SASDG28

Apolipoprotein E4 (methylated) bound to 37 µg/mL heparin

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2024-10-07T16:02:56.037606+02:00

ApoE4 (methylated) + 37 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Apolipoprotein E4 (methylated) bound to 37 µg/mL heparin

Concentration— – 4.0 Buffer / pH20 mM HEPES, 300 mM NaCl, 1 mM TCEP / 8.0
Experimental temperature20.0 Facility / beamlineDiamond Light Source / B21
Wavelength0.1 nmExposure1.0 s × 28

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Apolipoprotein E4
View sequence
GPHMKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH
proteinHomo sapiensP0264919–317tetramerMolecule count 434.712 kDa
Heparin
View sequence
(C12H19NO20S3)N
other———–—monomerMolecule count 115.0 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11650[3]0.003–0.37 1/AHas uncertainty columnMissing 010sasbdb/entries/28/sasdg28/source/SASDG28.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)22.2—Å
i0Guinier0.50.0032arbitrary
i0P(r)0.5008—arbitrary
mwExperimental196.0—kDa
rgGuinier6.3920.061Å
rgP(r)6.58—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 300 mM NaCl, 1 mM TCEPBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2018-05-03Storage / measurement temperature20.0 / 20.0
Exposure time1.0Frames28
Wavelength0.1Sample-to-detector distance4.036
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.03 – 3.7Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:151 field values
Field pathRaw value
codeSASDG28
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleTherapeutic approaches to ApoE
project.publication.titleTherapeutic approaches to ApoE
project.publication.author_listLucas Kraft
project.publication.journalUniversity of Sussex PhD thesis
project.publication.doinull
project.publication.pmidnull
project.publication.published_date2019 April 25
project.statusreleased
project.submitted_date2019-10-25
project.released_date2021-09-27
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experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometry1 x 5 mm
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameApolipoprotein E4
experiment.sample.molecule[0].short_nameApoE4
experiment.sample.molecule[0].sequenceGPHMKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP02649
experiment.sample.molecule[0].uniprot_range_first19
experiment.sample.molecule[0].uniprot_range_last317
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQT LSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQA RLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVY QAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMG SRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEK VQAAVGTSAAPVPSDNH
experiment.sample.molecule[0].mw34.712
experiment.sample.molecule[0].total_mw138.848
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameHeparin
experiment.sample.molecule[1].short_namenull
experiment.sample.molecule[1].sequence(C12H19NO20S3)n
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeother
experiment.sample.molecule[1].uniprot_sequencenull
experiment.sample.molecule[1].mw15.0
experiment.sample.molecule[1].total_mw15.0
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name20 mM HEPES, 300 mM NaCl, 1 mM TCEP
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameApolipoprotein E4 (methylated) bound to 37 µg/mL heparin
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressBrighton, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Sussex
experiment.contributor[0].affiliation[0].webpagehttp://www.sussex.ac.uk/
experiment.contributor[0].contributor_nameLucas
experiment.contributor[0].contributor_surnameKraft
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-05-03
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames28
experiment.wavelength0.1
experiment.sample_detector_distance4.036
experiment.concentration_minnull
experiment.concentration_max4.0
experiment.sample_volumenull
experiment.flow_ratenull
experiment.s_min0.03
experiment.s_max3.7
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version2.8.4
i0_calibration_standardnull
descriptionApoE4 (methylated) + 37 µg/mL heparin. Molecular weight estimates are inaccurate due to heparin heterogeneity.
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw196.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
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pddf_i0_errornull
guinier_i00.5
guinier_i0_error0.0032
pddf_rg6.58
pddf_rg_errornull
guinier_rg6.392
guinier_rg_error0.061
pddf_dmax22.2
pddf_dmax_errornull
porod_volumenull
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first23
guinier_point_last78
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDG28_datloglog_img.png
symmetrynull
last_modified2024-10-07T16:02:56.037606+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDG28
statussuccess
started_at2026-08-11T15:44:01.275484+00:00
finished_at2026-08-11T15:44:12.084880+00:00
source_last_modified2024-10-07T16:02:56.037606+02:00
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View the complete summary.json source
{
  "code": "SASDG28",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Therapeutic approaches to ApoE",
    "publication": {
      "title": "Therapeutic approaches to ApoE",
      "author_list": "Lucas Kraft",
      "journal": "University of Sussex PhD thesis",
      "doi": null,
      "pmid": null,
      "published_date": "2019 April 25"
    },
    "status": "released",
    "submitted_date": "2019-10-25",
    "released_date": "2021-09-27"
  },
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      },
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          "long_name": "Apolipoprotein E4",
          "short_name": "ApoE4",
          "sequence": "GPHMKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVRGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH",
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          "uniprot_range_last": 317,
          "oligomerization": "tetramer",
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  "tags": [],
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View the complete manifest.json source
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