SASDG97

Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2020-01-23T10:37:42.237721+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15

Concentration— – 3.8 Buffer / pH20 mM Tris-HCl, 100 mM NaCl / 7.5
Experimental temperature20.0 Facility / beamlineESRF / BM29
Wavelength0.099 nmExposure1.0 s × 90

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15
View sequence
MYDGDWVFGKRHGTGVYVYADGSRYEGEWVDDKVHGNGACYYTSGNVYTGEWSMGRINGRGVLEYHDGDRYEGEWKDGRMHGKGTYCYSNGDKYEGEWKEDKRHGKGVVVYAAPDGCVSEKYDGEWNEGRMQGWGKYFYADGGVYEGEWVDGRMHGRGTYVFPNGNKYEGEWVEDRKDGYGILLYTNGERYEGYWHLDKAHGKGTLTFLQGDRYVGEWHYGKKHGHGVLSYSNGDTYDGEWRDDDAWGYGVLQYANGCRYEGEWAEDRRHGKGLLVLPDGSSYEGSFAHGKKDGPGKIILKDGSMYIGTWKDGVIVGQGEFRLSENCDLSNPDYSAWSHPQFEK
proteinTrypanosoma bruceiQ587D326–358dimerMolecule count 239.15 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11029[3]0.100721–4.941695 1/nmHas uncertainty columnMissing 05sasbdb/entries/97/sasdg97/source/SASDG97.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)26.0—nm
i0Guinier54.210.13arbitrary
i0P(r)56.63—arbitrary
mwExperimental74.0—kDa
porod_volumePorod187.0—nm³
rgGuinier6.460.57nm
rgP(r)7.31—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

Fit 1 · DAMMIF

χ²: 0.866CorMap p: 0.045772Unit: 1/A

20 runs of Dammif. This model was selected by Damsel.

ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—dummyDAMMIF P260.7—Model Image

Fit 2 · CRYSOL

χ²: 2.201CorMap p: 0.0Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—atomicCustom in-house —74.23—Model Image
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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris-HCl, 100 mM NaClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2017-01-27Storage / measurement temperature— / 20.0
Exposure time1.0Frames90
Wavelength0.099Sample-to-detector distance2.867
SourceX-ray synchrotronDetectorPilatus 1M
Facility / beamlineESRF / BM29 · Grenoble, France
q range0.101 – 4.942Sample volume / flow rate100.0 / 0.5

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:179 field values
Field pathRaw value
codeSASDG97
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStructures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.
project.publication.titleStructures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.
project.publication.author_listSajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K
project.publication.journalPLoS One
project.publication.doi10.1371/journal.pone.0242677
project.publication.pmid33296386
project.publication.published_date2020
project.statusreleased
project.submitted_date2019-10-01
project.released_date2020-01-23
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experiment.instrument.beam_geometry
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experiment.sample.molecule[0].long_nameTrypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15
experiment.sample.molecule[0].short_nametbMORNdelta1
experiment.sample.molecule[0].sequenceMYDGDWVFGKRHGTGVYVYADGSRYEGEWVDDKVHGNGACYYTSGNVYTG EWSMGRINGRGVLEYHDGDRYEGEWKDGRMHGKGTYCYSNGDKYEGEWKE DKRHGKGVVVYAAPDGCVSEKYDGEWNEGRMQGWGKYFYADGGVYEGEWV DGRMHGRGTYVFPNGNKYEGEWVEDRKDGYGILLYTNGERYEGYWHLDKA HGKGTLTFLQGDRYVGEWHYGKKHGHGVLSYSNGDTYDGEWRDDDAWGYG VLQYANGCRYEGEWAEDRRHGKGLLVLPDGSSYEGSFAHGKKDGPGKIIL KDGSMYIGTWKDGVIVGQGEFRLSENCDLSNPDYSAWSHPQFEK
experiment.sample.molecule[0].organismTrypanosoma brucei
experiment.sample.molecule[0].uniprot_codeQ587D3
experiment.sample.molecule[0].uniprot_range_first26
experiment.sample.molecule[0].uniprot_range_last358
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].total_mw78.3
experiment.sample.molecule[0].number_molecules2
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experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMelissa
experiment.contributor[0].contributor_surnameGraewert
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bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDG97
statussuccess
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View the complete summary.json source
{
  "code": "SASDG97",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.",
    "publication": {
      "title": "Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.",
      "author_list": "Sajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K",
      "journal": "PLoS One",
      "doi": "10.1371/journal.pone.0242677",
      "pmid": "33296386",
      "published_date": "2020"
    },
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    "submitted_date": "2019-10-01",
    "released_date": "2020-01-23"
  },
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        {
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View the complete manifest.json source
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