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{
"code": "SASDFM3",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/A",
"project": {
"title": "Histone chaperone exploits intrinsic disorder to switch acetylation specificity.",
"publication": {
"title": "Histone chaperone exploits intrinsic disorder to switch acetylation specificity.",
"author_list": "Danilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T",
"journal": "Nat Commun",
"doi": "10.1038/s41467-019-11410-7",
"pmid": "31387991",
"published_date": "2019 Aug 6"
},
"status": "released",
"submitted_date": "2019-02-27",
"released_date": "2019-07-10"
},
"pddf_data": null,
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFM3.dat",
"intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM3_dat_img.png",
"intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM3_kratky_img.png",
"pddf_plot": null,
"intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM3_guinier_img.png",
"sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFM3.sascif",
"experiment": {
"instrument": {
"detector": {
"type": "SANS",
"name": "6Li-Scintillator 1 mm thickness + photomultiplier",
"resolution": 5.3
},
"name": "FRM2",
"city": "Munich",
"country": "Germany",
"beamline_name": "KWS1",
"beam_geometry": null,
"type_of_source": "neutron source",
"point_source": null,
"line_collimation": null,
"sample_path_length": null,
"line_collimation_slitlength": null,
"line_collimation_integrationwidth": null,
"xray_energy": null,
"beam_profile_ah": null,
"beam_profile_al": null
},
"sample": {
"molecule": [
{
"long_name": "Vacuolar protein sorting-associated protein 75 (1-225 aa)",
"short_name": "Vps75 1-225",
"sequence": "MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\r\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\r\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\r\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLED",
"organism": "Saccharomyces cerevisiae",
"uniprot_code": "P53853",
"uniprot_range_first": 1,
"uniprot_range_last": 225,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSED\nDDGSLGEVDLPLSDEEPSSKKRKV",
"mw": 26.554,
"total_mw": 53.108,
"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Histone acetyltransferase RTT109",
"short_name": "Rtt109",
"sequence": "MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK\r\nVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL\r\nQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT\r\nCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA\r\nKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE\r\nDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA\r\nIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA\r\nITMLKPRKKAKALPKT",
"organism": "Saccharomyces cerevisiae",
"uniprot_code": "Q07794",
"uniprot_range_first": 1,
"uniprot_range_last": 436,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK\nVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL\nQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT\nCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA\nKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE\nDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA\nIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA\nITMLKPRKKAKALPKT",
"mw": 50.095,
"total_mw": 50.095,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Histone chaperone ASF1",
"short_name": "Asf1",
"sequence": "MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\r\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\r\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV",
"organism": null,
"uniprot_code": "P32447",
"uniprot_range_first": 1,
"uniprot_range_last": 169,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE\nDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE\nEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN",
"mw": 19.125,
"total_mw": 19.125,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Histone H3.2 (35-135 aa)",
"short_name": "H3 (35-135)",
"sequence": "VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAY \r\nLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA",
"organism": "Xenopus laevis",
"uniprot_code": "P84233",
"uniprot_range_first": 35,
"uniprot_range_last": 135,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE\nLLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI\nMPKDIQLARRIRGERA",
"mw": 11.747,
"total_mw": 11.747,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
},
{
"long_name": "Histone H4",
"short_name": "H4",
"sequence": "MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\r\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG",
"organism": "Xenopus laevis",
"uniprot_code": "P62799",
"uniprot_range_first": 1,
"uniprot_range_last": 103,
"oligomerization": "monomer",
"molecular_type": "protein",
"uniprot_sequence": "MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG",
"mw": 11.367,
"total_mw": 11.367,
"number_molecules": 1,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": null
}
],
"buffer": {
"name": "50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O",
"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 6.5,
"deuteration": null
},
"purity_method": null,
"name": "Complex with 1H histone chaperone Asf1 and histones H3 and H4, 2H histone acetyltransferase Rtt109 and histone chaperone Vps75 (1H Asf1-H3:H4, 2H Rtt109-Vps75) acquired in 100% v/v D2O",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Germany",
"full_name": "Leibniz University of Hannover",
"webpage": "https://www.uni-hannover.de"
}
],
"contributor_name": "Nataliya",
"contributor_surname": "Danilenko",
"orcid": "https://orcid.org/0000-0003-4885-5051"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2017-03-04",
"storage_temperature": 25.0,
"cell_temperature": 25.0,
"exposure_time": 21600.0,
"number_of_frames": null,
"wavelength": 0.5,
"sample_detector_distance": 4.0,
"concentration_min": 3.85,
"concentration_max": 3.85,
"sample_volume": null,
"flow_rate": null,
"s_min": 0.127,
"s_max": 3.455,
"total_exposure_time": null,
"seccolumn": null
},
"fits": [
{
"models": [
{
"model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFM3_fit1_model1_img.png",
"software": "HADDOCK",
"pdb_link": [
{
"pdb_code": "6o22",
"ext_code": "6O22",
"ext_type": "pdb",
"difference_with_model": "derived"
}
],
"model_title": null,
"type_of_model": "atomic",
"software_version": null,
"model_data": "https://www.sasbdb.org/media/pdb_file/SASDFM3_fit1_model1.pdb",
"model_mw": 144.0,
"bead_radius": null,
"log": "https://www.sasbdb.org/media/log_files/new190718_12003.log",
"symmetry": null,
"comment": null,
"user": 420
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/new190718_12003_fit_img.png",
"software": "CRYSON",
"chi_square_value": 15.132,
"p_value": 0.0,
"fit_residual_plot": null,
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFM3_fit1.fit",
"fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/new190718_12003.log",
"software_version": null,
"description": ""
}
],
"estimated_volume_method": null,
"pddf_software": null,
"pddf_software_version": null,
"i0_calibration_standard": null,
"description": null,
"experiment_description": "SANS data from solutions of the Asf1-H3:H4-Rtt109-Vps75 protein complex (protonated Asf1-H3:H4 bound to perdeuterated Rtt109-Vps75) in 50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O, pD 6.5 were collected on the KWS1 instrument at FRM2 (Munich, Germany) using a SANS 6Li-Scintillator 1 mm thickness + photomultiplier detector. Due to a combination of negative (from 1H Asf1-H3:H4) and positive (from perdeuterated Rtt109-Vps75) contrasts the Rg obtained from the Guinier approximation is negative. The data were recorded at two detector configurations: 1) 4 m for 6 hrs using a neutron wavelength of 0.5 nm and; 2) 1.5 m for 2.5 hrs using a neutron wavelength of 0.5 nm. Both measurement were performed on the same sample at 3.85 mg/mL, measured at 25°C.",
"tags": [],
"intensity_unit": null,
"experimental_mw": 145.0,
"experimental_mw_error": null,
"guinier_i0_mw": null,
"guinier_i0_mw_error": null,
"porod_mw": null,
"porod_mw_error": null,
"pddf_i0": null,
"pddf_i0_error": null,
"guinier_i0": 0.017,
"guinier_i0_error": 0.0063,
"pddf_rg": null,
"pddf_rg_error": null,
"guinier_rg": -2.81,
"guinier_rg_error": 0.327,
"pddf_dmax": null,
"pddf_dmax_error": null,
"porod_volume": null,
"porod_volume_error": null,
"estimated_volume": null,
"estimated_volume_error": null,
"guinier_point_first": 3,
"guinier_point_last": 10,
"pddf_point_first": null,
"pddf_point_last": null,
"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDFM3_datloglog_img.png",
"symmetry": null,
"last_modified": "2019-12-06T13:07:15.624532+01:00",
"bragg_peak": []
}
