Apolipoprotein E2 (ApoE2) bound to 12 µg/mL heparin

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  "code": "SASDFW8",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Therapeutic approaches to ApoE",
    "publication": {
      "title": "Therapeutic approaches to ApoE",
      "author_list": "Lucas Kraft",
      "journal": "University of Sussex PhD thesis",
      "doi": null,
      "pmid": null,
      "published_date": "2019 April 25"
    },
    "status": "released",
    "submitted_date": "2019-10-25",
    "released_date": "2021-09-27"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDFW8.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDFW8.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFW8_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFW8_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFW8_pofr_img.png",
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  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDFW8.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": "1 x 5 mm",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Apolipoprotein E2",
          "short_name": "ApoE2",
          "sequence": "GPHMKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKCLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH",
          "organism": "Homo sapiens",
          "uniprot_code": "P02649",
          "uniprot_range_first": 19,
          "uniprot_range_last": 317,
          "oligomerization": "tetramer",
          "molecular_type": "protein",
          "uniprot_sequence": "MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQT\nLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQA\nRLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVY\nQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMG\nSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEK\nVQAAVGTSAAPVPSDNH",
          "mw": 34.606,
          "total_mw": 138.848,
          "number_molecules": 4,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        },
        {
          "long_name": "Heparin",
          "short_name": null,
          "sequence": "(C12H19NO20S3)n",
          "organism": null,
          "uniprot_code": null,
          "uniprot_range_first": null,
          "uniprot_range_last": null,
          "oligomerization": "monomer",
          "molecular_type": "other",
          "uniprot_sequence": null,
          "mw": 15.0,
          "total_mw": 15.0,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": null
        }
      ],
      "buffer": {
        "name": "20 mM HEPES, 300 mM NaCl, 1 mM TCEP",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Apolipoprotein E2 (ApoE2) bound to 12 µg/mL heparin",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Brighton, UK",
            "full_name": "University of Sussex",
            "webpage": "http://www.sussex.ac.uk/"
          }
        ],
        "contributor_name": "Lucas",
        "contributor_surname": "Kraft",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-05-03",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 1.0,
    "number_of_frames": 28,
    "wavelength": 0.1,
    "sample_detector_distance": 4.036,
    "concentration_min": null,
    "concentration_max": 4.0,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.03,
    "s_max": 3.7,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "2.8.4",
  "i0_calibration_standard": null,
  "description": "ApoE2 + 12 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.",
  "experiment_description": "Synchrotron SAXS data from solutions of ApoE2 bound to 12 µg/mL heparin in 20 mM HEPES, 300 mM NaCl, 1 mM TCEP, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 20°C. 28 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 194.0,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": 0.5055,
  "pddf_i0_error": null,
  "guinier_i0": 0.509,
  "guinier_i0_error": 0.0046,
  "pddf_rg": 6.46,
  "pddf_rg_error": null,
  "guinier_rg": 6.38,
  "guinier_rg_error": 0.082,
  "pddf_dmax": 21.7,
  "pddf_dmax_error": null,
  "porod_volume": 531.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 35,
  "guinier_point_last": 75,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDFW8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2024-10-07T16:04:49.386966+02:00",
  "bragg_peak": []
}

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