SASDG62

1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 50 nm filter extrusion

数据类型:SASBDB 实验数据 状态:Published 曲线类型:Single concentration 最后更新:2023-06-21T07:55:24.740692+02:00

DMPC vesicles were extruded through 50 nm pore-diameter filter prior to analysis. Experimental molecular weight estimates do not apply in this instance (in context of the inherent size-distribution, inter-vesicle interactions and multiple contrasts of the sample) and therefore the MW of one DMPC molecule is quoted. The resulting fit to the data and corresponding plot reflects the relative electron density profile perpendicular to the DMPC bilayer of the vesicles, i.e., a bilayer thickness electron density profile, that takes into account the n-component volume-fraction weighted form factors and size distribution of the DMPC vesicle population in solution. Note: For visualization purposes only, the I(s) axis has been arbitrarily scaled in the primary data display (the downloadable .dat and additional files located in the full-entry zip-archive are not scaled accordingly).

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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) vesicles: 50 nm filter extrusion

浓度— – 10.0 缓冲液 / pHwater / 7.0
实验温度30.0 设备 / 束线PETRA III / EMBL P12
波长0.124 nm曝光0.05 s × 20

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
1,2-dimyristoyl-sn-glycero-3-phosphocholine
查看序列
C36H72NO8P
other———–—monomer分子数 10.678 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
12632[3]0.0287918–7.28844 1/nm含误差列缺失 024sasbdb/entries/62/sasdg62/source/SASDG62.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)81.49—nm
i0Guinier5000.0—arbitrary
i0P(r)21190.0—arbitrary
mwExperimental1.0—kDa
rgP(r)31.34—nm

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

拟合 1 · Other LIPMIX

χ²:—CorMap p:0.0单位:1/nm
模型名称 / 类型软件对称性模型 MWPDB 声明来源文件
1—otherOther [static image] ———模型
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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称water缓冲液浓度—
pH7.0添加剂—
缓冲液说明MilliQ Grade water
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2018-07-09储存 / 测量温度30.0 / 30.0
曝光时间0.05帧数20
波长0.124样品-探测器距离3.1
光源X-ray synchrotron探测器Pilatus 6M
机构 / 束线PETRA III / EMBL P12 · DESY; Hamburg, Germany
q 范围0.029 – 7.288样品体积 / 流速— / —

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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curve:来源记录
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

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project.publication.doi10.1107/S1600576720015368
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descriptionDMPC vesicles were extruded through 50 nm pore-diameter filter prior to analysis. Experimental molecular weight estimates do not apply in this instance (in context of the inherent size-distribution, inter-vesicle interactions and multiple contrasts of the sample) and therefore the MW of one DMPC molecule is quoted. The resulting fit to the data and corresponding plot reflects the relative electron density profile perpendicular to the DMPC bilayer of the vesicles, i.e., a bilayer thickness electron density profile, that takes into account the n-component volume-fraction weighted form factors and size distribution of the DMPC vesicle population in solution. Note: For visualization purposes only, the I(s) axis has been arbitrarily scaled in the primary data display (the downloadable .dat and additional files located in the full-entry zip-archive are not scaled accordingly).
experiment_descriptionSynchrotron SAXS data from solutions of DMPC vesicles in water, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10 mg/ml was measured at 30°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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bragg_peak[]
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查看完整 summary.json 原文
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  "experiment_description": "Synchrotron SAXS data from solutions of DMPC vesicles in water, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10 mg/ml was measured at 30°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
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  "last_modified": "2023-06-21T07:55:24.740692+02:00",
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}
查看完整 manifest.json 原文
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