Wild-type ELP in 6 M urea measured at 13428 eV. SAXS data measured at several different energies spanning the K-absorbtion edge of bromine are made available in the full entry zip archive.
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1. 样品、组分与实验条件 Sample & Experiment
样品 1 · Wild-type Elastin-like Polypeptide (ELP) in 6 M Urea
浓度
— – 20.0
缓冲液 / pH
phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT / 7.4
Synchrotron SAXS data from solutions of wild-type ELP in phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 20 mg/ml was injected at a 0.35 ml/min flow rate onto a column at 20°C. 2400 successive 0.250 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
相关论文
标题
A genetically encoded anomalous SAXS ruler to probe the dimensions of intrinsically disordered proteins.
作者
Yu M, Gruzinov AY, Ruan H, Scheidt T, Chowdhury A, Giofrè S, Mohammed ASA, Caria J, Sauter PF, Svergun DI, Lemke EA
Wild-type ELP in 6 M urea measured at 13428 eV. SAXS data measured at several different energies spanning the K-absorbtion edge of bromine are made available in the full entry zip archive.
experiment_description
Synchrotron SAXS data from solutions of wild-type ELP in phosphate buffered saline containing 6 M urea, 0.3 M KCl, 5 mM DTT, pH 7.4 were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 20 mg/ml was injected at a 0.35 ml/min flow rate onto a column at 20°C. 2400 successive 0.250 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
