SASDV33

N-terminal domain construct of hsGEFH1_190-582aa fit to DAMMIF and Alphafold2 models

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2025-12-11T10:48:35.751806+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · N-terminal domain construct of hsGEFH1_190-582aa fit to DAMMIF and Alphafold2 models

Concentration— – — Buffer / pH20mM Tris-Cl, 150mM NaCl, 1mM DTT / 7.5
Experimental temperature— Facility / beamlineDiamond Light Source / B21
Wavelength— nmExposure— s × —

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Isoform 1 of Rho guanine nucleotide exchange factor 2
View sequence
MLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIKHHHHHH
proteinHomo sapiensQ92974190–582monomerMolecule count 146.68 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1427[3]0.01016–0.2385 1/AHas uncertainty columnMissing 023sasbdb/entries/33/sasdv33/source/SASDV33.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
i0Guinier0.00709158—arbitrary
mwExperimental41.0—kDa
rgGuinier5.90912.428Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

Fit 2 · DAMMIF

χ²: 5.768CorMap p: 0.8163Unit: 1/A
ModelName / TypeSoftwareSymmetryModel MWPDB DeclarationSource file
1—dummyDAMMIF DAMFILT 5.0 (r13469)—36.793—Model Image Log
2—dummyDAMMIF dammif (r13469)—36.8—Model Image
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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20mM Tris-Cl, 150mM NaCl, 1mM DTTBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2021-02-19Storage / measurement temperature— / —
Exposure time—Frames—
Wavelength—Sample-to-detector distance—
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.102 – 2.385Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot

Downloadable Files

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sascifsasbdb/entries/33/sasdv33/source/SASDV33.sascifnot_available——DownloadView source fileSource site
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full_entry_zip:source record and ZIP contents (6 items)
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sascif:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:192 field values
Field pathRaw value
codeSASDV33
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleStructural basis of microtubule-mediated signal transduction.
project.publication.titleStructural basis of microtubule-mediated signal transduction.
project.publication.author_listChoi SR, Blum TB, Giono M, Roy B, Vakonakis I, Schmid D, Oelgarth N, Ranganathan A, Gossert AD, Shivashankar GV, Zippelius A, Steinmetz MO
project.publication.journalCell
project.publication.doi10.1016/j.cell.2025.11.011
project.publication.pmid41365297
project.publication.published_date2025 Dec 8
project.statusreleased
project.submitted_date2024-06-18
project.released_date2025-12-11
pddf_datanull
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pddf_plotnull
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experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIsoform 1 of Rho guanine nucleotide exchange factor 2
experiment.sample.molecule[0].short_namenull
experiment.sample.molecule[0].sequenceMLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIKHHHHHH
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeQ92974
experiment.sample.molecule[0].uniprot_range_first190
experiment.sample.molecule[0].uniprot_range_last582
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMSRIESLTRARIDRSRELASKTREKEKMKEAKDARYTNGHLFTTISVSGMTMCYACNKSI TAKEALICPTCNVTIHNRCKDTLANCTKVKQKQQKAALLKNNTALQSVSLRSKTTIRERP SSAIYPSDSFRQSLLGSRRGRSSLSLAKSVSTTNIAGHFNDESPLGLRRILSQSTDSLNM RNRTLSVESLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVI YELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLER RRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDK RFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALG LVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLL WKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFL ISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIETEDEAYLRRIKMELQQK DRALVELLREKVGLFAEMTHFQAEEDGGSGMALPTLPRGLFRSESLESPRGERLLQDAIR EVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSD SSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRA NSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLR ESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPP QPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHS PRDFTRMQDIPEETESRDGEAVASES
experiment.sample.molecule[0].mw46.68
experiment.sample.molecule[0].total_mw46.68
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20mM Tris-Cl, 150mM NaCl, 1mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
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experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameN-terminal domain construct of hsGEFH1_190-582aa fit to DAMMIF and Alphafold2 models
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
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experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_namePaul Scherrer Institut
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameSung Ryul
experiment.contributor[0].contributor_surnameChoi
experiment.contributor[0].orcidhttps://orcid.org/0000-0002-4240-2150
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2021-02-19
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experiment.cell_temperaturenull
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experiment.total_exposure_timenull
experiment.seccolumnnull
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fits[0].softwareFoXS
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fits[0].p_value0.8163
fits[0].fit_residual_plotSASDV33_fit1_fitresiduals_img.png
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tags[]
intensity_unitarbitrary
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pddf_dmaxnull
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i0_calibration_standard_datanull
intensities_log_log_plotSASDV33_datloglog_img.png
symmetrynull
last_modified2025-12-11T10:48:35.751806+01:00
bragg_peak[]
manifest.json:31 field values
Field pathRaw value
codeSASDV33
statussuccess
started_at2026-08-11T16:24:42.407266+00:00
finished_at2026-08-11T16:24:49.207046+00:00
source_last_modified2025-12-11T10:48:35.751806+01:00
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View the complete summary.json source
{
  "code": "SASDV33",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural basis of microtubule-mediated signal transduction.",
    "publication": {
      "title": "Structural basis of microtubule-mediated signal transduction.",
      "author_list": "Choi SR, Blum TB, Giono M, Roy B, Vakonakis I, Schmid D, Oelgarth N, Ranganathan A, Gossert AD, Shivashankar GV, Zippelius A, Steinmetz MO",
      "journal": "Cell",
      "doi": "10.1016/j.cell.2025.11.011",
      "pmid": "41365297",
      "published_date": "2025 Dec 8"
    },
    "status": "released",
    "submitted_date": "2024-06-18",
    "released_date": "2025-12-11"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDV33.dat",
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      "country": "UK",
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      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
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    "sample": {
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          "long_name": "Isoform 1 of Rho guanine nucleotide exchange factor 2",
          "short_name": null,
          "sequence": "MLIDEAEVIYSELMSDFEMDEKDFAADSWSLAVDSSFLQQHKKEVMKQQDVIYELIQTELHHVRTLKIMTRLFRTGMLEELHLEPGVVQGLFPCVDELSDIHTRFLSQLLERRRQALCPGSTRNFVIHRLGDLLISQFSGPSAEQMCKTYSEFCSRHSKALKLYKELYARDKRFQQFIRKVTRPAVLKRHGVQECILLVTQRITKYPLLISRILQHSHGIEEERQDLTTALGLVKELLSNVDEGIYQLEKGARLQEIYNRMDPRAQTPVPGKGPFGREELLRRKLIHDGCLLWKTATGRFKDVLVLLMTDVLVFLQEKDQKYIFPTLDKPSVVSLQNLIVRDIANQEKGMFLISAAPPEMYEVHTASRDDRSTWIRVIQQSVRTCPSREDFPLIKHHHHHH",
          "organism": "Homo sapiens",
          "uniprot_code": "Q92974",
          "uniprot_range_first": 190,
          "uniprot_range_last": 582,
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View the complete manifest.json source
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