查看完整 summary.json 原文
{
"code": "SASDG36",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/A",
"project": {
"title": "Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase.",
"publication": {
"title": "Asparagine-84, a regulatory allosteric site residue, helps maintain the quaternary structure of Campylobacter jejuni dihydrodipicolinate synthase.",
"author_list": "Majdi Yazdi M, Saran S, Mrozowich T, Lehnert C, Patel TR, Sanders DAR, Palmer DRJ",
"journal": "J Struct Biol",
"doi": "10.1016/j.jsb.2019.107409",
"pmid": "31678256",
"published_date": "2019 Oct 31"
},
"status": "released",
"submitted_date": "2019-09-29",
"released_date": "2019-11-17"
},
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"experiment": {
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"name": "Pilatus 2M",
"resolution": null
},
"name": "Diamond Light Source",
"city": "Didcot",
"country": "UK",
"beamline_name": "B21",
"beam_geometry": "1 x 5 mm",
"type_of_source": "X-ray synchrotron",
"point_source": null,
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},
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"molecule": [
{
"long_name": "4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant)",
"short_name": "HTPA synthase N84D",
"sequence": "MDKNIIIGAMTALITPFKNGKVDEQSYARLIKRQIENGIDAVVPVGTTGESATLTHEEHRTCIEIAVETCKGTKVKVLAGAGSDATHEAVGLAKFAKEHGADGILSVAPYYNKPTQQGLYEHYKAIAQSVDIPVLLYNVPGRTGCEISTDTIIKLFRDCENIYGVKEASGNIDKCVDLLAHEPRMMLISGEDAINYPILSNGGKGVISVTSNLLPDMISALTHFALDENYKEAKKINDELYNINKILFCESNPIPIKTAMYLAGLIESLEFRLPLCSPSKENFAKIEEVMKKYKIKGF",
"organism": "Campylobacter jejuni",
"uniprot_code": "Q9PPB4",
"uniprot_range_first": 1,
"uniprot_range_last": 298,
"oligomerization": "dimer",
"molecular_type": "protein",
"uniprot_sequence": "MDKNIIIGAMTALITPFKNGKVDEQSYARLIKRQIENGIDAVVPVGTTGESATLTHEEHR\nTCIEIAVETCKGTKVKVLAGAGSNATHEAVGLAKFAKEHGADGILSVAPYYNKPTQQGLY\nEHYKAIAQSVDIPVLLYNVPGRTGCEISTDTIIKLFRDCENIYGVKEASGNIDKCVDLLA\nHEPRMMLISGEDAINYPILSNGGKGVISVTSNLLPDMISALTHFALDENYKEAKKINDEL\nYNINKILFCESNPIPIKTAMYLAGLIESLEFRLPLCSPSKENFAKIEEVMKKYKIKGF",
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"deuteration": null,
"molecule_source": "biological",
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}
],
"buffer": {
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"comment": null,
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"concentration": null,
"pka": null,
"ph": 8.0,
"deuteration": null
},
"purity_method": null,
"name": "4-hydroxy-tetrahydrodipicolinate synthase (N84D mutant)",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": null,
"address": "Lethbridge, AB, Canada",
"full_name": "University of Lethbridge",
"webpage": "http://www.uleth.ca/"
}
],
"contributor_name": "Trushar",
"contributor_surname": "Patel",
"orcid": null
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2017-08-02",
"storage_temperature": null,
"cell_temperature": null,
"exposure_time": 3.0,
"number_of_frames": 619,
"wavelength": 0.1,
"sample_detector_distance": 4.0,
"concentration_min": null,
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"sample_volume": 50.0,
"flow_rate": 0.16,
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},
"fits": [
{
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{
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"software": "DAMMIF",
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"description": "The models displayed in this entry represent: Top, the averaged (DAMFILT) model obtained from the spatial alignment of several individual DAMMIF models (volume and bead occupancy corrected) and; Bottom, and individual DAMMIF model representative and corresponding fit. Additional individual DAMMIF models (in P1 symmetry) are provided in the full entry zip archive.",
"experiment_description": "Synchrotron SAXS data from solutions of N84D mutant 4-hydroxy-tetrahydrodipicolinate synthase in 20 mM Tris-HCl, 150 mM NaCl, pH 8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.16 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column . 619 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
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"pddf_i0": 0.07698,
"pddf_i0_error": 3e-05,
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"porod_volume": 108.0,
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"estimated_volume": null,
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"guinier_point_first": 6,
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"pddf_point_first": null,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDG36_datloglog_img.png",
"symmetry": null,
"last_modified": "2021-04-22T13:41:03.378678+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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