Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D

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  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
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    "title": "A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family.",
    "publication": {
      "title": "A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family.",
      "author_list": "Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC",
      "journal": "J Mol Biol",
      "doi": "10.1016/j.jmb.2019.05.002",
      "pmid": "31078555",
      "published_date": "2019 May 9"
    },
    "status": "released",
    "submitted_date": "2019-03-06",
    "released_date": "2019-05-16"
  },
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  "experiment": {
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      "detector": {
        "type": "Dectris",
        "name": "Pilatus 1M",
        "resolution": 172.0
      },
      "name": "Australian  Synchrotron",
      "city": "Melbourne",
      "country": "Australia",
      "beamline_name": "SAXS/WAXS",
      "beam_geometry": "Point",
      "type_of_source": "X-ray synchrotron",
      "point_source": true,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
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      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Transcription intermediary factor 1-beta",
          "short_name": "TRIM28",
          "sequence": "GPGEAQELLEHCGVCRERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPVCKQQCYSKDIVENYFMRDSGSKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKTRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNS",
          "organism": "Mus musculus",
          "uniprot_code": "Q62318",
          "uniprot_range_first": 58,
          "uniprot_range_last": 418,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MAASAAATAAASAATAASAASGSPGSGEGSAGGEKRPAASSAAAASAAASSPAGGGGEAQ\nELLEHCGVCRERLRPERDPRLLPCLHSACSACLGPATPAAANNSGDGGSAGDGAMVDCPV\nCKQQCYSKDIVENYFMRDSGSKASSDSQDANQCCTSCEDNAPATSYCVECSEPLCETCVE\nAHQRVKYTKDHTVRSTGPAKTRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKD\nHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKNTKEVRSSIRQVSDVQKRVQVDVKMAI\nLQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNT\nALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTG\nPGPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSDDPYSSAEPHVSGMKRSRSGEGEVSG\nLLRKVPRVSLERLDLDLTSDSQPPVFKVFPGSTTEDYNLIVIERGAAAAAAGQAGTVPPG\nAPGAPPLPGMAIVKEEETEAAIGAPPAAPEGPETKPVLMPLTEGPGAEGPRLASPSGSTS\nSGLEVVAPEVTSAPVSGPGILDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVP\nGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPL\nHQLATDSTFSMEQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKA\nDVQSIIGLQRFFETRMNDAFGDTKFSAVLVEPPPLNLPSAGLSSQELSGPGDGP",
          "mw": 40.928,
          "total_mw": 81.856,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
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        "name": "10 mM Tris 300 mM NaCl 0.1 mM TCEP",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
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      "purity_method": null,
      "name": "Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Auckland, New Zealand",
            "full_name": "The University of Auckland",
            "webpage": "https://www.auckland.ac.nz"
          }
        ],
        "contributor_name": "Yunyuan",
        "contributor_surname": "Sun",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-08-10",
    "storage_temperature": null,
    "cell_temperature": 16.0,
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    "wavelength": 0.113,
    "sample_detector_distance": 3.3,
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          "symmetry": "p2",
          "comment": null,
          "user": 423
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      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/RBCC_p2_b_fit_img.png",
      "software": "GASBOR",
      "chi_square_value": 0.33,
      "p_value": 0.012949,
      "fit_residual_plot": "RBCC_p2_b_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFS3_fit1.fir",
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  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of TRIM28 RBCC (R184D mutant) in 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 3.3 m and at a wavelength of λ = 0.113 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 16 °C using the following parameters: Column: GE-Healthcare S200 5/150 analytical SEC column; Flow rate: 0.5 mL/min; Sample injection concentration: 4.9 mg/mL; Injection volume: 50 μL. Total X-ray acquisition time = 690 s. The data were collected through the SEC peak of the protein as a series of 12 x 1 second exposures. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank was subtracted.",
  "tags": [],
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  "guinier_rg_error": 0.123,
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  "estimated_volume_error": null,
  "guinier_point_first": 4,
  "guinier_point_last": 45,
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
  "bragg_peak": []
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