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SASDFQ6

DNA-binding protein HU-alpha, E34K

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2020-06-22T11:02:00.868249+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · DNA-binding protein HU-alpha, E34K

Concentration— – — Buffer / pH10 mM Bis-Tris, 100 mM NaCl / 7.5
Experimental temperature10.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.103 nmExposure3.0 s × 300

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
DNA-binding protein HU-alpha, E34K
View sequence
MNKTQLIDVIAEKAELSKTQAKAALESTLAAITKSLKEGDAVQLVGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK
proteinEscherichia coliP0ACF01–90dimerMolecule count 29.534 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1561[3]0.011428–0.323555 1/AHas uncertainty columnMissing 00sasbdb/entries/q6/sasdfq6/source/SASDFQ6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)6.67—Å
i0Guinier449.421.71arbitrary
i0P(r)442.6—arbitrary
mwExperimental20.0—kDa
porod_volumePorod36.0—ų
rgGuinier2.180.213Å
rgP(r)2.16—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name10 mM Bis-Tris, 100 mM NaClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2018-06-01Storage / measurement temperature— / 10.0
Exposure time3.0Frames300
Wavelength0.103Sample-to-detector distance1.5
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.114 – 3.236Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip：source record and ZIP contents (4 items)
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pddf：source record
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sascif：source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：156 field values
Field pathRaw value
codeSASDFQ6
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleNucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
project.publication.titleNucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
project.publication.author_listRemesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-020-16724-5
project.publication.pmid32518228
project.publication.published_date2020 Jun 9
project.statusreleased
project.submitted_date2019-04-26
project.released_date2020-04-21
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDFQ6.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameDNA-binding protein HU-alpha, E34K
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMNKTQLIDVIAEKAELSKTQAKAALESTLAAITKSLKEGDAVQLVGFGTFKVNHRAERTG RNPQTGKEIKIAAANVPAFVSGKALKDAVK
experiment.sample.molecule[0].organismEscherichia coli
experiment.sample.molecule[0].uniprot_codeP0ACF0
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last90
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNHRAERTG RNPQTGKEIKIAAANVPAFVSGKALKDAVK
experiment.sample.molecule[0].mw9.534
experiment.sample.molecule[0].total_mw19.068
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name10 mM Bis-Tris, 100 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDNA-binding protein HU-alpha, E34K
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameUCSF
experiment.contributor[0].affiliation[0].addressSan Francisco, CA, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of California, San Francisco (UCSF)
experiment.contributor[0].affiliation[0].webpagehttps://www.ucsf.edu/
experiment.contributor[0].contributor_nameSoumya
experiment.contributor[0].contributor_surnameG Remesh
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-06-01
experiment.storage_temperaturenull
experiment.cell_temperature10.0
experiment.exposure_time3.0
experiment.number_of_frames300
experiment.wavelength0.103
experiment.sample_detector_distance1.5
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.114
experiment.s_max3.236
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].models[0].softwareChimera
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fits[0].softwareFoXS
fits[0].chi_square_value4.034495
fits[0].p_value0.0
fits[0].fit_residual_plotSASDFQ6_fit1_fitresiduals_img.png
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fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw20.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i0442.6
pddf_i0_errornull
guinier_i0449.42
guinier_i0_error1.71
pddf_rg2.16
pddf_rg_errornull
guinier_rg2.18
guinier_rg_error0.213
pddf_dmax6.67
pddf_dmax_errornull
porod_volume36.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first23
guinier_point_last87
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFQ6_datloglog_img.png
symmetrynull
last_modified2020-06-22T11:02:00.868249+02:00
bragg_peak[]
manifest.json：36 field values
Field pathRaw value
codeSASDFQ6
statussuccess
started_at2026-08-11T16:52:47.381079+00:00
finished_at2026-08-11T16:52:56.278193+00:00
source_last_modified2020-06-22T11:02:00.868249+02:00
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View the complete summary.json source
{
  "code": "SASDFQ6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.",
    "publication": {
      "title": "Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.",
      "author_list": "Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-020-16724-5",
      "pmid": "32518228",
      "published_date": "2020 Jun 9"
    },
    "status": "released",
    "submitted_date": "2019-04-26",
    "released_date": "2020-04-21"
  },
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View the complete manifest.json source
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