SASDV23

N-terminal domain construct of hsGEFH1_28-582aa fit to various models

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-12-11T10:48:36.903457+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · N-terminal domain construct of hsGEFH1_28-582aa fit to various models

Concentration— – — Buffer / pH20mM Tris-Cl, 150mM NaCl, 1mM DTT / 7.5
Experimental temperature— Facility / beamlineDiamond Light Source / B21
Wavelength— nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Isoform 1 of Rho guanine nucleotide exchange factor 2
View sequence
MKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIKHHHHHH
proteinHomo sapiensQ9297428–582monomerMolecule count 164.363 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1485[3]0.01141–0.2045 1/AHas uncertainty columnMissing 00sasbdb/entries/23/sasdv23/source/SASDV23.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
i0Guinier0.0208036—arbitrary
mwExperimental60.0—kDa
rgGuinier3.7462.945Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM Tris-Cl, 150mM NaCl, 1mM DTTBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2021-02-19Storage / measurement temperature— / —
Exposure time—Frames—
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.114 – 2.045Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

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sascifsasbdb/entries/23/sasdv23/source/SASDV23.sascifnot_available——DownloadView source fileSource site
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full_entry_zip:source record and ZIP contents (7 items)
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pddf:source record
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:202 field values
Field pathRaw value
codeSASDV23
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleStructural basis of microtubule-mediated signal transduction.
project.publication.titleStructural basis of microtubule-mediated signal transduction.
project.publication.author_listChoi SR, Blum TB, Giono M, Roy B, Vakonakis I, Schmid D, Oelgarth N, Ranganathan A, Gossert AD, Shivashankar GV, Zippelius A, Steinmetz MO
project.publication.journalCell
project.publication.doi10.1016/j.cell.2025.11.011
project.publication.pmid41365297
project.publication.published_date2025 Dec 8
project.statusreleased
project.submitted_date2024-06-18
project.released_date2025-12-11
pddf_datanull
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experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.instrument.sample_path_lengthnull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameIsoform 1 of Rho guanine nucleotide exchange factor 2
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMKEAKDARYTNGHLFTTISVSGMTMCYACNKSITAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERPSSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNMRNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIKHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ92974
experiment.sample.molecule[0].uniprot_range_first28
experiment.sample.molecule[0].uniprot_range_last582
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSRIESLTRARIDRSRELASKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSI TAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERP SSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNM RNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVI YELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLER RRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDK RFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALG LVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLL WKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFL ISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRIKMELQQK DRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRGERLLQDAIR EVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSD SSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRA NSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLR ESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPP QPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHS PRDFTRMQDIPEETESRDGEAVASES
experiment.sample.molecule[0].mw64.363
experiment.sample.molecule[0].total_mw64.363
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionN-terminal construct of GEFH1 encompassing the C1, DH, and PH domains. C-terminal hexahistidine tag
experiment.sample.buffer.name20mM Tris-Cl, 150mM NaCl, 1mM DTT
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experiment.sample.buffer.commentnull
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experiment.sample.nameN-terminal domain construct of hsGEFH1_28-582aa fit to various models
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
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experiment.sample.mixturenull
experiment.contributor[]
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experiment.concentration_unitnull
experiment.date2021-02-19
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intensities_log_log_plotSASDV23_datloglog_img.png
symmetrynull
last_modified2025-12-11T10:48:36.903457+01:00
bragg_peak[]
manifest.json:31 field values
Field pathRaw value
codeSASDV23
statussuccess
started_at2026-08-11T16:35:24.989514+00:00
finished_at2026-08-11T16:35:33.989872+00:00
source_last_modified2025-12-11T10:48:36.903457+01:00
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View the complete summary.json source
{
  "code": "SASDV23",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural basis of microtubule-mediated signal transduction.",
    "publication": {
      "title": "Structural basis of microtubule-mediated signal transduction.",
      "author_list": "Choi SR, Blum TB, Giono M, Roy B, Vakonakis I, Schmid D, Oelgarth N, Ranganathan A, Gossert AD, Shivashankar GV, Zippelius A, Steinmetz MO",
      "journal": "Cell",
      "doi": "10.1016/j.cell.2025.11.011",
      "pmid": "41365297",
      "published_date": "2025 Dec 8"
    },
    "status": "released",
    "submitted_date": "2024-06-18",
    "released_date": "2025-12-11"
  },
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View the complete manifest.json source
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