Domain-stapled mutant of Matrix metalloproteinase-1 (MMP-1 S243C, S318C)

{
  "code": "SASDFN4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Conformationally constrained mutant of human matrix metalloproteinase-1",
    "publication": null,
    "status": "released",
    "submitted_date": "2019-03-26",
    "released_date": "2021-09-27"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFN4.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFN4.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFN4_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFN4_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFN4_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFN4_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFN4.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": "1 x 5 mm",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Matrix metalloproteinase-1 (Interstitial collagenase) (S243C, S318C)",
          "short_name": "MMP-1 (S243C, S318C)",
          "sequence": "FVLTEGNPRWEQTHLTYRIEN\r\nYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGN\r\nLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSY\r\nTFCGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDR\r\nFYMRTNPFYPEVELNFICVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGY\r\nPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFP\r\nGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN",
          "organism": "Homo sapiens",
          "uniprot_code": "P03956",
          "uniprot_range_first": 100,
          "uniprot_range_last": 469,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPV\nVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIEN\nYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGN\nLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSY\nTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDR\nFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGY\nPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFP\nGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN",
          "mw": 42.666,
          "total_mw": 42.666,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Domain-stapled mutant of Matrix metalloproteinase-1 (MMP-1 S243C, S318C)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Portsmouth, UK",
            "full_name": "University of Portsmouth",
            "webpage": "https://www.port.ac.uk"
          }
        ],
        "contributor_name": "Rob",
        "contributor_surname": "Holland",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2016-05-14",
    "storage_temperature": 4.0,
    "cell_temperature": 25.0,
    "exposure_time": 3.0,
    "number_of_frames": 29,
    "wavelength": 0.1,
    "sample_detector_distance": 3.9,
    "concentration_min": 0.0,
    "concentration_max": 8.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.04,
    "s_max": 4.078,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of the domain-stapled mutant of matrix metalloproteinase-1 (MMP-1 S243C, S318C) in 50 mM Tris-HCl, 150 mM NaCl, 10 mM CaCl2, pH 7.4 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25 °C using the following parameters: Shodex Kw-403 analytical SEC column; Flow rate: 0.2 mL/min; Sample injection concentration: 8 mg/mL; Injection volume: 45 μL. The data were collected through the SEC peak of the sample as a series of 29 x 3 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 33.825,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 29.0,
  "porod_mw_error": null,
  "pddf_i0": 0.001541,
  "pddf_i0_error": 0.0001,
  "guinier_i0": 0.001574,
  "guinier_i0_error": 1.2e-05,
  "pddf_rg": 2.484,
  "pddf_rg_error": 0.02,
  "guinier_rg": 2.562,
  "guinier_rg_error": 0.033,
  "pddf_dmax": 7.74,
  "pddf_dmax_error": null,
  "porod_volume": 43.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 21,
  "guinier_point_last": 169,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFN4_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2021-09-27T16:14:08.122509+02:00",
  "bragg_peak": []
}

{
  "code": "SASDFN4",
  "status": "success",
  "started_at": "2026-08-11T14:27:19.507455+00:00",
  "finished_at": "2026-08-11T14:27:27.704075+00:00",
  "source_last_modified": "2021-09-27T16:14:08.122509+02:00",
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