SASDFQ3

Complex with 1H histone acetyltransferase Rtt109 and histones H3 and H4, 2H histone chaperones Asf1 and Vps75 (1H Rtt109-H3:H4, 2H Asf1-Vps75) acquired in 42% v/v D2O

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2019-12-06T13:07:15.624532+01:00
▼

1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Complex with 1H histone acetyltransferase Rtt109 and histones H3 and H4, 2H histone chaperones Asf1 and Vps75 (1H Rtt109-H3:H4, 2H Asf1-Vps75) acquired in 42% v/v D2O

浓度— – 4.7 缓冲液 / pH50 mM citrate, 150 mM NaCl, 5 mM BME, 42% D2O / 6.5
实验温度25.0 设备 / 束线FRM2 / KWS1
波长0.5 nm曝光7200.0 s × 1

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Vacuolar protein sorting-associated protein 75 (1-225 aa)
查看序列
MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKIVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIFGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLED
proteinSaccharomyces cerevisiaeP538531–225dimer分子数 226.554 kDa
Histone acetyltransferase RTT109
查看序列
MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGKVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYLQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFTCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQAKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEEDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRAIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLAITMLKPRKKAKALPKT
proteinSaccharomyces cerevisiaeQ077941–436monomer分子数 150.095 kDa
Histone chaperone ASF1
查看序列
MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV
protein—P324471–169monomer分子数 119.125 kDa
Histone H3.2 (35-135 aa)
查看序列
VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
proteinXenopus laevisP8423335–135monomer分子数 111.747 kDa
Histone H4
查看序列
MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
proteinXenopus laevisP627991–103monomer分子数 111.367 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
195[3]0.01265196–0.3922764 1/A含误差列缺失 00sasbdb/entries/q3/sasdfq3/source/SASDFQ3.dat
▼

2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)11.0—Å
i0Guinier0.250.00092—
i0P(r)0.2503——
mwExperimental146.0—kDa
rgGuinier3.4990.046Å
rgP(r)3.66—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

▼

3. 来源拟合与模型 Source Fits & Models

▶

4. 来源文件索引 Source Files

▶

5. 实验说明与论文 Experiment & Publication

▼

6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM citrate, 150 mM NaCl, 5 mM BME, 42% D2O缓冲液浓度—
pH6.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2017-03-05储存 / 测量温度25.0 / 25.0
曝光时间7200.0帧数1
波长0.5样品-探测器距离4.0
光源neutron source探测器6Li-Scintillator 1 mm thickness + photomultiplier
机构 / 束线FRM2 / KWS1 · Munich, Germany
q 范围0.127 – 3.923样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/q3/sasdfq3/source/SASDFQ3.datdownloaded45614874874d6a6e2355f632372577c071100ab2b7a69f94decb77244008c01eaef8下载查看原文件源站
full_entry_zipsasbdb/entries/q3/sasdfq3/source/SASDFQ3.zipdownloaded20507951cfd36ebd1e4c46e4bceb3210ada84085599a2b9d7c3a69983876bc3e6f687b下载查看原文件源站
pddfsasbdb/entries/q3/sasdfq3/source/SASDFQ3.outdownloaded12893b180bf6b601da79530ffbf8358ab6ffbaee406566f1177bfe37e0497d422fb2a下载查看原文件源站
sascifsasbdb/entries/q3/sasdfq3/source/SASDFQ3.sascifdownloaded1292487be52aa345fc87cf83641c3edd0cc1daa416df049ce4a72671f41fa91da2b4383下载查看原文件源站
summarysasbdb/entries/q3/sasdfq3/source/summary.jsondownloaded12095bce670cdaba871932179fcdee95562026097870db81199e7afef25f5eeac875b下载查看原文件源站
curve:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.dat",
  "role": "curve",
  "sha256": "4874874d6a6e2355f632372577c071100ab2b7a69f94decb77244008c01eaef8",
  "size": 4561,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDFQ3.dat"
}
full_entry_zip:来源记录与 ZIP 内部目录(4 项)
{
  "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.zip",
  "role": "full_entry_zip",
  "sha256": "51cfd36ebd1e4c46e4bceb3210ada84085599a2b9d7c3a69983876bc3e6f687b",
  "size": 205079,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDFQ3.zip",
  "zip_member_count": 4,
  "zip_members": [
    {
      "crc32": "6a01c6c3",
      "name": "SASDFQ3/experimental_data/SASDFQ3.dat",
      "size": 4561
    },
    {
      "crc32": "a51dd535",
      "name": "SASDFQ3/pddf/SASDFQ3.out",
      "size": 12893
    },
    {
      "crc32": "38f9d522",
      "name": "SASDFQ3/SASDFQ3_fit1.fit",
      "size": 3003
    },
    {
      "crc32": "1a1ada18",
      "name": "SASDFQ3/SASDFQ3_fit1_model1.pdb",
      "size": 919380
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.out",
  "role": "pddf",
  "sha256": "b180bf6b601da79530ffbf8358ab6ffbaee406566f1177bfe37e0497d422fb2a",
  "size": 12893,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDFQ3.out"
}
sascif:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.sascif",
  "role": "sascif",
  "sha256": "be52aa345fc87cf83641c3edd0cc1daa416df049ce4a72671f41fa91da2b4383",
  "size": 1292487,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFQ3.sascif"
}
summary:来源记录
{
  "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/summary.json",
  "sha256": "bce670cdaba871932179fcdee95562026097870db81199e7afef25f5eeac875b",
  "size": 12095,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDFQ3/"
}

全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:227 个字段值
字段路径原始值
codeSASDFQ3
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleHistone chaperone exploits intrinsic disorder to switch acetylation specificity.
project.publication.titleHistone chaperone exploits intrinsic disorder to switch acetylation specificity.
project.publication.author_listDanilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-019-11410-7
project.publication.pmid31387991
project.publication.published_date2019 Aug 6
project.statusreleased
project.submitted_date2019-02-27
project.released_date2019-07-10
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDFQ3.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDFQ3.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ3_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ3_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFQ3_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ3_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDFQ3.sascif
experiment.instrument.detector.typeSANS
experiment.instrument.detector.name6Li-Scintillator 1 mm thickness + photomultiplier
experiment.instrument.detector.resolution5.3
experiment.instrument.nameFRM2
experiment.instrument.cityMunich
experiment.instrument.countryGermany
experiment.instrument.beamline_nameKWS1
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceneutron source
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameVacuolar protein sorting-associated protein 75 (1-225 aa)
experiment.sample.molecule[0].short_nameVps75 1-225
experiment.sample.molecule[0].sequenceMMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI VLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ VTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF GWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLED
experiment.sample.molecule[0].organismSaccharomyces cerevisiae
experiment.sample.molecule[0].uniprot_codeP53853
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last225
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI VLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ VTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF GWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSED DDGSLGEVDLPLSDEEPSSKKRKV
experiment.sample.molecule[0].mw26.554
experiment.sample.molecule[0].total_mw53.108
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameHistone acetyltransferase RTT109
experiment.sample.molecule[1].short_nameRtt109
experiment.sample.molecule[1].sequenceMSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK VFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL QKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT CPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA KLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE DRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA IKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA ITMLKPRKKAKALPKT
experiment.sample.molecule[1].organismSaccharomyces cerevisiae
experiment.sample.molecule[1].uniprot_codeQ07794
experiment.sample.molecule[1].uniprot_range_first1
experiment.sample.molecule[1].uniprot_range_last436
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK VFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL QKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT CPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA KLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE DRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA IKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA ITMLKPRKKAKALPKT
experiment.sample.molecule[1].mw50.095
experiment.sample.molecule[1].total_mw50.095
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.molecule[2].long_nameHistone chaperone ASF1
experiment.sample.molecule[2].short_nameAsf1
experiment.sample.molecule[2].sequenceMSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI LVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE ELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV
experiment.sample.molecule[2].organismnull
experiment.sample.molecule[2].uniprot_codeP32447
experiment.sample.molecule[2].uniprot_range_first1
experiment.sample.molecule[2].uniprot_range_last169
experiment.sample.molecule[2].oligomerizationmonomer
experiment.sample.molecule[2].molecular_typeprotein
experiment.sample.molecule[2].uniprot_sequenceMSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI LVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE ELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE DDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE EEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN
experiment.sample.molecule[2].mw19.125
experiment.sample.molecule[2].total_mw19.125
experiment.sample.molecule[2].number_molecules1
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_descriptionnull
experiment.sample.molecule[3].long_nameHistone H3.2 (35-135 aa)
experiment.sample.molecule[3].short_nameH3 (35-135)
experiment.sample.molecule[3].sequenceVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAY LVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
experiment.sample.molecule[3].organismXenopus laevis
experiment.sample.molecule[3].uniprot_codeP84233
experiment.sample.molecule[3].uniprot_range_first35
experiment.sample.molecule[3].uniprot_range_last135
experiment.sample.molecule[3].oligomerizationmonomer
experiment.sample.molecule[3].molecular_typeprotein
experiment.sample.molecule[3].uniprot_sequenceMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE LLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI MPKDIQLARRIRGERA
experiment.sample.molecule[3].mw11.747
experiment.sample.molecule[3].total_mw11.747
experiment.sample.molecule[3].number_molecules1
experiment.sample.molecule[3].complex_stateFalse
experiment.sample.molecule[3].deuterationnull
experiment.sample.molecule[3].molecule_sourcebiological
experiment.sample.molecule[3].molecule_descriptionnull
experiment.sample.molecule[4].long_nameHistone H4
experiment.sample.molecule[4].short_nameH4
experiment.sample.molecule[4].sequenceMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
experiment.sample.molecule[4].organismXenopus laevis
experiment.sample.molecule[4].uniprot_codeP62799
experiment.sample.molecule[4].uniprot_range_first1
experiment.sample.molecule[4].uniprot_range_last103
experiment.sample.molecule[4].oligomerizationmonomer
experiment.sample.molecule[4].molecular_typeprotein
experiment.sample.molecule[4].uniprot_sequenceMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK VFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG
experiment.sample.molecule[4].mw11.367
experiment.sample.molecule[4].total_mw11.367
experiment.sample.molecule[4].number_molecules1
experiment.sample.molecule[4].complex_stateFalse
experiment.sample.molecule[4].deuterationnull
experiment.sample.molecule[4].molecule_sourcebiological
experiment.sample.molecule[4].molecule_descriptionnull
experiment.sample.buffer.name50 mM citrate, 150 mM NaCl, 5 mM BME, 42% D2O
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph6.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameComplex with 1H histone acetyltransferase Rtt109 and histones H3 and H4, 2H histone chaperones Asf1 and Vps75 (1H Rtt109-H3:H4, 2H Asf1-Vps75) acquired in 42% v/v D2O
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressGermany
experiment.contributor[0].affiliation[0].full_nameLeibniz University of Hannover
experiment.contributor[0].affiliation[0].webpagehttps://www.uni-hannover.de
experiment.contributor[0].contributor_nameNataliya
experiment.contributor[0].contributor_surnameDanilenko
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-4885-5051
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2017-03-05
experiment.storage_temperature25.0
experiment.cell_temperature25.0
experiment.exposure_time7200.0
experiment.number_of_frames1
experiment.wavelength0.5
experiment.sample_detector_distance4.0
experiment.concentration_minnull
experiment.concentration_max4.7
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.127
experiment.s_max3.923
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDFQ3_fit1_model1_img.png
fits[0].models[0].softwareHADDOCK
fits[0].models[0].pdb_link[0].pdb_code6o22
fits[0].models[0].pdb_link[0].ext_code6O22
fits[0].models[0].pdb_link[0].ext_typepdb
fits[0].models[0].pdb_link[0].difference_with_modelderived
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDFQ3_fit1_model1.pdb
fits[0].models[0].model_mw144.0
fits[0].models[0].bead_radiusnull
fits[0].models[0].loghttps://www.sasbdb.org/media/log_files/new190718_12006.log
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user420
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/new190718_12006_fit_img.png
fits[0].softwareCRYSON
fits[0].chi_square_value4.123
fits[0].p_value0.000198
fits[0].fit_residual_plotnull
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDFQ3_fit1.fit
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/new190718_12006.log
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version4.6
i0_calibration_standardnull
descriptionnull
experiment_descriptionSANS data from solutions of the Asf1-H3:H4-Rtt109-Vps75 protein complex (1H Rtt109-H3:H4, 2H Asf1-Vps75) in 50 mM citrate, 150 mM NaCl, 5 mM BME, 42% D2O, pH 6.5 were collected on the KWS1 instrument the FRM2 (Munich, Germany) using a SANS 6Li-Scintillator 1 mm thickness + photomultiplier detector. Data were collected at two sample detector positions: 1) 1.5 m for 2 hrs using a neutron wavelength of 0.5 nm and; 2) 4 m for 2 hrs using a neutron wavelength of 0.5 nm. Both datasets were recorded on the same sample at 4.7 mg/mL, measured at 25°C.
tags[]
intensity_unitnull
experimental_mw146.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i00.2503
pddf_i0_errornull
guinier_i00.25
guinier_i0_error0.00092
pddf_rg3.66
pddf_rg_errornull
guinier_rg3.499
guinier_rg_error0.046
pddf_dmax11.0
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last12
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFQ3_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDFQ3
statussuccess
started_at2026-08-11T14:05:18.048424+00:00
finished_at2026-08-11T14:05:27.403046+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDFQ3/
files[0].path/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/summary.json
files[0].size12095
files[0].sha256bce670cdaba871932179fcdee95562026097870db81199e7afef25f5eeac875b
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDFQ3.dat
files[1].path/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.dat
files[1].size4561
files[1].sha2564874874d6a6e2355f632372577c071100ab2b7a69f94decb77244008c01eaef8
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDFQ3.out
files[2].path/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.out
files[2].size12893
files[2].sha256b180bf6b601da79530ffbf8358ab6ffbaee406566f1177bfe37e0497d422fb2a
files[2].rolepddf
files[3].statusdownloaded
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDFQ3.sascif
files[3].path/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.sascif
files[3].size1292487
files[3].sha256be52aa345fc87cf83641c3edd0cc1daa416df049ce4a72671f41fa91da2b4383
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDFQ3.zip
files[4].path/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.zip
files[4].size205079
files[4].sha25651cfd36ebd1e4c46e4bceb3210ada84085599a2b9d7c3a69983876bc3e6f687b
files[4].rolefull_entry_zip
files[4].zip_member_count4
files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDFQ3",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Histone chaperone exploits intrinsic disorder to switch acetylation specificity.",
    "publication": {
      "title": "Histone chaperone exploits intrinsic disorder to switch acetylation specificity.",
      "author_list": "Danilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-019-11410-7",
      "pmid": "31387991",
      "published_date": "2019 Aug 6"
    },
    "status": "released",
    "submitted_date": "2019-02-27",
    "released_date": "2019-07-10"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFQ3.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFQ3.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ3_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ3_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFQ3_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ3_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFQ3.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "SANS",
        "name": "6Li-Scintillator 1 mm thickness + photomultiplier",
        "resolution": 5.3
      },
      "name": "FRM2",
      "city": "Munich",
      "country": "Germany",
      "beamline_name": "KWS1",
      "beam_geometry": null,
      "type_of_source": "neutron source",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Vacuolar protein sorting-associated protein 75 (1-225 aa)",
          "short_name": "Vps75 1-225",
          "sequence": "MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\r\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\r\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\r\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLED",
          "organism": "Saccharomyces cerevisiae",
          "uniprot_code": "P53853",
          "uniprot_range_first": 1,
          "uniprot_range_last": 225,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSED\nDDGSLGEVDLPLSDEEPSSKKRKV",
          "mw": 26.554,
          "total_mw": 53.108,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Histone acetyltransferase RTT109",
          "short_name": "Rtt109",
          "sequence": "MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK\r\nVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL\r\nQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT\r\nCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA\r\nKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE\r\nDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA\r\nIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA\r\nITMLKPRKKAKALPKT",
          "organism": "Saccharomyces cerevisiae",
          "uniprot_code": "Q07794",
          "uniprot_range_first": 1,
          "uniprot_range_last": 436,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK\nVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL\nQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT\nCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA\nKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE\nDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA\nIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA\nITMLKPRKKAKALPKT",
          "mw": 50.095,
          "total_mw": 50.095,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Histone chaperone ASF1",
          "short_name": "Asf1",
          "sequence": "MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\r\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\r\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV",
          "organism": null,
          "uniprot_code": "P32447",
          "uniprot_range_first": 1,
          "uniprot_range_last": 169,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE\nDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE\nEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN",
          "mw": 19.125,
          "total_mw": 19.125,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Histone H3.2 (35-135 aa)",
          "short_name": "H3 (35-135)",
          "sequence": "VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAY \r\nLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA",
          "organism": "Xenopus laevis",
          "uniprot_code": "P84233",
          "uniprot_range_first": 35,
          "uniprot_range_last": 135,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE\nLLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI\nMPKDIQLARRIRGERA",
          "mw": 11.747,
          "total_mw": 11.747,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Histone H4",
          "short_name": "H4",
          "sequence": "MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\r\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG",
          "organism": "Xenopus laevis",
          "uniprot_code": "P62799",
          "uniprot_range_first": 1,
          "uniprot_range_last": 103,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG",
          "mw": 11.367,
          "total_mw": 11.367,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "50 mM citrate, 150 mM NaCl, 5 mM BME, 42% D2O",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 6.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Complex with 1H histone acetyltransferase Rtt109 and histones H3 and H4, 2H histone chaperones Asf1 and Vps75 (1H Rtt109-H3:H4, 2H Asf1-Vps75) acquired in 42% v/v D2O",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Germany",
            "full_name": "Leibniz University of Hannover",
            "webpage": "https://www.uni-hannover.de"
          }
        ],
        "contributor_name": "Nataliya",
        "contributor_surname": "Danilenko",
        "orcid": "https://orcid.org/0000-0003-4885-5051"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-03-05",
    "storage_temperature": 25.0,
    "cell_temperature": 25.0,
    "exposure_time": 7200.0,
    "number_of_frames": 1,
    "wavelength": 0.5,
    "sample_detector_distance": 4.0,
    "concentration_min": null,
    "concentration_max": 4.7,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.127,
    "s_max": 3.923,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFQ3_fit1_model1_img.png",
          "software": "HADDOCK",
          "pdb_link": [
            {
              "pdb_code": "6o22",
              "ext_code": "6O22",
              "ext_type": "pdb",
              "difference_with_model": "derived"
            }
          ],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFQ3_fit1_model1.pdb",
          "model_mw": 144.0,
          "bead_radius": null,
          "log": "https://www.sasbdb.org/media/log_files/new190718_12006.log",
          "symmetry": null,
          "comment": null,
          "user": 420
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/new190718_12006_fit_img.png",
      "software": "CRYSON",
      "chi_square_value": 4.123,
      "p_value": 0.000198,
      "fit_residual_plot": null,
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFQ3_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/new190718_12006.log",
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "4.6",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "SANS data from solutions of the Asf1-H3:H4-Rtt109-Vps75 protein complex (1H Rtt109-H3:H4, 2H Asf1-Vps75) in 50 mM citrate, 150 mM NaCl, 5 mM BME, 42% D2O, pH 6.5 were collected on the KWS1 instrument the FRM2 (Munich, Germany) using a SANS 6Li-Scintillator 1 mm thickness + photomultiplier detector. Data were collected at two sample detector positions: 1) 1.5 m for  2 hrs using a neutron wavelength of 0.5 nm and; 2) 4 m for 2 hrs using a neutron wavelength of 0.5 nm. Both datasets were recorded on the same sample at 4.7 mg/mL, measured at 25°C.",
  "tags": [],
  "intensity_unit": null,
  "experimental_mw": 146.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 0.2503,
  "pddf_i0_error": null,
  "guinier_i0": 0.25,
  "guinier_i0_error": 0.00092,
  "pddf_rg": 3.66,
  "pddf_rg_error": null,
  "guinier_rg": 3.499,
  "guinier_rg_error": 0.046,
  "pddf_dmax": 11.0,
  "pddf_dmax_error": null,
  "porod_volume": null,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 12,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFQ3_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
}
查看完整 manifest.json 原文
{
  "code": "SASDFQ3",
  "status": "success",
  "started_at": "2026-08-11T14:05:18.048424+00:00",
  "finished_at": "2026-08-11T14:05:27.403046+00:00",
  "source_last_modified": "2019-12-06T13:07:15.624532+01:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDFQ3/",
      "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/summary.json",
      "size": 12095,
      "sha256": "bce670cdaba871932179fcdee95562026097870db81199e7afef25f5eeac875b"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDFQ3.dat",
      "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.dat",
      "size": 4561,
      "sha256": "4874874d6a6e2355f632372577c071100ab2b7a69f94decb77244008c01eaef8",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDFQ3.out",
      "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.out",
      "size": 12893,
      "sha256": "b180bf6b601da79530ffbf8358ab6ffbaee406566f1177bfe37e0497d422fb2a",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFQ3.sascif",
      "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.sascif",
      "size": 1292487,
      "sha256": "be52aa345fc87cf83641c3edd0cc1daa416df049ce4a72671f41fa91da2b4383",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDFQ3.zip",
      "path": "/data/saxsdb/sasbdb/entries/q3/sasdfq3/source/SASDFQ3.zip",
      "size": 205079,
      "sha256": "51cfd36ebd1e4c46e4bceb3210ada84085599a2b9d7c3a69983876bc3e6f687b",
      "role": "full_entry_zip",
      "zip_member_count": 4,
      "zip_validation": "central_directory_readable"
    }
  ]
}