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SASDFV9

Adhesion G-protein coupled receptor G6 - zfGpr126 S2 (-ss) D134A/F135A ECR

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2019-12-17T18:35:00.450949+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Adhesion G-protein coupled receptor G6 - zfGpr126 S2 (-ss) D134A/F135A ECR

Concentration— – 2.5 Buffer / pH150 mM NaCl, 20 mM HEPES / 7.5
Experimental temperature22.0 Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID
Wavelength0.103 nmExposure0.8 s × 1185

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Adhesion G-protein coupled receptor G6 S2 D134A/F135A
View sequence
ASTSCNVVLTDSQGSFTSPCYPNDYPPSQSCNWTIQAPAGFIVQITFLDFELEEAQGCIYDRVVVKTGTSDAKFCGLTANGLTLNSTGNVMEVFFNSAASVQKKGFHISYKQVAVTLRNQKVTMPKSSKTILRVSNSISIPVLTAFTVCFEIARTAQKATETIFTLSDAAGTSILAFEKTSNGMELFIGASYCSVDNFLTSSDITATMKPLCLTWTKSSGLIGVYFEGHYFSSICSASQIYTLQSGGLLQIAGKGSSSVSVDDQNLDGFIYNFRLWDHAMLSSELSALTCDTVGNVVDWDHSYWTIPGSSTQTDSTLSCSTAITTLSPGTAGCASGLGCPEDIFYRSTLVVTDEQTPDRDATAIISQWLNQTFQNWMYRVYVDGISLQLITVLSRITTTRQIYLALLVYKNTTDVNLAEVEIESMLRSAPAIGNGLTLDSVTVNLMENCQADEFPVHYRWPESRPTVTQYVPCFPYKDRNASRTCMINRDNYTSFWALPDRGNCTNITSITVSQENAMDVAVQLADISNNGLSKEELTQVVTKVMELVNIAKINATLASTVVTIISNVMVSSEDAQKDASETALKAVDELVQKIEFDGPSLTISSKNLVVGVSALDTTNFNGSTLSAFIATNTTDPQIDFDSEAHNALAVVTLPPTLLQNLSLSQIEKVSRINFMFFGRTGLFQDHQNNGLTLNSYVVASSVGNFTIKNLQDPVRIEIAHLEYQKDPNPQCVFWDFNLQNYSGGWNSDGCKVGSDSNSNRTVCLCNHLTHFGILMDVSRAHHHHHHHH
proteinDanio rerioC6KFA339–839monomerMolecule count 186.25 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11185[3]0.00519627376–0.366601087 1/AHas uncertainty columnMissing 063sasbdb/entries/v9/sasdfv9/source/SASDFV9.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)14.8—Å
i0Guinier153.62110.4943arbitrary
i0P(r)153.9—arbitrary
mwExperimental95.0—kDa
mwPorod113.0—kDa
porod_volumePorod181.0—ų
rgGuinier4.280.023Å
rgP(r)4.32—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name150 mM NaCl, 20 mM HEPESBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2018-06-28Storage / measurement temperature4.0 / 22.0
Exposure time0.8Frames1185
Wavelength0.103Sample-to-detector distance1.0
SourceX-ray synchrotronDetectorPilatus3 X 1M
Facility / beamlineAdvanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID · Lemont, IL, USA
q range0.052 – 3.666Sample volume / flow rate200.0 / 0.7

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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curve：source record
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full_entry_zip：source record and ZIP contents (2 items)
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pddf：source record
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summary：source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json：134 field values
Field pathRaw value
codeSASDFV9
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructural basis for adhesion G protein-coupled receptor Gpr126 function
project.publication.titleStructural basis for adhesion G protein-coupled receptor Gpr126 function
project.publication.author_listLeon K, Cunningham R, Riback J, Feldman E, Li J, Sosnick T, Zhao M, Monk K, Araç D
project.publication.journalNature Communications
project.publication.doi10.1038/s41467-019-14040-1
project.publication.pmidnull
project.publication.published_date2020 Jan 10
project.statusreleased
project.submitted_date2019-07-18
project.released_date2019-12-17
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDFV9.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDFV9.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFV9_dat_img.png
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sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDFV9.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 1M
experiment.instrument.detector.resolution0.172
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBioCAT 18ID
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameAdhesion G-protein coupled receptor G6 S2 D134A/F135A
experiment.sample.molecule[0].short_nameGpr126 D134A/F135A
experiment.sample.molecule[0].sequenceASTSCNVVLTDSQGSFTSPCYPNDYPPSQSCNWTIQAPAGFIVQITFLDFELEEAQGCIYDRVVVKTGTSDAKFCGLTANGLTLNSTGNVMEVFFNSAASVQKKGFHISYKQVAVTLRNQKVTMPKSSKTILRVSNSISIPVLTAFTVCFEIARTAQKATETIFTLSDAAGTSILAFEKTSNGMELFIGASYCSVDNFLTSSDITATMKPLCLTWTKSSGLIGVYFEGHYFSSICSASQIYTLQSGGLLQIAGKGSSSVSVDDQNLDGFIYNFRLWDHAMLSSELSALTCDTVGNVVDWDHSYWTIPGSSTQTDSTLSCSTAITTLSPGTAGCASGLGCPEDIFYRSTLVVTDEQTPDRDATAIISQWLNQTFQNWMYRVYVDGISLQLITVLSRITTTRQIYLALLVYKNTTDVNLAEVEIESMLRSAPAIGNGLTLDSVTVNLMENCQADEFPVHYRWPESRPTVTQYVPCFPYKDRNASRTCMINRDNYTSFWALPDRGNCTNITSITVSQENAMDVAVQLADISNNGLSKEELTQVVTKVMELVNIAKINATLASTVVTIISNVMVSSEDAQKDASETALKAVDELVQKIEFDGPSLTISSKNLVVGVSALDTTNFNGSTLSAFIATNTTDPQIDFDSEAHNALAVVTLPPTLLQNLSLSQIEKVSRINFMFFGRTGLFQDHQNNGLTLNSYVVASSVGNFTIKNLQDPVRIEIAHLEYQKDPNPQCVFWDFNLQNYSGGWNSDGCKVGSDSNSNRTVCLCNHLTHFGILMDVSRAHHHHHHHH
experiment.sample.molecule[0].organismDanio rerio
experiment.sample.molecule[0].uniprot_codeC6KFA3
experiment.sample.molecule[0].uniprot_range_first39
experiment.sample.molecule[0].uniprot_range_last839
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMISFISGRWWRWKFQNTLAVFLLLICLSTSVAQSCQSSTSCNVVLTDSQGSFTSPCYPND YPPSQSCNWTIQAPAGFIVQITFLDFELEEAQGCIYDRVVVKTGTSDAKFCGLTANGLTL NSTGNVMEVFFNSDFSVQKKGFHISYKQVAVTLRNQKVTMPKSSKTILRVSNSISIPVLT AFTVCFEIARTAQKATETIFTLSDAAGTSILAFEKTSNGMELFIGASYCSVDNLLTSSDI TATMKPLCLTWTKSSGLIGVYFGGHYFSSICSASQIYTLQSGGLLQIAGKGSSSVSVDDQ NLDGFIYNFRLWDHAMLSSELSALTCDTVGNVVDWDHSYWTIPGSSTQTDSTLSCSTAIT TLSPGTAGCASGLGCPATLTVTITSIATTNIIPTNATTHEDIFYRSTLVVTDEQTPDRDA TAIISQWLNQTFQNWMYRVYVDGISLQLITVLSRITTTRQTYLALLVYKNTTDVNLAEVE IESMLRSAPAIGNGLTLDSVTVNLMENCQADEFPVHYRWPESRPTVTQYVPCFPYKDRNA SRTCMINRDNYTSFWALPDRGNCTNITSITVSQENAMDVAVQLADISNNGLSKEELTQVV TKVMELVNIAKINATLASTVVTIISNVMVSSEDAQKDASETALKAVDELVQKIEFDGPSL TISSKNLVVGVSALDTTNFNGSTLSAFIATNTTDPQIDFDSEAHNALAVVTLPPTLLQNL SLSQIEKVSRINFMFFGRTGLFQDHQNNGLTLNSYVVASSVGNFTIKNLQDPVRIEIAHL EYQKDPNPQCVFWDFNLQNYSGGCNSDGCKVGSDSNSNRTVCLCNHLTHFGILMDVSRAA ELIDEKNNRVLTFITYIGCGISAIFSAATLLTYIAFEKLRRDYPSKILMNLSTSLLFLNM VFLLDGWLASYEIKELCVTVAVFLHFFLLTSFTWMGLESIHMYIALVKVFNTYIRRYILK FCIVGWGVPAAIVGIVLAVSKDSYGKNYYGKGKDGQGTSEFCWILNPVVFYVTCVAYFSI IFLMNVAMFIVVMIQICGRNGKRSNRTLREDILRNLRSVVSLTFLLGMTWGFAFFAWGPV SLAFMYLFTIFNSLQGLFIFVFHCALKENVQKQWRRYLCCGKLRLADNSDWSKTATNNTK KVSSDNLGKSLSSSSFGSTTANWTSKAKATLNPFARHSNADSTLQ
experiment.sample.molecule[0].mw86.25
experiment.sample.molecule[0].total_mw86.25
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name150 mM NaCl, 20 mM HEPES
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameAdhesion G-protein coupled receptor G6 - zfGpr126 S2 (-ss) D134A/F135A ECR
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_nameUchicago
experiment.contributor[0].affiliation[0].addressEdward H. Levi Hall 5801 South Ellis Avenue Chicago, Illinois 60637 773.702.1234
experiment.contributor[0].affiliation[0].full_nameThe University of Chicago
experiment.contributor[0].affiliation[0].webpagehttp://www.uchicago.edu/
experiment.contributor[0].contributor_nameKatherine
experiment.contributor[0].contributor_surnameLeon
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-06-28
experiment.storage_temperature4.0
experiment.cell_temperature22.0
experiment.exposure_time0.8
experiment.number_of_frames1185
experiment.wavelength0.103
experiment.sample_detector_distance1.0
experiment.concentration_minnull
experiment.concentration_max2.5
experiment.sample_volume200.0
experiment.flow_rate0.7
experiment.s_min0.052
experiment.s_max3.666
experiment.total_exposure_timenull
experiment.seccolumn1
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw95.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw113.0
porod_mw_errornull
pddf_i0153.9
pddf_i0_errornull
guinier_i0153.6211
guinier_i0_error0.4943
pddf_rg4.32
pddf_rg_errornull
guinier_rg4.28
guinier_rg_error0.023
pddf_dmax14.8
pddf_dmax_errornull
porod_volume181.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first5
guinier_point_last77
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDFV9_datloglog_img.png
symmetrynull
last_modified2019-12-17T18:35:00.450949+01:00
bragg_peak[]
manifest.json：36 field values
Field pathRaw value
codeSASDFV9
statussuccess
started_at2026-08-11T16:22:42.037533+00:00
finished_at2026-08-11T16:22:51.248691+00:00
source_last_modified2019-12-17T18:35:00.450949+01:00
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View the complete summary.json source
{
  "code": "SASDFV9",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural basis for adhesion G protein-coupled receptor Gpr126 function",
    "publication": {
      "title": "Structural basis for adhesion G protein-coupled receptor Gpr126 function",
      "author_list": "Leon K, Cunningham R, Riback J, Feldman E, Li J, Sosnick T, Zhao M, Monk K, Araç D",
      "journal": "Nature Communications",
      "doi": "10.1038/s41467-019-14040-1",
      "pmid": null,
      "published_date": "2020 Jan 10"
    },
    "status": "released",
    "submitted_date": "2019-07-18",
    "released_date": "2019-12-17"
  },
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        "resolution": 0.172
      },
      "name": "Advanced Photon Source (APS), Argonne National Laboratory",
      "city": "Lemont, IL",
      "country": "USA",
      "beamline_name": "BioCAT 18ID",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
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      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Adhesion G-protein coupled receptor G6 S2 D134A/F135A",
          "short_name": "Gpr126 D134A/F135A",
          "sequence": "ASTSCNVVLTDSQGSFTSPCYPNDYPPSQSCNWTIQAPAGFIVQITFLDFELEEAQGCIYDRVVVKTGTSDAKFCGLTANGLTLNSTGNVMEVFFNSAASVQKKGFHISYKQVAVTLRNQKVTMPKSSKTILRVSNSISIPVLTAFTVCFEIARTAQKATETIFTLSDAAGTSILAFEKTSNGMELFIGASYCSVDNFLTSSDITATMKPLCLTWTKSSGLIGVYFEGHYFSSICSASQIYTLQSGGLLQIAGKGSSSVSVDDQNLDGFIYNFRLWDHAMLSSELSALTCDTVGNVVDWDHSYWTIPGSSTQTDSTLSCSTAITTLSPGTAGCASGLGCPEDIFYRSTLVVTDEQTPDRDATAIISQWLNQTFQNWMYRVYVDGISLQLITVLSRITTTRQIYLALLVYKNTTDVNLAEVEIESMLRSAPAIGNGLTLDSVTVNLMENCQADEFPVHYRWPESRPTVTQYVPCFPYKDRNASRTCMINRDNYTSFWALPDRGNCTNITSITVSQENAMDVAVQLADISNNGLSKEELTQVVTKVMELVNIAKINATLASTVVTIISNVMVSSEDAQKDASETALKAVDELVQKIEFDGPSLTISSKNLVVGVSALDTTNFNGSTLSAFIATNTTDPQIDFDSEAHNALAVVTLPPTLLQNLSLSQIEKVSRINFMFFGRTGLFQDHQNNGLTLNSYVVASSVGNFTIKNLQDPVRIEIAHLEYQKDPNPQCVFWDFNLQNYSGGWNSDGCKVGSDSNSNRTVCLCNHLTHFGILMDVSRAHHHHHHHH",
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          "uniprot_code": "C6KFA3",
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View the complete manifest.json source
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