查看完整 summary.json 原文
{
"code": "SASDV54",
"status": "Published",
"type_of_curve": "SEC-SAS",
"angular_unit": "1/nm",
"project": {
"title": "Structural-function relationship of YdaS, a Cro-type repressor in the cryptic prophage CP-933P from Escherichia coli O157:H7",
"publication": null,
"status": "released",
"submitted_date": "2024-07-01",
"released_date": "2024-07-30"
},
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"name": "ESRF",
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"country": "France",
"beamline_name": "BM29",
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"type_of_source": "X-ray synchrotron",
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{
"long_name": "Phage antirepressor protein Cro",
"short_name": "YdaS protein",
"sequence": "MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRALMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDSGSSHHHHHH",
"organism": "Escherichia coli O157:H7",
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"oligomerization": "dimer",
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"number_molecules": 2,
"complex_state": false,
"deuteration": null,
"molecule_source": "biological",
"molecule_description": "Phage antirepressor protein Cro. The protein construct used for SAXS contains an additional non-native polyhistidine tag at the C-terminus (GSSHHHHHH)."
},
{
"long_name": "IR1 22 base pair dsDNA",
"short_name": "IR1",
"sequence": "TTGTTTAGTGATTACTAAAGAT\r\nAACAAATCACTAATGATTTCTA",
"organism": null,
"uniprot_code": null,
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"oligomerization": "monomer",
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"uniprot_sequence": "",
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"number_molecules": 1,
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"deuteration": null,
"molecule_source": "biological",
"molecule_description": ""
}
],
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"concentration_unit": null,
"comment": null,
"additive": null,
"concentration": null,
"pka": null,
"ph": 5.5,
"deuteration": null
},
"purity_method": null,
"name": "YdaS, a cro-type repressor from Escherichai coli O157:H7 cryptic prophage CP-933 bound to a 22 base pair dsDNA.",
"ext_coefficient": null,
"contrast": null,
"specific_vol": null,
"dry_vol": null,
"absorbption": null,
"deuteration": null,
"mixture": null
},
"contributor": [
{
"affiliation": [
{
"short_name": "VUB",
"address": "Pleinlaan 2 1050 Brussel ",
"full_name": "Vrije Universiteit Brussel",
"webpage": "http://www.vub.ac.be/"
}
],
"contributor_name": "Marusa",
"contributor_surname": "Prolic Kalinsek",
"orcid": "https://orcid.org/0000-0003-4175-1858"
}
],
"concentration_method": null,
"concentration_unit": null,
"date": "2021-04-13",
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"total_exposure_time": null,
"seccolumn": 34
},
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"software": "Other [static image]",
"pdb_link": [],
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"type_of_model": "other",
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"comment": null,
"user": 9
}
],
"fit_unit": "1/A",
"fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDV54_fit1_fixed_fit_img.png",
"software": "Other",
"chi_square_value": 1.2621,
"p_value": 0.0148,
"fit_residual_plot": "SASDV54_fit1_fitresiduals_img.png",
"fit_data": "https://www.sasbdb.org/media/fitting_files/SASDV54_fit1.saxs",
"fit_log": null,
"software_version": "calcSAXS",
"description": ""
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"description": "The atomic coordinates of the full model ensemble are made available in the full entry zip archive. X-ray exposure time = UNKNOWN. Number of sample data frames = UNKNOWN.",
"experiment_description": "Synchrotron SAXS data from solutions of YdaS, a cro-type repressor from Escherichai coli O157:H7 cryptic prophage CP-933 bound to a 22 base pair dsDNA in 20 mM sodium acetate, 150 mM NaCl, 1 mM TCEP, pH 5.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 15 mg/ml was injected at a 0.16 ml/min flow rate onto a Agilent AdvanceBio SEC 300Å, 4.6 x 150 mm column at 15°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
"tags": [],
"intensity_unit": "arbitrary",
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"guinier_i0_mw_error": null,
"porod_mw": null,
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"pddf_i0": 140.7,
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"porod_volume": 52.95,
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"estimated_volume": null,
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"guinier_point_first": 21,
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"pddf_point_first": null,
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"i0_calibration_standard_data": null,
"intensities_log_log_plot": "SASDV54_datloglog_img.png",
"symmetry": null,
"last_modified": "2024-07-30T18:06:40.919061+02:00",
"bragg_peak": []
}查看完整 manifest.json 原文
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"status": "success",
"started_at": "2026-08-11T14:14:25.042298+00:00",
"finished_at": "2026-08-11T14:14:34.357054+00:00",
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