries/e9/sasdfe9/source/summary.json
downloaded
6017
▶
5. 实验说明与论文 Experiment & Publication
实验说明原文
Synchrotron SAXS data from solutions of rA30 in 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Finger Lakes CCD detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.11 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 0.8 mg/ml were measured at 25°C. 30 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
相关论文
标题
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics.
Synchrotron SAXS data from solutions of rA30 in 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Finger Lakes CCD detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.11 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 0.8 mg/ml were measured at 25°C. 30 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.