SASDG56

Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2019-12-06T13:07:15.624532+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended

Concentration— – — Buffer / pH25 mM Tris, 150 mM NaCl, 1 mM DTT / 8.0
Experimental temperature20.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.103 nmExposure3.0 s × 600

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
View sequence
MSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPITPPRNSAKAKK
proteinHomo sapiensP621361–330monomerMolecule count 137.512 kDa
Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)
View sequence
MAHHHHHHSAALEVLFQGPGYQDPRRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV
proteinHomo sapiensQ8WUF5621–828monomerMolecule count 125.492 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1500[4]0.0131–0.291235 1/AHas uncertainty columnMissing 00sasbdb/entries/56/sasdg56/source/SASDG56.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)12.4—Å
i0Guinier611.31.33arbitrary
i0P(r)4036.894—arbitrary
mwExperimental65.0—kDa
porod_volumePorod132.4—ų
rgGuinier3.3720.4Å
rgP(r)3.47—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name25 mM Tris, 150 mM NaCl, 1 mM DTTBuffer concentration—
pH8.0Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2019-04-25Storage / measurement temperature7.0 / 20.0
Exposure time3.0Frames600
Wavelength0.103Sample-to-detector distance2.1
SourceX-ray synchrotronDetectorPilatus3 X 2M
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.131 – 2.912Sample volume / flow rate— / 0.5

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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full_entry_zip:source record and ZIP contents (6 items)
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pddf:source record
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:208 field values
Field pathRaw value
codeSASDG56
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleFlexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.
project.publication.titleFlexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.
project.publication.author_listZhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM
project.publication.journalStructure
project.publication.doi10.1016/j.str.2019.07.012
project.publication.pmid31402222
project.publication.published_date2019 Oct 1
project.statusreleased
project.submitted_date2019-10-02
project.released_date2019-11-01
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDG56.dat
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDG56.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG56_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG56_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDG56_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDG56_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDG56.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameSerine/threonine-protein phosphatase PP1-alpha catalytic subunit
experiment.sample.molecule[0].short_namePP-1cα
experiment.sample.molecule[0].sequenceMSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHDLDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPADKNKGKYGQFSGLNPGGRPITPPRNSAKAKK
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP62136
experiment.sample.molecule[0].uniprot_range_first1
experiment.sample.molecule[0].uniprot_range_last330
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTENEIRGLCLKSREIFLSQPILLELEAPLK ICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFL LRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLPIAAIVDEKIFCCHGGLSPDL QSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDVQGWGENDRGVSFTFGAEVVAKFLHKHD LDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDETLMCSFQILKPAD KNKGKYGQFSGLNPGGRPITPPRNSAKAKK
experiment.sample.molecule[0].mw37.512
experiment.sample.molecule[0].total_mw37.512
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.molecule[1].long_nameInhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor)
experiment.sample.molecule[1].short_nameiASPP
experiment.sample.molecule[1].sequenceMAHHHHHHSAALEVLFQGPGYQDPRRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEGITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIFATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFREGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeQ8WUF5
experiment.sample.molecule[1].uniprot_range_first621
experiment.sample.molecule[1].uniprot_range_last828
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMDSEAFQSARDFLDMNFQSLAMKHMDLKQMELDTAAAKVDELTKQLESLWSDSPAPPGPQ AGPPSRPPRYSSSSIPEPFGSRGSPRKAATDGADTPFGRSESAPTLHPYSPLSPKGRPSS PRTPLYLQPDAYGSLDRATSPRPRAFDGAGSSLGRAPSPRPGPGPLRQQGPPTPFDFLGR AGSPRGSPLAEGPQAFFPERGPSPRPPATAYDAPASAFGSSLLGSGGSAFAPPLRAQDDL TLRRRPPKAWNESDLDVAYEKKPSQTASYERLDVFARPASPSLQLLPWRESSLDGLGGTG KDNLTSATLPRNYKVSPLASDRRSDAGSYRRSLGSAGPSGTLPRSWQPVSRIPMPPSSPQ PRGAPRQRPIPLSMIFKLQNAFWEHGASRAMLPGSPLFTRAPPPKLQPQPQPQPQPQSQP QPQLPPQPQTQPQTPTPAPQHPQQTWPPVNEGPPKPPTELEPEPEIEGLLTPVLEAGDVD EGPVARPLSPTRLQPALPPEAQSVPELEEVARVLAEIPRPLKRRGSMEQAPAVALPPTHK KQYQQIISRLFHRHGGPGPGGPEPELSPITEGSEARAGPPAPAPPAPIPPPAPSQSSPPE QPQSMEMRSVLRKAGSPRKARRARLNPLVLLLDAALTGELEVVQQAVKEMNDPSQPNEEG ITALHNAICGANYSIVDFLITAGANVNSPDSHGWTPLHCAASCNDTVICMALVQHGAAIF ATTLSDGATAFEKCDPYREGYADCATYLADVEQSMGLMNSGAVYALWDYSAEFGDELSFR EGESVTVLRRDGPEETDWWWAALHGQEGYVPRNYFGLFPRVKPQRSKV
experiment.sample.molecule[1].mw25.492
experiment.sample.molecule[1].total_mw25.492
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionnull
experiment.sample.buffer.name25 mM Tris, 150 mM NaCl, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
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experiment.sample.buffer.pkanull
experiment.sample.buffer.ph8.0
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameInhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressEdmonton, AB T6G 2R3, Canada
experiment.contributor[0].affiliation[0].full_nameUniversity of Alberta
experiment.contributor[0].affiliation[0].webpage
experiment.contributor[0].contributor_nameHyeong Jin
experiment.contributor[0].contributor_surnameKim
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2019-04-25
experiment.storage_temperature7.0
experiment.cell_temperature20.0
experiment.exposure_time3.0
experiment.number_of_frames600
experiment.wavelength0.103
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_maxnull
experiment.sample_volumenull
experiment.flow_rate0.5
experiment.s_min0.131
experiment.s_max2.912
experiment.total_exposure_timenull
experiment.seccolumn17
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fits[0].softwareMES-FoXS
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fits[0].p_value2.8e-05
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fits[0].description
estimated_volume_methodnull
pddf_softwareScÅtter
pddf_software_version3.1
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw65.0
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i04036.894
pddf_i0_errornull
guinier_i0611.3
guinier_i0_error1.33
pddf_rg3.47
pddf_rg_errornull
guinier_rg3.372
guinier_rg_error0.4
pddf_dmax12.4
pddf_dmax_errornull
porod_volume132.4
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first5
guinier_point_last57
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDG56_datloglog_img.png
symmetrynull
last_modified2019-12-06T13:07:15.624532+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDG56
statussuccess
started_at2026-08-11T16:40:33.167459+00:00
finished_at2026-08-11T16:40:43.930309+00:00
source_last_modified2019-12-06T13:07:15.624532+01:00
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View the complete summary.json source
{
  "code": "SASDG56",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.",
    "publication": {
      "title": "Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.",
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      "doi": "10.1016/j.str.2019.07.012",
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      "published_date": "2019 Oct 1"
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View the complete manifest.json source
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