SASDFT6

DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2020-06-22T11:02:12.995419+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)

Concentration— – — Buffer / pH20mM HEPES, 100mM NaCl / 7.5
Experimental temperature10.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.103 nmExposure3.0 s × 300

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
80bp_DNA Forward
View sequence
AATGAGGTAACAACGAAAGCAGATGATAGCTGCTTATCAATTTGTTGCAAACAAGTAGCCGCGCCCAATGAGGTAACAAT
DNAEscherichia coli——–—monomerMolecule count 124.849 kDa
80bp_DNA Reverse
View sequence
TTACTCCATTGTTGCTTTCGTCTACTATCGACGAATAGTTAAACAACGTTTGTTCATCGGCGCGGGTTACTCCATTGTTA
DNAEscherichia coli——–—monomerMolecule count 124.612 kDa
DNA-binding protein HU-alpha, E34K
View sequence
MNKTQLIDVIAEKAELSKTQAKAALESTLAAITKSLKEGDAVQLVGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK
proteinEscherichia coli—1–90otherMolecule count 289.534 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1561[3]0.011428–0.323555 1/AHas uncertainty columnMissing 00sasbdb/entries/t6/sasdft6/source/SASDFT6.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)27.0—Å
i0Guinier63.820.64arbitrary
i0P(r)65.42—arbitrary
mwExperimental360.0—kDa
porod_volumePorod474.0—ų
rgGuinier7.1880.57Å
rgP(r)7.82—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM HEPES, 100mM NaClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-07-08Storage / measurement temperature— / 10.0
Exposure time3.0Frames300
Wavelength0.103Sample-to-detector distance1.5
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.114 – 3.236Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (5 items)
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pddf:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:203 field values
Field pathRaw value
codeSASDFT6
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleNucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
project.publication.titleNucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
project.publication.author_listRemesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-020-16724-5
project.publication.pmid32518228
project.publication.published_date2020 Jun 9
project.statusreleased
project.submitted_date2019-04-26
project.released_date2020-04-21
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experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_name80bp_DNA Forward
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceAATGAGGTAACAACGAAAGCAGATGATAGCTGCTTATCAATTTGTTGCAAACAAGTAGCCGCGCCCAATGAGGTAACAAT
experiment.sample.molecule[0].organismEscherichia coli
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequencenull
experiment.sample.molecule[0].mw24.849
experiment.sample.molecule[0].total_mw24.849
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_name80bp_DNA Reverse
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequenceTTACTCCATTGTTGCTTTCGTCTACTATCGACGAATAGTTAAACAACGTTTGTTCATCGGCGCGGGTTACTCCATTGTTA
experiment.sample.molecule[1].organismEscherichia coli
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeDNA
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw24.612
experiment.sample.molecule[1].total_mw24.612
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.molecule[2].long_nameDNA-binding protein HU-alpha, E34K
experiment.sample.molecule[2].short_namenull
experiment.sample.molecule[2].sequenceMNKTQLIDVI AEKAELSKTQ AKAALESTLA AITKSLKEGD AVQLVGFGTF KVNHRAERTG RNPQTGKEIK IAAANVPAFV SGKALKDAVK
experiment.sample.molecule[2].organismEscherichia coli
experiment.sample.molecule[2].uniprot_codenull
experiment.sample.molecule[2].uniprot_range_first1
experiment.sample.molecule[2].uniprot_range_last90
experiment.sample.molecule[2].oligomerizationother
experiment.sample.molecule[2].molecular_typeprotein
experiment.sample.molecule[2].uniprot_sequencenull
experiment.sample.molecule[2].mw9.534
experiment.sample.molecule[2].total_mw266.952
experiment.sample.molecule[2].number_molecules28
experiment.sample.molecule[2].complex_stateFalse
experiment.sample.molecule[2].deuterationnull
experiment.sample.molecule[2].molecule_sourcebiological
experiment.sample.molecule[2].molecule_descriptionnull
experiment.sample.buffer.name20mM HEPES, 100mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameDNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (ratio DNA:Protein 1:5)
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameUCSF
experiment.contributor[0].affiliation[0].addressSan Francisco, CA, USA
experiment.contributor[0].affiliation[0].full_nameUniversity of California, San Francisco (UCSF)
experiment.contributor[0].affiliation[0].webpagehttps://www.ucsf.edu/
experiment.contributor[0].contributor_nameSoumya
experiment.contributor[0].contributor_surnameG Remesh
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2016-07-08
experiment.storage_temperaturenull
experiment.cell_temperature10.0
experiment.exposure_time3.0
experiment.number_of_frames300
experiment.wavelength0.103
experiment.sample_detector_distance1.5
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.114
experiment.s_max3.236
experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwareFoXS
fits[0].chi_square_value3.8362
fits[0].p_value0.0
fits[0].fit_residual_plotSASDFT6_fit1_fitresiduals_img.png
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fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw360.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i065.42
pddf_i0_errornull
guinier_i063.82
guinier_i0_error0.64
pddf_rg7.82
pddf_rg_errornull
guinier_rg7.188
guinier_rg_error0.57
pddf_dmax27.0
pddf_dmax_errornull
porod_volume474.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_firstnull
guinier_point_lastnull
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFT6_datloglog_img.png
symmetrynull
last_modified2020-06-22T11:02:12.995419+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDFT6
statussuccess
started_at2026-08-11T16:37:18.090765+00:00
finished_at2026-08-11T16:37:29.011574+00:00
source_last_modified2020-06-22T11:02:12.995419+02:00
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View the complete summary.json source
{
  "code": "SASDFT6",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.",
    "publication": {
      "title": "Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.",
      "author_list": "Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-020-16724-5",
      "pmid": "32518228",
      "published_date": "2020 Jun 9"
    },
    "status": "released",
    "submitted_date": "2019-04-26",
    "released_date": "2020-04-21"
  },
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          "organism": "Escherichia coli",
          "uniprot_code": null,
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View the complete manifest.json source
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