SASDFR7

Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2022-08-22T09:53:00.976207+02:00
▼

1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant

Concentration— – 8.66 Buffer / pH20 mM HEPES / 7.0
Experimental temperature20.0 Facility / beamlineDépartement de Biochimie, Université de Montréal / Xenocs BioXolver L with MetalJet
Wavelength1.348 nmExposure60.0 s × 10

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Fructose-bisphosphate aldolase A
View sequence
>FBPALDOLASEPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLMPNMVTPGHACTQKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFISNHAY
proteinOryctolagus cuniculusP008832–364tetramerMolecule count 439.214 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1293[3]0.0106922151–0.399852081 1/AHas uncertainty columnMissing 00sasbdb/entries/r7/sasdfr7/source/SASDFR7.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.8—Å
i0Guinier0.13020.0002708arbitrary
i0P(r)0.13030.0002174arbitrary
mwExperimental157.3—kDa
mwGuinier I(0)160.0—kDa
mwPorod133.0—kDa
porod_volumePorod213.0—ų
rgGuinier3.6020.011Å
rgP(r)3.60.007Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

▼

3. Source Fits and Models Source Fits & Models

▶

4. Source File Index Source Files

▶

5. Experiment Description and Publication Experiment & Publication

▼

6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPESBuffer concentration—
pH7.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2019-06-14Storage / measurement temperature4.0 / 20.0
Exposure time60.0Frames10
Wavelength1.348Sample-to-detector distance600.0
SourceX-ray in houseDetectorPilatus3 R 300K
Facility / beamlineDépartement de Biochimie, Université de Montréal / Xenocs BioXolver L with MetalJet · Montréal, Canada
q range0.107 – 3.999Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
curvesasbdb/entries/r7/sasdfr7/source/SASDFR7.datdownloaded21313a5e13f2f58c3a974ceb740a57a53272497085f38e850472288c36b3cdc2c3a94DownloadView source fileSource site
full_entry_zipsasbdb/entries/r7/sasdfr7/source/SASDFR7.zipdownloaded525346172dc3d7c9e02ffc8cde9b832d9bb07248fc0cad128dec74d5aa155de1b07063DownloadView source fileSource site
pddfsasbdb/entries/r7/sasdfr7/source/SASDFR7.outdownloaded41713d8610632c38f7ea8aae6ca5a6892810e1643fd387a8042f426c5ce56336690b5DownloadView source fileSource site
sascifsasbdb/entries/r7/sasdfr7/source/SASDFR7.sascifdownloaded250318733049a0a07837cf26ff536de267f9a72ee74c34cc891b1a62e9336bded3ac8dcDownloadView source fileSource site
summarysasbdb/entries/r7/sasdfr7/source/summary.jsondownloaded878594bb4c837f0b954ea6f32236f78482155d9c44bed956edc0c6b6a0db4258c4e0DownloadView source fileSource site
curve:source record
{
  "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.dat",
  "role": "curve",
  "sha256": "a5e13f2f58c3a974ceb740a57a53272497085f38e850472288c36b3cdc2c3a94",
  "size": 21313,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/intensities_files/SASDFR7.dat"
}
full_entry_zip:source record and ZIP contents (6 items)
{
  "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.zip",
  "role": "full_entry_zip",
  "sha256": "172dc3d7c9e02ffc8cde9b832d9bb07248fc0cad128dec74d5aa155de1b07063",
  "size": 525346,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/zip_directories/SASDFR7.zip",
  "zip_member_count": 6,
  "zip_members": [
    {
      "crc32": "0ecf1619",
      "name": "SASDFR7/experimental_data/SASDFR7.dat",
      "size": 21313
    },
    {
      "crc32": "b37d0960",
      "name": "SASDFR7/pddf/SASDFR7.out",
      "size": 41713
    },
    {
      "crc32": "8f8bf2a1",
      "name": "SASDFR7/SASDFR7_fit1.fit",
      "size": 21443
    },
    {
      "crc32": "f8cbdb4d",
      "name": "SASDFR7/SASDFR7_fit1_model1.pdb",
      "size": 2004669
    },
    {
      "crc32": "888c0566",
      "name": "SASDFR7/SASDFR7_fit2.fir",
      "size": 17929
    },
    {
      "crc32": "a6ef59b7",
      "name": "SASDFR7/SASDFR7_fit2_model1.pdb",
      "size": 68501
    }
  ],
  "zip_validation": "central_directory_readable"
}
pddf:source record
{
  "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.out",
  "role": "pddf",
  "sha256": "d8610632c38f7ea8aae6ca5a6892810e1643fd387a8042f426c5ce56336690b5",
  "size": 41713,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/p_of_R_files/SASDFR7.out"
}
sascif:source record
{
  "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.sascif",
  "role": "sascif",
  "sha256": "33049a0a07837cf26ff536de267f9a72ee74c34cc891b1a62e9336bded3ac8dc",
  "size": 2503187,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFR7.sascif"
}
summary:source record
{
  "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/summary.json",
  "sha256": "94bb4c837f0b954ea6f32236f78482155d9c44bed956edc0c6b6a0db4258c4e0",
  "size": 8785,
  "status": "downloaded",
  "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDFR7/"
}

All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:177 field values
Field pathRaw value
codeSASDFR7
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleRabbit muscle fructose-1,6-bisphosphate aldolase K229M mutant
project.publicationnull
project.statusreleased
project.submitted_date2019-06-21
project.released_date2021-02-15
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDFR7.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDFR7.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR7_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR7_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFR7_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR7_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDFR7.sascif
experiment.instrument.detector.typepixel counting
experiment.instrument.detector.namePilatus3 R 300K
experiment.instrument.detector.resolution172.0
experiment.instrument.nameDépartement de Biochimie, Université de Montréal
experiment.instrument.cityMontréal
experiment.instrument.countryCanada
experiment.instrument.beamline_nameXenocs BioXolver L with MetalJet
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray in house
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameFructose-bisphosphate aldolase A
experiment.sample.molecule[0].short_nameAldolase
experiment.sample.molecule[0].sequence>FBP aldolase PHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFH ETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSA LAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLMPNMVTPGHACT QKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENL KAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFISNHAY
experiment.sample.molecule[0].organismOryctolagus cuniculus
experiment.sample.molecule[0].uniprot_codeP00883
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last364
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYR QLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTN GETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQ NGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHAC TQKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTF SYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFIS NHAY
experiment.sample.molecule[0].mw39.214
experiment.sample.molecule[0].total_mw156.856
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameRabbit muscle fructose-bisphosphate aldolase A, K229M mutant
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressMontréal, Canada
experiment.contributor[0].affiliation[0].full_nameDépartement de biochimie, Université de Montréal
experiment.contributor[0].affiliation[0].webpagehttp://www.umontreal.ca/
experiment.contributor[0].contributor_nameNormand
experiment.contributor[0].contributor_surnameCyr
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-9024-525X
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-06-14
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time60.0
experiment.number_of_frames10
experiment.wavelength1.348
experiment.sample_detector_distance600.0
experiment.concentration_minnull
experiment.concentration_max8.66
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.107
experiment.s_max3.999
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDFR7_fit1_model1_img.png
fits[0].models[0].softwarePDB (Protein Data Bank)
fits[0].models[0].pdb_link[0].pdb_code5f4x
fits[0].models[0].pdb_link[0].ext_code5F4X
fits[0].models[0].pdb_link[0].ext_typepdb
fits[0].models[0].pdb_link[0].difference_with_modelidentical
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
fits[0].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDFR7_fit1_model1.pdb
fits[0].models[0].model_mw157.0
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetryP222
fits[0].models[0].commentnull
fits[0].models[0].user462
fits[0].fit_unit1/A
fits[0].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFR7_fit1_fixed_fit_img.png
fits[0].softwareCRYSOL
fits[0].chi_square_value1.659
fits[0].p_value2.1e-05
fits[0].fit_residual_plotSASDFR7_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDFR7_fit1.fit
fits[0].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/5f4x01.log
fits[0].software_versionnull
fits[0].description
fits[1].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDFR7_fit2_model1_img.png
fits[1].models[0].softwareDAMMIN
fits[1].models[0].pdb_link[]
fits[1].models[0].model_titlenull
fits[1].models[0].type_of_modeldummy
fits[1].models[0].software_versionnull
fits[1].models[0].model_datahttps://www.sasbdb.org/media/pdb_file/SASDFR7_fit2_model1.pdb
fits[1].models[0].model_mw108.725
fits[1].models[0].bead_radius3.5
fits[1].models[0].loghttps://www.sasbdb.org/media/log_files/dammin.log
fits[1].models[0].symmetryP1
fits[1].models[0].commentnull
fits[1].models[0].user462
fits[1].fit_unit1/A
fits[1].fit_plothttps://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFR7_fit2_fixed_fit_img.png
fits[1].softwareDAMMIN
fits[1].chi_square_value1.178
fits[1].p_value0.008512
fits[1].fit_residual_plotSASDFR7_fit2_fitresiduals_img.png
fits[1].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDFR7_fit2.fir
fits[1].fit_loghttps://www.sasbdb.org/media/fitting_files/log_files/dammin_rLIHezm.log
fits[1].software_versionnull
fits[1].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSAXS data from solutions of rabbit muscle fructose-bisphosphate aldolase A (K229M) in 20 mM HEPES, pH 7 were collected using Xenocs BioXolver L instrument with MetalJet (Département de Biochimie, Université de Montréal, Canada) with a Pilatus3 R 300K detector at a wavelength of λ = 1.348 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.66 mg/ml was measured at 20°C. 10 successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw157.3
experimental_mw_errornull
guinier_i0_mw160.0
guinier_i0_mw_errornull
porod_mw133.0
porod_mw_errornull
pddf_i00.1303
pddf_i0_error0.0002174
guinier_i00.1302
guinier_i0_error0.0002708
pddf_rg3.6
pddf_rg_error0.007
guinier_rg3.602
guinier_rg_error0.011
pddf_dmax11.8
pddf_dmax_errornull
porod_volume213.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first1
guinier_point_last20
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFR7_datloglog_img.png
symmetrynull
last_modified2022-08-22T09:53:00.976207+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDFR7
statussuccess
started_at2026-08-11T15:37:04.965364+00:00
finished_at2026-08-11T15:37:21.699315+00:00
source_last_modified2022-08-22T09:53:00.976207+02:00
files[0].statusdownloaded
files[0].urlhttps://www.sasbdb.org/rest-api/entry/summary/SASDFR7/
files[0].path/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/summary.json
files[0].size8785
files[0].sha25694bb4c837f0b954ea6f32236f78482155d9c44bed956edc0c6b6a0db4258c4e0
files[1].statusdownloaded
files[1].urlhttps://www.sasbdb.org/media/intensities_files/SASDFR7.dat
files[1].path/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.dat
files[1].size21313
files[1].sha256a5e13f2f58c3a974ceb740a57a53272497085f38e850472288c36b3cdc2c3a94
files[1].rolecurve
files[2].statusdownloaded
files[2].urlhttps://www.sasbdb.org/media/p_of_R_files/SASDFR7.out
files[2].path/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.out
files[2].size41713
files[2].sha256d8610632c38f7ea8aae6ca5a6892810e1643fd387a8042f426c5ce56336690b5
files[2].rolepddf
files[3].statusdownloaded
files[3].urlhttps://www.sasbdb.org/media/sascif/sascif_files/SASDFR7.sascif
files[3].path/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.sascif
files[3].size2503187
files[3].sha25633049a0a07837cf26ff536de267f9a72ee74c34cc891b1a62e9336bded3ac8dc
files[3].rolesascif
files[4].statusdownloaded
files[4].urlhttps://www.sasbdb.org/media/zip_directories/SASDFR7.zip
files[4].path/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.zip
files[4].size525346
files[4].sha256172dc3d7c9e02ffc8cde9b832d9bb07248fc0cad128dec74d5aa155de1b07063
files[4].rolefull_entry_zip
files[4].zip_member_count6
files[4].zip_validationcentral_directory_readable
View the complete summary.json source
{
  "code": "SASDFR7",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Rabbit muscle fructose-1,6-bisphosphate aldolase K229M mutant",
    "publication": null,
    "status": "released",
    "submitted_date": "2019-06-21",
    "released_date": "2021-02-15"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFR7.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFR7.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR7_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR7_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFR7_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR7_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFR7.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "pixel counting",
        "name": "Pilatus3 R 300K",
        "resolution": 172.0
      },
      "name": "Département de Biochimie, Université de Montréal",
      "city": "Montréal",
      "country": "Canada",
      "beamline_name": "Xenocs BioXolver L with MetalJet",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Fructose-bisphosphate aldolase A",
          "short_name": "Aldolase",
          "sequence": ">FBP aldolase\r\nPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFH\r\nETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSA\r\nLAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLMPNMVTPGHACT\r\nQKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENL\r\nKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFISNHAY",
          "organism": "Oryctolagus cuniculus",
          "uniprot_code": "P00883",
          "uniprot_range_first": 2,
          "uniprot_range_last": 364,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MPHSHPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYR\nQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTN\nGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQ\nNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHAC\nTQKYSHEEIAMATVTALRRTVPPAVTGVTFLSGGQSEEEASINLNAINKCPLLKPWALTF\nSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFIS\nNHAY",
          "mw": 39.214,
          "total_mw": 156.856,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM HEPES",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Rabbit muscle fructose-bisphosphate aldolase A, K229M mutant",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Montréal, Canada",
            "full_name": "Département de biochimie, Université de Montréal",
            "webpage": "http://www.umontreal.ca/"
          }
        ],
        "contributor_name": "Normand",
        "contributor_surname": "Cyr",
        "orcid": "https://orcid.org/0000-0002-9024-525X"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2019-06-14",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 60.0,
    "number_of_frames": 10,
    "wavelength": 1.348,
    "sample_detector_distance": 600.0,
    "concentration_min": null,
    "concentration_max": 8.66,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.107,
    "s_max": 3.999,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFR7_fit1_model1_img.png",
          "software": "PDB (Protein Data Bank)",
          "pdb_link": [
            {
              "pdb_code": "5f4x",
              "ext_code": "5F4X",
              "ext_type": "pdb",
              "difference_with_model": "identical"
            }
          ],
          "model_title": null,
          "type_of_model": "atomic",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFR7_fit1_model1.pdb",
          "model_mw": 157.0,
          "bead_radius": null,
          "log": null,
          "symmetry": "P222",
          "comment": null,
          "user": 462
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFR7_fit1_fixed_fit_img.png",
      "software": "CRYSOL",
      "chi_square_value": 1.659,
      "p_value": 2.1e-05,
      "fit_residual_plot": "SASDFR7_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFR7_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/5f4x01.log",
      "software_version": null,
      "description": ""
    },
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDFR7_fit2_model1_img.png",
          "software": "DAMMIN",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "dummy",
          "software_version": null,
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDFR7_fit2_model1.pdb",
          "model_mw": 108.725,
          "bead_radius": 3.5,
          "log": "https://www.sasbdb.org/media/log_files/dammin.log",
          "symmetry": "P1",
          "comment": null,
          "user": 462
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFR7_fit2_fixed_fit_img.png",
      "software": "DAMMIN",
      "chi_square_value": 1.178,
      "p_value": 0.008512,
      "fit_residual_plot": "SASDFR7_fit2_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFR7_fit2.fir",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/dammin_rLIHezm.log",
      "software_version": null,
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "SAXS data from solutions of rabbit muscle fructose-bisphosphate aldolase A (K229M) in 20 mM HEPES, pH 7 were collected using Xenocs BioXolver L instrument with MetalJet (Département de Biochimie, Université de Montréal, Canada) with a Pilatus3 R 300K detector at a wavelength of λ = 1.348 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.66 mg/ml was measured at 20°C. 10 successive 60 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 157.3,
  "experimental_mw_error": null,
  "guinier_i0_mw": 160.0,
  "guinier_i0_mw_error": null,
  "porod_mw": 133.0,
  "porod_mw_error": null,
  "pddf_i0": 0.1303,
  "pddf_i0_error": 0.0002174,
  "guinier_i0": 0.1302,
  "guinier_i0_error": 0.0002708,
  "pddf_rg": 3.6,
  "pddf_rg_error": 0.007,
  "guinier_rg": 3.602,
  "guinier_rg_error": 0.011,
  "pddf_dmax": 11.8,
  "pddf_dmax_error": null,
  "porod_volume": 213.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 20,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFR7_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2022-08-22T09:53:00.976207+02:00",
  "bragg_peak": []
}
View the complete manifest.json source
{
  "code": "SASDFR7",
  "status": "success",
  "started_at": "2026-08-11T15:37:04.965364+00:00",
  "finished_at": "2026-08-11T15:37:21.699315+00:00",
  "source_last_modified": "2022-08-22T09:53:00.976207+02:00",
  "files": [
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/rest-api/entry/summary/SASDFR7/",
      "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/summary.json",
      "size": 8785,
      "sha256": "94bb4c837f0b954ea6f32236f78482155d9c44bed956edc0c6b6a0db4258c4e0"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/intensities_files/SASDFR7.dat",
      "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.dat",
      "size": 21313,
      "sha256": "a5e13f2f58c3a974ceb740a57a53272497085f38e850472288c36b3cdc2c3a94",
      "role": "curve"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/p_of_R_files/SASDFR7.out",
      "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.out",
      "size": 41713,
      "sha256": "d8610632c38f7ea8aae6ca5a6892810e1643fd387a8042f426c5ce56336690b5",
      "role": "pddf"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFR7.sascif",
      "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.sascif",
      "size": 2503187,
      "sha256": "33049a0a07837cf26ff536de267f9a72ee74c34cc891b1a62e9336bded3ac8dc",
      "role": "sascif"
    },
    {
      "status": "downloaded",
      "url": "https://www.sasbdb.org/media/zip_directories/SASDFR7.zip",
      "path": "/data/saxsdb/sasbdb/entries/r7/sasdfr7/source/SASDFR7.zip",
      "size": 525346,
      "sha256": "172dc3d7c9e02ffc8cde9b832d9bb07248fc0cad128dec74d5aa155de1b07063",
      "role": "full_entry_zip",
      "zip_member_count": 6,
      "zip_validation": "central_directory_readable"
    }
  ]
}