Wild type 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, ACMSD tetramer, at pH 7.0

{
  "code": "SASDFN5",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity.",
    "publication": {
      "title": "Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity.",
      "author_list": "Yang Y, Davis I, Matsui T, Rubalcava I, Liu A",
      "journal": "J Biol Chem",
      "doi": "10.1074/jbc.RA119.009035",
      "pmid": "31189654",
      "published_date": "2019 Jul 26"
    },
    "status": "released",
    "submitted_date": "2019-04-23",
    "released_date": "2019-06-19"
  },
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  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFN5.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFN5_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFN5_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFN5_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFN5_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFN5.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 X 1M",
        "resolution": 0.172
      },
      "name": "Stanford Synchrotron Radiation Lightsource (SSRL)",
      "city": "Menlo Park, CA",
      "country": "USA",
      "beamline_name": "BL4-2",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
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      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "2-amino-3-carboxymuconate 6-semialdehyde decarboxylase",
          "short_name": "ACMSD",
          "sequence": "MGHHHHHHHHHHSSGHIEGRH\r\nMKKPRIDMHSHFFPRISEQEAAKFDANHAPWLQVSAKGDTGSIMMGKNNFRPVYQALWDPAFRIEEMDAQGVDVQVTCAT\r\nPVMFGYTWEANKAAQWAERMNDFALEFAAHNPQRIKVLAQVPLQDLDLACKEASRAVAAGHLGIQIGNHLGDKDLDDATL\r\nEAFLTHCANEDIPILVHPWDMMGGQRMKKWMLPWLVAMPAETQLAILSLILSGAFERIPKSLKICFGHGGGSFAFLLGRV\r\nDNAWRHRDIVREDCPRPPSEYVDRFFVDSAVFNPGALELLVSVMGEDRVMLGSDYPFPLGEQKIGGLVLSSNLGESAKDK\r\nIISGNASKFFNINV",
          "organism": "Pseudomonas fluorescens",
          "uniprot_code": "Q83V25",
          "uniprot_range_first": 1,
          "uniprot_range_last": 334,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MKKPRIDMHSHFFPRISEQEAAKFDANHAPWLQVSAKGDTGSIMMGKNNFRPVYQALWDP\nAFRIEEMDAQGVDVQVTCATPVMFGYTWEANKAAQWAERMNDFALEFAAHNPQRIKVLAQ\nVPLQDLDLACKEASRAVAAGHLGIQIGNHLGDKDLDDATLEAFLTHCANEDIPILVHPWD\nMMGGQRMKKWMLPWLVAMPAETQLAILSLILSGAFERIPKSLKICFGHGGGSFAFLLGRV\nDNAWRHRDIVREDCPRPPSEYVDRFFVDSAVFNPGALELLVSVMGEDRVMLGSDYPFPLG\nEQKIGGLVLSSNLGESAKDKIISGNASKFFNINV",
          "mw": 39.661,
          "total_mw": 158.644,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "25 mM HEPES, 5 mM DTT",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Wild type 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, ACMSD tetramer, at pH 7.0",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "San Antonia, TX, USA",
            "full_name": "University of Texas at San Antonio",
            "webpage": "https://www.utsa.edu/"
          }
        ],
        "contributor_name": "Yu",
        "contributor_surname": "Yang",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-01-10",
    "storage_temperature": 25.0,
    "cell_temperature": 25.0,
    "exposure_time": 1.0,
    "number_of_frames": 500,
    "wavelength": 0.1088,
    "sample_detector_distance": 1.7,
    "concentration_min": null,
    "concentration_max": null,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.151,
    "s_max": 3.092,
    "total_exposure_time": null,
    "seccolumn": 2
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      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/05-P1_Tetra_fixed_fit_img.png",
      "software": "CORAL",
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of wild type ACMSD tetramer in 25 mM HEPES, 5 mM DTT, pH 7 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1088 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed at 25°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 122.0,
  "porod_mw_error": null,
  "pddf_i0": 51.45,
  "pddf_i0_error": 0.83,
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  "guinier_i0_error": 0.89,
  "pddf_rg": 4.83,
  "pddf_rg_error": 0.1,
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  "guinier_rg_error": 0.11,
  "pddf_dmax": 17.5,
  "pddf_dmax_error": null,
  "porod_volume": 195.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 23,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFN5_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}

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