HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:4DZL))

{
  "code": "SASDFS4",
  "status": "Published",
  "type_of_curve": "Extrapolated to infinite dilution",
  "angular_unit": "1/A",
  "project": {
    "title": "Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins.",
    "publication": {
      "title": "Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins.",
      "author_list": "Miyamoto T, Hayashi Y, Yoshida K, Watanabe H, Uchihashi T, Yonezawa K, Shimizu N, Kamikubo H, Hirota S",
      "journal": "ACS Synth Biol",
      "doi": "10.1021/acssynbio.9b00019",
      "pmid": "30966743",
      "published_date": "2019 Apr 16"
    },
    "status": "released",
    "submitted_date": "2019-03-28",
    "released_date": "2019-04-24"
  },
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  "experiment": {
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      "detector": {
        "type": "Pilatus",
        "name": "Pilatus 200K",
        "resolution": 172.0
      },
      "name": "Nara Institute of Science and Technology",
      "city": "Ikoma",
      "country": "Japan",
      "beamline_name": "Rigaku Nano-Viewer",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "HP0242 from Helicobacter pylori,  N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain",
          "short_name": "Fusion protein2",
          "sequence": "MGSSHHHHHHSSGLVPRGSHMRDYSELEIFEGNPLDKWNDIIFHASKKASKKELERLLELLALCETFIEKEDLEEKFESFAKALRIDEELQQKIESRKTDIVIQSMANILSALFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYGSGGEIAAIKQEIAAIKKEIAAIKWEIAAIKQGYG",
          "organism": "Helicobacter pylori,  Rattus norvegicus, de novo design",
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "hexamer",
          "molecular_type": "protein",
          "uniprot_sequence": null,
          "mw": 29.855,
          "total_mw": 179.13,
          "number_molecules": 6,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
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        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "HP0242 from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, de novo designed coiled-coil trimer domain (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:4DZL))",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Japan",
            "full_name": "Nara Institute of Science and Technology",
            "webpage": "http://www.naist.jp/en/"
          }
        ],
        "contributor_name": "Hironari",
        "contributor_surname": "Kamikubo",
        "orcid": "https://orcid.org/0000-0002-0878-6488"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2017-03-29",
    "storage_temperature": 4.0,
    "cell_temperature": 20.0,
    "exposure_time": 60.0,
    "number_of_frames": 25,
    "wavelength": 0.15418,
    "sample_detector_distance": 0.75,
    "concentration_min": 1.9,
    "concentration_max": 6.8,
    "sample_volume": null,
    "flow_rate": null,
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    "s_max": 3.003,
    "total_exposure_time": null,
    "seccolumn": null
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      "chi_square_value": 0.1,
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      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDFS4_fit1.fit",
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      "software_version": "5",
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  "experiment_description": "SAXS data from solutions of the fusion protein2 in 20 mM Tris-HCl, 150 mM KCl, pH 8.0 were collected using a Nano-Viewer (Rigaku, Japan) at NAIST (Nara, Japan) equipped with a Pilatus 200K detector at a sample-detector distance of 0.75 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Twenty-five successive 60 second frames were collected at a sample temperature of 20°C. Solute concentrations ranging between 1.9 and 6.8 mg/ml were measured. The data were normalized to the intensity of the direct beam and radially averaged. The scattering from the solvent-blank was subtracted. The scattering profile in this entry represents an extrapolation to infinite dilution.",
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  "symmetry": null,
  "last_modified": "2019-12-06T13:07:15.624532+01:00",
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