Data type: SASBDB experimental dataStatus: PublishedCurve type: Single concentrationLast updated: 2023-06-21T07:55:24.740692+02:00
DPPC vesicles were extruded through 100 nm pore-diameter filter prior to analysis. Experimental molecular weight estimates do not apply in this instance (in context of the inherent size-distribution, inter-vesicle interactions and multiple contrasts of the sample) and therefore the MW of one DPPC molecule is quoted. The resulting fit to the data and corresponding plot reflects the relative electron density profile perpendicular to the DPPC bilayer of the vesicles, i.e., a bilayer thickness electron density profile, that takes into account the n-component volume-fraction weighted form factors and size distribution of the DPPC vesicle population in solution. Note: For visualization purposes only, the I(s) axis has been arbitrarily scaled in the primary data display (the downloadable .dat and additional files located in the full-entry zip-archive are not scaled accordingly).
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1. Samples, Components and Experimental Conditions Sample & Experiment
5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
Synchrotron SAXS data from solutions of DPCC vesicles in water, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10 mg/ml was measured at 45°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Related Publication
Title
Restoring structural parameters of lipid mixtures from small-angle X-ray scattering data
Authors
Konarev P, Gruzinov A, Mertens H, Svergun D
DOI / PMID
10.1107/S1600576720015368 / —
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6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
DPPC vesicles were extruded through 100 nm pore-diameter filter prior to analysis. Experimental molecular weight estimates do not apply in this instance (in context of the inherent size-distribution, inter-vesicle interactions and multiple contrasts of the sample) and therefore the MW of one DPPC molecule is quoted. The resulting fit to the data and corresponding plot reflects the relative electron density profile perpendicular to the DPPC bilayer of the vesicles, i.e., a bilayer thickness electron density profile, that takes into account the n-component volume-fraction weighted form factors and size distribution of the DPPC vesicle population in solution. Note: For visualization purposes only, the I(s) axis has been arbitrarily scaled in the primary data display (the downloadable .dat and additional files located in the full-entry zip-archive are not scaled accordingly).
experiment_description
Synchrotron SAXS data from solutions of DPCC vesicles in water, pH 7 were collected on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10 mg/ml was measured at 45°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
