The light-adapted AsLOV2 domain (Avena sativa, Oat)

{
  "code": "SASDV65",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.",
    "publication": {
      "title": "Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.",
      "author_list": "Kim C, Yun SR, Lee SJ, Kim SO, Lee H, Choi J, Kim JG, Kim TW, You S, Kosheleva I, Noh T, Baek J, Ihee H",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-024-51461-z",
      "pmid": "39143073",
      "published_date": "2024 Aug 14"
    },
    "status": "released",
    "submitted_date": "2024-07-11",
    "released_date": "2024-07-27"
  },
  "pddf_data": null,
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDV65.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV65_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV65_kratky_img.png",
  "pddf_plot": null,
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV65_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDV65.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": "CCD",
        "name": "Rayonix SX165",
        "resolution": 15.0
      },
      "name": "Pohang Accelerator Laboratory",
      "city": "Pohang",
      "country": "South Korea",
      "beamline_name": "4C",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "non-specific serine/threonine protein kinase",
          "short_name": null,
          "sequence": "FLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENIDEAAKEL",
          "organism": "Avena sativa",
          "uniprot_code": "O49003",
          "uniprot_range_first": 404,
          "uniprot_range_last": 546,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MASKGAGGGGGGHEEPQRPKQQLPRDSRGSLEVFNPSSSSAAVEPPSAFRPAARSASPFI\nEEATGGIEDVGKATQRAAEWGLVLQTNEQTGRPQGVSARSSGGGGSARSSSDDKAVAGAI\nPRVSEELRAALSAFQQTFVVSDASRPGHPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSG\nTDPAEIAKIRQALANGSNYCGRVLNYKKDGTAFWNLLTIAPIKDEEGRVLKFIGMQVEVS\nKYTEGNKDTVVRPNGLPESLIKYDARQKDQARSSVSELLLAIKNPRSLSESTNSTFKRKS\nQESVGALTGDRPGKRSSESGSRRNSKSGARTSLQKISEVPERGSKSRKSGLYSLMSLLGM\nGPGNIEKDMLKPRDEDPLLDSDDERPESFDDELRRKEMRRGIDLATTLERIEKNFVITDP\nRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATVRKIRDAIDNQTEVTVQL\nINYTKSGKKFWNLFHLQPMRDQKGDVQYFIGVQLDGTEHVRDAAEREGVMLIKKTAENID\nEAAKELPDANLRPEDLWANHSKVVLPKPHMKDSASWRAIQKVLEGGENIDLKHFRPVKPL\nGSGDTGSVHLVELLNTGEYFAMKAMDKNVMLNRNKVHRANAEREILDMLDHPFLPTLYAS\nFQTKTHICLITDYYPGGELFLLLDRQPLKVLREDAVRFYAAEVVIALEYLHCQGIIYRDL\nKPENILLHRDGHISLTDFDLSCLTSCRPQVFLPEEANKKSRRKSRSSPIFFAEPMRASNS\nFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKDIRF\nPASISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKLDAP\nLFPDDTDKGMGDAAAADTHTDMF",
          "mw": 16.645,
          "total_mw": 33.29,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "Tandem PAS-PAC domains."
        }
      ],
      "buffer": {
        "name": "20 mM Tris, 200 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 7.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "The light-adapted AsLOV2 domain (Avena sativa, Oat)",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Korea Advanced Institute of Science and Technology",
            "webpage": null
          }
        ],
        "contributor_name": "Hyotcherl",
        "contributor_surname": "Ihee",
        "orcid": "https://orcid.org/0000-0003-0397-5965"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2021-05-15",
    "storage_temperature": 24.0,
    "cell_temperature": 24.0,
    "exposure_time": 10.0,
    "number_of_frames": 50,
    "wavelength": 0.0734,
    "sample_detector_distance": 3.0,
    "concentration_min": null,
    "concentration_max": 24.98,
    "sample_volume": null,
    "flow_rate": null,
    "s_min": 0.252,
    "s_max": 5.999,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [],
  "estimated_volume_method": null,
  "pddf_software": null,
  "pddf_software_version": null,
  "i0_calibration_standard": null,
  "description": "The SAXS data of AsLOV2 in the light-adapted state were measured where the target sample was continuously illuminated at a wavelength of 455 nm.",
  "experiment_description": "Synchrotron SAXS data from solutions of light-adapted AsLOV2 domain in 20 mM Tris, 200 mM NaCl, pH 7 were collected on the 4C beam line at the Pohang Accelerator Laboratory (Pohang, South Korea) using a Rayonix SX165 detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.0734 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 24.98 mg/ml was measured at 24°C. 50 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 33.3,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": null,
  "porod_mw_error": null,
  "pddf_i0": null,
  "pddf_i0_error": null,
  "guinier_i0": 17.897,
  "guinier_i0_error": null,
  "pddf_rg": null,
  "pddf_rg_error": null,
  "guinier_rg": 2.26,
  "guinier_rg_error": 0.014,
  "pddf_dmax": 8.5,
  "pddf_dmax_error": null,
  "porod_volume": 51.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 1,
  "guinier_point_last": 210,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDV65_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2024-10-07T14:53:56.454816+02:00",
  "bragg_peak": []
}

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  "finished_at": "2026-08-11T14:40:40.914978+00:00",
  "source_last_modified": "2024-10-07T14:53:56.454816+02:00",
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