SASDFR9

Poly-uridine rU30 in 5 mM MgCl2

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Extrapolated to infinite dilution 最后更新:2019-12-11T18:17:14.164307+01:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Poly-uridine rU30 in 5 mM MgCl2

浓度0.4 – 1.2 缓冲液 / pH1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA / 7.0
实验温度25.0 设备 / 束线Cornell High Energy Synchrotron Source (CHESS) / G1
波长0.11 nm曝光10.0 s × 30

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Poly-uridine
查看序列
UUUUUUUUUUUUUUUUUUUUUUUUUUUUUU
RNA———–—monomer分子数 19.203 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1261[3]0.01–0.27 1/A含误差列缺失 01sasbdb/entries/r9/sasdfr9/source/SASDFR9.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)10.0—Å
i0Guinier30.40.31—
i0P(r)30.6——
mwExperimental13.0—kDa
porod_volumePorod15.0—ų
rgGuinier2.450.03Å
rgP(r)2.59—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

该条目没有来源拟合记录。

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA缓冲液浓度—
pH7.0添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2015-10-24储存 / 测量温度4.0 / 25.0
曝光时间10.0帧数30
波长0.11样品-探测器距离2.04
光源X-ray synchrotron探测器Finger Lakes CCD
机构 / 束线Cornell High Energy Synchrotron Source (CHESS) / G1 · Ithaca, NY, USA
q 范围0.1 – 2.7样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(2 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:134 个字段值
字段路径原始值
codeSASDFR9
statusPublished
type_of_curveExtrapolated to infinite dilution
angular_unit1/A
project.titleVisualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics.
project.publication.titleVisualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics.
project.publication.author_listPlumridge A, Andresen K, Pollack L
project.publication.journalJ Am Chem Soc
project.publication.doi10.1021/jacs.9b04461
project.publication.pmid31804813
project.publication.published_date2019 Dec 5
project.statusreleased
project.submitted_date2019-07-09
project.released_date2019-12-11
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experiment.instrument.detector.nameFinger Lakes CCD
experiment.instrument.detector.resolution0.0
experiment.instrument.nameCornell High Energy Synchrotron Source (CHESS)
experiment.instrument.cityIthaca, NY
experiment.instrument.countryUSA
experiment.instrument.beamline_nameG1
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePoly-uridine
experiment.sample.molecule[0].short_namerU30
experiment.sample.molecule[0].sequenceUUUUUUUUUUUUUUUUUUUUUUUUUUUUUU
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeRNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw9.203
experiment.sample.molecule[0].total_mw9.203
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePoly-uridine rU30 in 5 mM MgCl2
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressIthaca, NY, United States
experiment.contributor[0].affiliation[0].full_nameCornell University
experiment.contributor[0].affiliation[0].webpagehttps://www.cornell.edu/
experiment.contributor[0].contributor_nameAlex
experiment.contributor[0].contributor_surnamePlumridge
experiment.contributor[0].orcidnull
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2015-10-24
experiment.storage_temperature4.0
experiment.cell_temperature25.0
experiment.exposure_time10.0
experiment.number_of_frames30
experiment.wavelength0.11
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experiment.concentration_min0.4
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experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.1
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experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of rU30 in 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA, pH 7 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS) storage ring (Ithaca, NY, USA) using a Finger Lakes CCD detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.11 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 1.2 mg/ml were measured at 25°C. 30 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
tags[]
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guinier_i0_mwnull
guinier_i0_mw_errornull
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pddf_i0_errornull
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pddf_dmax_errornull
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porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first5
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pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFR9_datloglog_img.png
symmetrynull
last_modified2019-12-11T18:17:14.164307+01:00
bragg_peak[]
manifest.json:36 个字段值
字段路径原始值
codeSASDFR9
statussuccess
started_at2026-08-11T16:20:34.395479+00:00
finished_at2026-08-11T16:20:41.148597+00:00
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查看完整 summary.json 原文
{
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  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/A",
  "project": {
    "title": "Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics.",
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      "author_list": "Plumridge A, Andresen K, Pollack L",
      "journal": "J Am Chem Soc",
      "doi": "10.1021/jacs.9b04461",
      "pmid": "31804813",
      "published_date": "2019 Dec 5"
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}
查看完整 manifest.json 原文
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  "status": "success",
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