Polyhistidine tagged Cell division protein SepF C-terminal domain (2 mg/mL) from Mycobacterium tuberculosis

{
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  "angular_unit": "1/nm",
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    "title": "SAXS data of Mycobacterium tuberculosis SepF-C-terminal domain",
    "publication": null,
    "status": "released",
    "submitted_date": "2024-02-13",
    "released_date": "2026-02-13"
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  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Pilatus3 2M",
        "resolution": 0.172
      },
      "name": "ESRF",
      "city": "Grenoble",
      "country": "France",
      "beamline_name": "BM29",
      "beam_geometry": "point",
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
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      "line_collimation_integrationwidth": null,
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      "beam_profile_al": null
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      "molecule": [
        {
          "long_name": "Cell division protein SepF",
          "short_name": "SepF",
          "sequence": "MGHHHHHHENFYQGDGHPLSKITTLRPKDYSEARTIGERFRDGSPVIMDLVSMDNADAKRLVDFAAGLAFALRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAYQ",
          "organism": "Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)",
          "uniprot_code": "P9WGJ5",
          "uniprot_range_first": 123,
          "uniprot_range_last": 218,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSTLHKVKAYFGMAPMEDYDDEYYDDRAPSRGYARPRFDDDYGRYDGRDYDDARSDSRGD\nLRGEPADYPPPGYRGGYADEPRFRPREFDRAEMTRPRFGSWLRNSTRGALAMDPRRMAMM\nFEDGHPLSKITTLRPKDYSEARTIGERFRDGSPVIMDLVSMDNADAKRLVDFAAGLAFAL\nRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAYQ",
          "mw": 12.402,
          "total_mw": 24.804,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "C-terminal domain of cell division protein SepF. The protein construct used for SAXS contains a N-terminal polyhistidine tag (MGHHHHHHENFYQG)."
        }
      ],
      "buffer": {
        "name": "Tris 25 mM, 100 mM NaCl, 10% glycerol, 1 mM EDTA",
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        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.5,
        "deuteration": null
      },
      "purity_method": null,
      "name": "Polyhistidine tagged Cell division protein SepF C-terminal domain (2 mg/mL) from Mycobacterium tuberculosis",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": "CSIR",
            "address": "Chandigarh, India",
            "full_name": "CSIR-Institute of Microbial Technology",
            "webpage": "http://www.imtech.res.in/"
          }
        ],
        "contributor_name": "Jagrity",
        "contributor_surname": "Choudhury",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-11-25",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 0.5,
    "number_of_frames": 10,
    "wavelength": 0.1,
    "sample_detector_distance": 2.9,
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    "concentration_max": 2.0,
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    "flow_rate": null,
    "s_min": 0.038,
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    "seccolumn": null
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "Synchrotron SAXS data from solutions of cell division protein SepF (C-terminal domain) in 25 mM Tris, 100 mM NaCl, 10% glycerol, 1 mM EDTA, pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 20°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 23.0,
  "experimental_mw_error": null,
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  "guinier_rg_error": 0.022,
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  "pddf_dmax_error": null,
  "porod_volume": 41.7,
  "porod_volume_error": null,
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  "guinier_point_first": 18,
  "guinier_point_last": 106,
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  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2026-02-22T17:36:24.118611+01:00",
  "bragg_peak": []
}

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