Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%

{
  "code": "SASDV59",
  "status": "Published",
  "type_of_curve": "Single concentration",
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    "title": "Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation",
    "publication": {
      "title": "Structural dynamics of IDR interactions in human SFPQ and implications for liquid–liquid phase separation",
      "author_list": "Koning H, Lai V, Sethi A, Chakraborty S, Ang C, Fox A, Duff A, Whitten A, Marshall A, Bond C",
      "journal": "Acta Crystallographica Section D Structural Biology",
      "doi": "10.1107/S2059798325005303",
      "pmid": null,
      "published_date": "2025 Jun 27"
    },
    "status": "released",
    "submitted_date": "2024-09-18",
    "released_date": "2025-06-29"
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  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV59_pofr_img.png",
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  "experiment": {
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      "detector": {
        "type": null,
        "name": "ORDELA/21000N",
        "resolution": 5.1
      },
      "name": "Australian Centre for Neutron Scattering (ANSTO)",
      "city": "Lucas Heights, Sydney",
      "country": "Australia",
      "beamline_name": "Quokka - Small Angle Neutron Scattering",
      "beam_geometry": null,
      "type_of_source": "neutron source",
      "point_source": null,
      "line_collimation": null,
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      "line_collimation_integrationwidth": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Splicing factor, proline- and glutamine-rich",
          "short_name": "SFPQ cysteine dimer",
          "sequence": "GAMSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPIPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPGVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGEPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLFVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGRQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFASKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLMRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRMGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGSDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF",
          "organism": "Homo sapiens",
          "uniprot_code": "P23246",
          "uniprot_range_first": 214,
          "uniprot_range_last": 707,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MSRDRFRSRGGGGGGFHRRGGGGGRGGLHDFRSPPPGMGLNQNRGPMGPGPGQSGPKPPI\nPPPPPHQQQQQPPPQQPPPQQPPPHQPPPHPQPHQQQQPPPPPQDSSKPVVAQGPGPAPG\nVGSAPPASSSAPPATPPTSGAPPGSGPGPTPTPPPAVTSAPPGAPPPTPPSSGVPTTPPQ\nAGGPPPPPAAVPGPGPGPKQGPGPGGPKGGKMPGGPKPGGGPGLSTPGGHPKPPHRGGGE\nPRGGRQHHPPYHQQHHQGPPPGGPGGRSEEKISDSEGFKANLSLLRRPGEKTYTQRCRLF\nVGNLPADITEDEFKRLFAKYGEPGEVFINKGKGFGFIKLESRALAEIAKAELDDTPMRGR\nQLRVRFATHAAALSVRNLSPYVSNELLEEAFSQFGPIERAVVIVDDRGRSTGKGIVEFAS\nKPAARKAFERCSEGVFLLTTTPRPVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRF\nAQHGTFEYEYSQRWKSLDEMEKQQREQVEKNMKDAKDKLESEMEDAYHEHQANLLRQDLM\nRRQEELRRMEELHNQEMQKRKEMQLRQEEERRRREEEMMIRQREMEEQMRRQREESYSRM\nGYMDPRERDMRMGGGGAMNMGDPYGSGGQKFPPLGGGGGIGYEANPGVPPATMSGSMMGS\nDMRTERFGQGGAGPVGGQGPRGMGPGTPAGYGRGREEYEGPNKKPRF",
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          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "R542C mutation causes monomers of SFPQ to dimerise via the coiled-coil domain (Lee et al, 2015)"
        }
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      "buffer": {
        "name": "150 mM KCl, 0.075% glycerol,  20 mM HEPES, 0.5 mM DTT,",
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        "comment": "SANS contrast matching buffer",
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.4,
        "deuteration": 95.0
      },
      "purity_method": null,
      "name": "Human Splicing factor, proline- and glutamine-rich protein 5% SFPQ and 95% dSFPQ at a D2O matchpoint of 95%",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "The University of Western Australia",
            "webpage": null
          }
        ],
        "contributor_name": "Heidar",
        "contributor_surname": "Koning",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-01-20",
    "storage_temperature": 25.0,
    "cell_temperature": 25.0,
    "exposure_time": null,
    "number_of_frames": null,
    "wavelength": 0.6,
    "sample_detector_distance": 1.3,
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    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.082,
    "s_max": 4.352,
    "total_exposure_time": null,
    "seccolumn": null
  },
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  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": "SANS experiment indicating dimer partner exchange between deuterated and protiated monomers of full-length SFPQ. Contrast-matching has ensured signal is coming from protiated monomers of full-length SFPQ.\r\n\r\nAdditional details are available in the manuscript: Structural dynamics of IDR interactions in human SFPQ and implications for liquid-liquid phase separation.",
  "experiment_description": null,
  "tags": [],
  "intensity_unit": "arbitrary",
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  "experimental_mw_error": null,
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  "guinier_i0_mw_error": null,
  "porod_mw": 113.0,
  "porod_mw_error": null,
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  "guinier_i0_error": null,
  "pddf_rg": 6.64,
  "pddf_rg_error": null,
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  "guinier_rg_error": 0.418,
  "pddf_dmax": 22.0,
  "pddf_dmax_error": null,
  "porod_volume": 181.0,
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  "estimated_volume_error": null,
  "guinier_point_first": 7,
  "guinier_point_last": 19,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
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  "symmetry": null,
  "last_modified": "2025-07-03T11:05:27.816508+02:00",
  "bragg_peak": []
}

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