| code | SASDFK7 |
| status | Published |
| type_of_curve | Single concentration |
| angular_unit | 1/A |
| project.title | Histone chaperone exploits intrinsic disorder to switch acetylation specificity (Asf1-H3:H4-Rtt109-Vps75 protein complex, data for docking block selections) |
| project.publication.title | Histone chaperone exploits intrinsic disorder to switch acetylation specificity (Asf1-H3:H4-Rtt109-Vps75 protein complex, data for docking block selections) |
| project.publication.author_list | Danilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T |
| project.publication.journal | Nat Commun |
| project.publication.doi | null |
| project.publication.pmid | null |
| project.publication.published_date | 2019 Aug 6 |
| project.status | released |
| project.submitted_date | 2019-06-17 |
| project.released_date | 2019-07-10 |
| pddf_data | https://www.sasbdb.org/media/p_of_R_files/SASDFK7.out |
| intensities_data | https://www.sasbdb.org/media/intensities_files/SASDFK7.dat |
| intensities_log_plot | https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFK7_dat_img.png |
| intensities_kratky_plot | https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFK7_kratky_img.png |
| pddf_plot | https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFK7_pofr_img.png |
| intensities_guinier_plot | https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFK7_guinier_img.png |
| sascif_data | https://www.sasbdb.org/media/sascif/sascif_files/SASDFK7.sascif |
| experiment.instrument.detector.type | 128 linear sensitive multidetector |
| experiment.instrument.detector.name | Reuter-Stokes 3He |
| experiment.instrument.detector.resolution | 0.8 |
| experiment.instrument.name | Institut Laue-Langevin (ILL) |
| experiment.instrument.city | Grenoble |
| experiment.instrument.country | France |
| experiment.instrument.beamline_name | D22 |
| experiment.instrument.beam_geometry | null |
| experiment.instrument.type_of_source | neutron source |
| experiment.instrument.point_source | null |
| experiment.instrument.line_collimation | null |
| experiment.instrument.sample_path_length | null |
| experiment.instrument.line_collimation_slitlength | null |
| experiment.instrument.line_collimation_integrationwidth | null |
| experiment.instrument.xray_energy | null |
| experiment.instrument.beam_profile_ah | null |
| experiment.instrument.beam_profile_al | null |
| experiment.sample.molecule[0].long_name | Vacuolar protein sorting-associated protein 75 (1-225 aa) |
| experiment.sample.molecule[0].short_name | Vps75 1-225 |
| experiment.sample.molecule[0].sequence | MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI
VLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ
VTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF
GWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLED |
| experiment.sample.molecule[0].organism | Saccharomyces cerevisiae |
| experiment.sample.molecule[0].uniprot_code | P53853 |
| experiment.sample.molecule[0].uniprot_range_first | 1 |
| experiment.sample.molecule[0].uniprot_range_last | 225 |
| experiment.sample.molecule[0].oligomerization | dimer |
| experiment.sample.molecule[0].molecular_type | protein |
| experiment.sample.molecule[0].uniprot_sequence | MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI
VLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ
VTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF
GWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSED
DDGSLGEVDLPLSDEEPSSKKRKV |
| experiment.sample.molecule[0].mw | 26.554 |
| experiment.sample.molecule[0].total_mw | 53.108 |
| experiment.sample.molecule[0].number_molecules | 2 |
| experiment.sample.molecule[0].complex_state | False |
| experiment.sample.molecule[0].deuteration | null |
| experiment.sample.molecule[0].molecule_source | biological |
| experiment.sample.molecule[0].molecule_description | null |
| experiment.sample.molecule[1].long_name | Histone acetyltransferase RTT109 |
| experiment.sample.molecule[1].short_name | Rtt109 |
| experiment.sample.molecule[1].sequence | MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK
VFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL
QKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT
CPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA
KLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE
DRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA
IKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA
ITMLKPRKKAKALPKT |
| experiment.sample.molecule[1].organism | Saccharomyces cerevisiae |
| experiment.sample.molecule[1].uniprot_code | Q07794 |
| experiment.sample.molecule[1].uniprot_range_first | 1 |
| experiment.sample.molecule[1].uniprot_range_last | 436 |
| experiment.sample.molecule[1].oligomerization | monomer |
| experiment.sample.molecule[1].molecular_type | protein |
| experiment.sample.molecule[1].uniprot_sequence | MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK
VFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL
QKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT
CPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA
KLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE
DRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA
IKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA
ITMLKPRKKAKALPKT |
| experiment.sample.molecule[1].mw | 50.095 |
| experiment.sample.molecule[1].total_mw | 50.095 |
| experiment.sample.molecule[1].number_molecules | 1 |
| experiment.sample.molecule[1].complex_state | False |
| experiment.sample.molecule[1].deuteration | null |
| experiment.sample.molecule[1].molecule_source | biological |
| experiment.sample.molecule[1].molecule_description | null |
| experiment.sample.molecule[2].long_name | Histone chaperone ASF1 |
| experiment.sample.molecule[2].short_name | Asf1 |
| experiment.sample.molecule[2].sequence | MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI
LVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE
ELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV |
| experiment.sample.molecule[2].organism | null |
| experiment.sample.molecule[2].uniprot_code | P32447 |
| experiment.sample.molecule[2].uniprot_range_first | 1 |
| experiment.sample.molecule[2].uniprot_range_last | 169 |
| experiment.sample.molecule[2].oligomerization | monomer |
| experiment.sample.molecule[2].molecular_type | protein |
| experiment.sample.molecule[2].uniprot_sequence | MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI
LVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE
ELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE
DDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE
EEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN |
| experiment.sample.molecule[2].mw | 19.125 |
| experiment.sample.molecule[2].total_mw | 19.125 |
| experiment.sample.molecule[2].number_molecules | 1 |
| experiment.sample.molecule[2].complex_state | False |
| experiment.sample.molecule[2].deuteration | null |
| experiment.sample.molecule[2].molecule_source | biological |
| experiment.sample.molecule[2].molecule_description | null |
| experiment.sample.molecule[3].long_name | Histone H3.2 (35-135 aa) |
| experiment.sample.molecule[3].short_name | H3 (35-135) |
| experiment.sample.molecule[3].sequence | VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAY
LVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA |
| experiment.sample.molecule[3].organism | Xenopus laevis |
| experiment.sample.molecule[3].uniprot_code | P84233 |
| experiment.sample.molecule[3].uniprot_range_first | 35 |
| experiment.sample.molecule[3].uniprot_range_last | 135 |
| experiment.sample.molecule[3].oligomerization | monomer |
| experiment.sample.molecule[3].molecular_type | protein |
| experiment.sample.molecule[3].uniprot_sequence | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE
LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI
MPKDIQLARRIRGERA |
| experiment.sample.molecule[3].mw | 11.747 |
| experiment.sample.molecule[3].total_mw | 11.747 |
| experiment.sample.molecule[3].number_molecules | 1 |
| experiment.sample.molecule[3].complex_state | False |
| experiment.sample.molecule[3].deuteration | null |
| experiment.sample.molecule[3].molecule_source | biological |
| experiment.sample.molecule[3].molecule_description | null |
| experiment.sample.molecule[4].long_name | Histone H4 |
| experiment.sample.molecule[4].short_name | H4 |
| experiment.sample.molecule[4].sequence | MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG |
| experiment.sample.molecule[4].organism | Xenopus laevis |
| experiment.sample.molecule[4].uniprot_code | P62799 |
| experiment.sample.molecule[4].uniprot_range_first | 1 |
| experiment.sample.molecule[4].uniprot_range_last | 103 |
| experiment.sample.molecule[4].oligomerization | monomer |
| experiment.sample.molecule[4].molecular_type | protein |
| experiment.sample.molecule[4].uniprot_sequence | MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK
VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG |
| experiment.sample.molecule[4].mw | 11.367 |
| experiment.sample.molecule[4].total_mw | 11.367 |
| experiment.sample.molecule[4].number_molecules | 1 |
| experiment.sample.molecule[4].complex_state | False |
| experiment.sample.molecule[4].deuteration | null |
| experiment.sample.molecule[4].molecule_source | biological |
| experiment.sample.molecule[4].molecule_description | null |
| experiment.sample.buffer.name | 50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O |
| experiment.sample.buffer.concentration_unit | null |
| experiment.sample.buffer.comment | null |
| experiment.sample.buffer.additive | null |
| experiment.sample.buffer.concentration | null |
| experiment.sample.buffer.pka | null |
| experiment.sample.buffer.ph | 6.5 |
| experiment.sample.buffer.deuteration | null |
| experiment.sample.purity_method | null |
| experiment.sample.name | Complex with 1H histone chaperone Asf1, histones H3 (35-135aa) and H4; 70%-2H histone acetyltransferase Rtt109 and histone chaperone Vps75 (1-225aa) acquired in 100% v/v D2O |
| experiment.sample.ext_coefficient | null |
| experiment.sample.contrast | null |
| experiment.sample.specific_vol | null |
| experiment.sample.dry_vol | null |
| experiment.sample.absorbption | null |
| experiment.sample.deuteration | null |
| experiment.sample.mixture | null |
| experiment.contributor[0].affiliation[0].short_name | null |
| experiment.contributor[0].affiliation[0].address | Germany |
| experiment.contributor[0].affiliation[0].full_name | Leibniz University of Hannover |
| experiment.contributor[0].affiliation[0].webpage | https://www.uni-hannover.de |
| experiment.contributor[0].contributor_name | Nataliya |
| experiment.contributor[0].contributor_surname | Danilenko |
| experiment.contributor[0].orcid | https://orcid.org/0000-0003-4885-5051 |
| experiment.concentration_method | null |
| experiment.concentration_unit | null |
| experiment.date | 2016-11-14 |
| experiment.storage_temperature | null |
| experiment.cell_temperature | 25.1 |
| experiment.exposure_time | 3600.0 |
| experiment.number_of_frames | 1 |
| experiment.wavelength | 0.6 |
| experiment.sample_detector_distance | 4.0 |
| experiment.concentration_min | null |
| experiment.concentration_max | 2.55 |
| experiment.sample_volume | null |
| experiment.flow_rate | null |
| experiment.s_min | 0.122 |
| experiment.s_max | 2.405 |
| experiment.total_exposure_time | null |
| experiment.seccolumn | null |
| fits[0].models[0].model_plot | https://www.sasbdb.org/media/pdb_file/images/SASDFK7_fit1_model1_img.png |
| fits[0].models[0].software | HADDOCK |
| fits[0].models[0].pdb_link | [] |
| fits[0].models[0].model_title | null |
| fits[0].models[0].type_of_model | atomic |
| fits[0].models[0].software_version | null |
| fits[0].models[0].model_data | https://www.sasbdb.org/media/pdb_file/SASDFK7_fit1_model1.pdb |
| fits[0].models[0].model_mw | 144.0 |
| fits[0].models[0].bead_radius | null |
| fits[0].models[0].log | null |
| fits[0].models[0].symmetry | null |
| fits[0].models[0].comment | null |
| fits[0].models[0].user | 420 |
| fits[0].fit_unit | 1/A |
| fits[0].fit_plot | https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFK7_fit1_fixed_fit_img.png |
| fits[0].software | CRYSON |
| fits[0].chi_square_value | 0.696 |
| fits[0].p_value | 0.0 |
| fits[0].fit_residual_plot | fit4columns2379_fitresiduals_img.png |
| fits[0].fit_data | https://www.sasbdb.org/media/fitting_files/SASDFK7_fit1.fit |
| fits[0].fit_log | https://www.sasbdb.org/media/fitting_files/log_files/new190718_12000_M6XGXbc.log |
| fits[0].software_version | 2.7.1 |
| fits[0].description | |
| estimated_volume_method | null |
| pddf_software | ATSAS GNOM |
| pddf_software_version | 4.6 |
| i0_calibration_standard | null |
| description | null |
| experiment_description | SANS data from solutions the Asf1-H3:H4-Rtt109-Vps75 protein complex (1H Asf1-H3(35-135):H4, bound to 70% 2H-labelled Rtt109-Vps75(1-225)) in 50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O, pD 6.5 were collected on the D22 instrument at the ILL (Grenoble, France) using a 3He multidetector 128 linear sensitive Reuter-Stokes detector detector at a sample-detector/Collimation distance of 4 m/4 m and at a wavelength of λ = 0.6 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.55 mg/ml was measured at 25°C for 1 h. |
| tags | [] |
| intensity_unit | arbitrary |
| experimental_mw | 145.0 |
| experimental_mw_error | null |
| guinier_i0_mw | null |
| guinier_i0_mw_error | null |
| porod_mw | null |
| porod_mw_error | null |
| pddf_i0 | 0.02038 |
| pddf_i0_error | null |
| guinier_i0 | 0.02 |
| guinier_i0_error | 0.00022 |
| pddf_rg | 2.06 |
| pddf_rg_error | null |
| guinier_rg | 1.943 |
| guinier_rg_error | 0.036 |
| pddf_dmax | 5.5 |
| pddf_dmax_error | null |
| porod_volume | null |
| porod_volume_error | null |
| estimated_volume | null |
| estimated_volume_error | null |
| guinier_point_first | 1 |
| guinier_point_last | 27 |
| pddf_point_first | null |
| pddf_point_last | null |
| i0_calibration_standard_data | null |
| intensities_log_log_plot | SASDFK7_datloglog_img.png |
| symmetry | null |
| last_modified | 2022-08-15T14:51:04.900446+02:00 |
| bragg_peak | [] |