SASDGA7

Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2023-06-21T07:56:39.492710+02:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15

Concentration— – 3.8 Buffer / pH20 mM Tris-HCl, 200 mM NaCl, 2% (w/v) glycerol, 0.5 mM DTT / 8.5
Experimental temperature20.0 Facility / beamlinePETRA III / EMBL P12
Wavelength0.124 nmExposure1.0 s × 60

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN (7-15)
View sequence
MSEKYDGEWNEGRMQGWGKYFYADGGVYEGEWVDGRMHGRGTYVFPNGNKYEGEWVEDRKDGYGILLYTNGERYEGYWHLDKAHGKGTLTFLQGDRYVGEWHYGKKHGHGVLSYSNGDTYDGEWRDDDAWGYGVLQYANGCRYEGEWAEDRRHGKGLLVLPDGSSYEGSFAHGKKDGPGKIILKDGSMYIGTWKDGVIVGQGEFRLSENCDLSNPDYSAWSHPQFEK
proteinTrypanosoma bruceiQ587D3143–358dimerMolecule count 225.927 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
11092[3]0.0795628–3.05221 1/nmHas uncertainty columnMissing 036sasbdb/entries/a7/sasdga7/source/SASDGA7.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)15.5—nm
i0Guinier2829.06.0arbitrary
i0P(r)2876.0—arbitrary
mwExperimental46.0—kDa
mwPorod35.0—kDa
porod_volumePorod56.0—nm³
rgGuinier4.08—nm
rgP(r)4.379—nm

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

Fit 1 · CRYSOL

χ²: 1.16CorMap p: 0.032524Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—atomicCustom in-house —44.69—Model Image
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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM Tris-HCl, 200 mM NaCl, 2% (w/v) glycerol, 0.5 mM DTTBuffer concentration—
pH8.5Additives—
Buffer descriptionSEC-SAXS buffer
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2016-06-11Storage / measurement temperature— / 20.0
Exposure time1.0Frames60
Wavelength0.124Sample-to-detector distance3.0
SourceX-ray synchrotronDetectorPilatus 2M
Facility / beamlinePETRA III / EMBL P12 · DESY; Hamburg, Germany
q range0.08 – 3.052Sample volume / flow rate100.0 / 0.5

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:156 field values
Field pathRaw value
codeSASDGA7
statusPublished
type_of_curveSEC-SAS
angular_unit1/nm
project.titleStructures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.
project.publication.titleStructures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.
project.publication.author_listSajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K
project.publication.journalPLoS One
project.publication.doi10.1371/journal.pone.0242677
project.publication.pmid33296386
project.publication.published_date2020
project.statusreleased
project.submitted_date2019-10-01
project.released_date2020-01-23
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experiment.instrument.cityDESY; Hamburg
experiment.instrument.countryGermany
experiment.instrument.beamline_nameEMBL P12
experiment.instrument.beam_geometrynull
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experiment.instrument.point_sourcenull
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experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
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experiment.sample.molecule[0].long_nameTrypanosoma brucei Membrane Occupation and Recognition Nexus MORN (7-15)
experiment.sample.molecule[0].short_nametbMORNdelta6
experiment.sample.molecule[0].sequenceMSEKYDGEWNEGRMQGWGKYFYADGGVYEGEWVDGRMHGRGTYVFPNGNK YEGEWVEDRKDGYGILLYTNGERYEGYWHLDKAHGKGTLTFLQGDRYVGE WHYGKKHGHGVLSYSNGDTYDGEWRDDDAWGYGVLQYANGCRYEGEWAED RRHGKGLLVLPDGSSYEGSFAHGKKDGPGKIILKDGSMYIGTWKDGVIVG QGEFRLSENCDLSNPDYSAWSHPQFEK
experiment.sample.molecule[0].organismTrypanosoma brucei
experiment.sample.molecule[0].uniprot_codeQ587D3
experiment.sample.molecule[0].uniprot_range_first143
experiment.sample.molecule[0].uniprot_range_last358
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeprotein
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experiment.sample.molecule[0].mw25.927
experiment.sample.molecule[0].total_mw51.854
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionnull
experiment.sample.buffer.name20 mM Tris-HCl, 200 mM NaCl, 2% (w/v) glycerol, 0.5 mM DTT
experiment.sample.buffer.concentration_unitnull
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experiment.sample.buffer.additivenull
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experiment.sample.contrastnull
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experiment.contributor[0].affiliation[0].short_nameEMBL-Hamburg
experiment.contributor[0].affiliation[0].addressNotkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany
experiment.contributor[0].affiliation[0].full_nameEuropean Molecular Biology Laboratory (EMBL) - Hamburg outstation
experiment.contributor[0].affiliation[0].webpagehttp://www.embl-hamburg.de/index.php
experiment.contributor[0].contributor_nameMelissa
experiment.contributor[0].contributor_surnameGraewert
experiment.contributor[0].orcidnull
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experiment.seccolumn1
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experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw46.0
experimental_mw_errornull
guinier_i0_mwnull
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porod_mw_errornull
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guinier_point_last88
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symmetrynull
last_modified2023-06-21T07:56:39.492710+02:00
bragg_peak[]
manifest.json:36 field values
Field pathRaw value
codeSASDGA7
statussuccess
started_at2026-08-11T15:25:18.696397+00:00
finished_at2026-08-11T15:25:32.595648+00:00
source_last_modified2023-06-21T07:56:39.492710+02:00
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View the complete summary.json source
{
  "code": "SASDGA7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/nm",
  "project": {
    "title": "Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.",
    "publication": {
      "title": "Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.",
      "author_list": "Sajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K",
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View the complete manifest.json source
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