查看完整 summary.json 原文
{
"code": "SASDFN2",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.",
"publication": {
"title": "Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.",
"author_list": "Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B",
"journal": "J Struct Biol",
"doi": "10.1016/j.jsb.2019.107429",
"pmid": "31778770",
"published_date": "2019 Nov 25"
},
"status": "released",
"submitted_date": "2019-01-30",
"released_date": "2020-01-09"
},
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"name": "Rayonix MX225-HE",
"resolution": null
},
"name": "Stanford Synchrotron Radiation Lightsource (SSRL)",
"city": "Menlo Park, CA",
"country": "USA",
"beamline_name": "BL4-2",
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"type_of_source": "X-ray synchrotron",
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"sample": {
"molecule": [
{
"long_name": "Cell wall synthesis protein Wag31",
"short_name": null,
"sequence": "MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL\r\nAAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT\r\nAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA\r\nDALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA\r\nAPVDSNADAGGFDQFNRGKN",
"organism": "Mycobacterium tuberculosis",
"uniprot_code": "P9WMU1",
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"oligomerization": "unknown",
"molecular_type": "protein",
"uniprot_sequence": "MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL\nAAGGGAGVTPQATQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT\nAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA\nDALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA\nAPVDSNADAGGFDQFNRGKN",
"mw": 29.98,
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"number_molecules": 0,
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"deuteration": null,
"molecule_source": "biological",
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}
],
"buffer": {
"name": "50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)",
"concentration_unit": null,
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"additive": null,
"concentration": null,
"pka": null,
"ph": 7.5,
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},
"purity_method": null,
"name": "Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 280-284)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": "CSIR",
"address": "Chandigarh, India",
"full_name": "CSIR-Institute of Microbial Technology",
"webpage": "http://www.imtech.res.in/"
}
],
"contributor_name": "Komal",
"contributor_surname": "Choukate",
"orcid": ""
}
],
"concentration_method": null,
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"date": "2018-01-29",
"storage_temperature": 23.0,
"cell_temperature": 23.0,
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"wavelength": 0.1,
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"description": "SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak.\r\nSEC-SAXS data of Cell wall synthesis protein Wag31(T73E) polymer frame no. 280-284",
"experiment_description": "Synchrotron SAXS data from solutions of Cell wall synthesis protein Wag31(T73E) polymer (SEC-frames 280-284) in 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 23°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
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"intensities_log_log_plot": "SASDFN2_datloglog_img.png",
"symmetry": null,
"last_modified": "2020-08-13T15:34:09.828154+02:00",
"bragg_peak": []
}
查看完整 manifest.json 原文
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