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{
"code": "SASDFW6",
"status": "Published",
"type_of_curve": "Single concentration",
"angular_unit": "1/A",
"project": {
"title": "Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)",
"publication": null,
"status": "released",
"submitted_date": "2019-06-04",
"released_date": "2020-04-21"
},
"pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFW6.out",
"intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFW6.dat",
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"experiment": {
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},
"name": "Advanced Light Source (ALS)",
"city": "Berkeley, CA",
"country": "USA",
"beamline_name": "12.3.1 (SIBYLS)",
"beam_geometry": "",
"type_of_source": "X-ray synchrotron",
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},
"sample": {
"molecule": [
{
"long_name": "80bp_DNA Forward",
"short_name": null,
"sequence": "AATGAGGTAACAACGAAAGCAGATGATAGCTGCTTATCAATTTGTTGCAAACAAGTAGCCGCGCCCAATGAGGTAACAAT",
"organism": "Escherichia coli",
"uniprot_code": null,
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"oligomerization": "monomer",
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},
{
"long_name": "80bp_DNA Reverse",
"short_name": null,
"sequence": "TTACTCCATTGTTGCTTTCGTCTACTATCGACGAATAGTTAAACAACGTTTGTTCATCGGCGCGGGTTACTCCATTGTTA",
"organism": "Escherichia coli",
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},
{
"long_name": "DNA-binding protein HU-alpha, E38K/V42L double mutant",
"short_name": null,
"sequence": "MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKKGDALQLVGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK",
"organism": "Escherichia coli",
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"oligomerization": "tetramer",
"molecular_type": "protein",
"uniprot_sequence": "MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNHRAERTG\nRNPQTGKEIKIAAANVPAFVSGKALKDAVK",
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}
],
"buffer": {
"name": "50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF",
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"pka": null,
"ph": 7.5,
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},
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"name": "DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2)",
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"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
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"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": "UCSF",
"address": "San Francisco, CA, USA",
"full_name": "University of California, San Francisco (UCSF)",
"webpage": "https://www.ucsf.edu/"
}
],
"contributor_name": "Soumya",
"contributor_surname": "G Remesh",
"orcid": ""
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2015-04-23",
"storage_temperature": null,
"cell_temperature": 10.0,
"exposure_time": 0.1,
"number_of_frames": 32,
"wavelength": 0.1,
"sample_detector_distance": 1.5,
"concentration_min": null,
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"s_min": 0.1,
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},
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},
{
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"software": "FoXS",
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}
],
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"pddf_software": "ATSAS GNOM",
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"description": null,
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"tags": [],
"intensity_unit": "arbitrary",
"experimental_mw": 62.0,
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"porod_mw": null,
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"guinier_point_first": 1,
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"pddf_point_first": null,
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"intensities_log_log_plot": "SASDFW6_datloglog_img.png",
"symmetry": null,
"last_modified": "2020-06-22T12:20:01.086855+02:00",
"bragg_peak": []
}
