SASDV79

Mouse Central Communication Network 2 (CCN2) in complex with anti-CCN2 F(ab) antibody 37-45-MH1

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2025-10-29T10:20:00.805228+01:00
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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · Mouse Central Communication Network 2 (CCN2) in complex with anti-CCN2 F(ab) antibody 37-45-MH1

Concentration— – 5.0 Buffer / pH50 mM Tris pH 7.5, 200 mM NaCl / 7.5
Experimental temperature15.0 Facility / beamlineDiamond Light Source / B21
Wavelength0.09537 nmExposure3.0 s × 599

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
Cellular communication network factor 2
View sequence
>CCN2VPRGSGGASQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA
protein—P2926826–348monomerMolecule count 136.142 kDa
anti-CCN2 37-45-MH1 F(ab) antibody
View sequence
>MFABLIGHTCHAINANDHFABHEAVYCHAINAPLAEIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASRRATGIPDRFSGSGSGTDFTLTISRLEPEDFVVYFCQQYVSTPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECAPLAQVQLVQSGAEVKKPGASVKVSCKASGYTFTGYYMYWVRQAPGQGLEWMGWINPNSGGTNYSQKFQGRVTMTRDTSISTAYMELSRLRSDDTALYYCARGSKWNYPFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSGSTLVPR
protein———–—monomerMolecule count 148.799 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
12567[3]0.0045–0.34 1/AHas uncertainty columnMissing 0246sasbdb/entries/79/sasdv79/source/SASDV79.dat
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2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)11.9—Å
i0Guinier0.782483—arbitrary
i0P(r)0.792—arbitrary
mwExperimental67.7—kDa
mwPorod60.0—kDa
porod_volumePorod95.0—ų
rgGuinier3.260.233Å
rgP(r)3.39—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

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4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name50 mM Tris pH 7.5, 200 mM NaClBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2022-05-06Storage / measurement temperature4.0 / 15.0
Exposure time3.0Frames599
Wavelength0.09537Sample-to-detector distance3.688
SourceX-ray synchrotronDetectorEiger 4M
Facility / beamlineDiamond Light Source / B21 · Didcot, UK
q range0.045 – 3.4Sample volume / flow rate50.0 / 1.6

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

CategoryFileStatusSizeChecksumDownload and View
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sascifsasbdb/entries/79/sasdv79/source/SASDV79.sascifnot_available——DownloadView source fileSource site
summarysasbdb/entries/79/sasdv79/source/summary.jsondownloaded7845745153739e1c64b5dfe1be32b6ff8af929f2362d568329870af72d3a0ded975aDownloadView source fileSource site
curve:source record
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full_entry_zip:source record and ZIP contents (4 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:168 field values
Field pathRaw value
codeSASDV79
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleStructural basis for therapeutic antibody binding to CCN2 and its mechanisms on TGF-β family signaling
project.publicationnull
project.statusreleased
project.submitted_date2024-10-28
project.released_date2025-10-28
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intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDV79.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV79_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV79_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV79_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV79_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDV79.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger 4M
experiment.instrument.detector.resolution75.0
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameCellular communication network factor 2
experiment.sample.molecule[0].short_nameCCN2
experiment.sample.molecule[0].sequence>CCN2 VPRGSGGASQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA
experiment.sample.molecule[0].organismnull
experiment.sample.molecule[0].uniprot_codeP29268
experiment.sample.molecule[0].uniprot_range_first26
experiment.sample.molecule[0].uniprot_range_last348
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMLASVAGPISLALVLLALCTRPAMGQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCA KQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCK YQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAA YRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRP CEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTL PVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA
experiment.sample.molecule[0].mw36.142
experiment.sample.molecule[0].total_mw36.142
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionFull length and N-terminal fragment CCN2
experiment.sample.molecule[1].long_nameanti-CCN2 37-45-MH1 F(ab) antibody
experiment.sample.molecule[1].short_name37-45-MH1
experiment.sample.molecule[1].sequence>mFAb light chain and hFab heavy chain aplaeivltqspgtlslspgeratlscrasqsvsssylawyqqkpgqaprlliygasrratgipdrfsgsgsgtdftltisrlepedfvvyfcqqyvstpwtfgqgtkveikrtvaapsvfifppsdeqlksgtasvvcllnnfypreakvqwkvdnalqsgnsqesvteqdskdstyslsstltlskadyekhkvyacevthqglsspvtksfnrgec aplaqvqlvqsgaevkkpgasvkvsckasgytftgyymywvrqapgqglewmgwinpnsggtnysqkfqgrvtmtrdtsistaymelsrlrsddtalyycargskwnypfdywgqgtlvtvssastkgpsvfplapsskstsggtaalgclvkdyfpepvtvswnsgaltsgvhtfpavlqssglyslssvvtvpssslgtqtyicnvnhkpsntkvdkrvepksgstlvpr
experiment.sample.molecule[1].organismnull
experiment.sample.molecule[1].uniprot_codenull
experiment.sample.molecule[1].uniprot_range_firstnull
experiment.sample.molecule[1].uniprot_range_lastnull
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequence
experiment.sample.molecule[1].mw48.799
experiment.sample.molecule[1].total_mw48.799
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.buffer.name50 mM Tris pH 7.5, 200 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameMouse Central Communication Network 2 (CCN2) in complex with anti-CCN2 F(ab) antibody 37-45-MH1
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressWinnipeg, Canada
experiment.contributor[0].affiliation[0].full_nameUniversity of Manitoba
experiment.contributor[0].affiliation[0].webpagehttp://umanitoba.ca/
experiment.contributor[0].contributor_nameHaben
experiment.contributor[0].contributor_surnameGabir
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8775-6799
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2022-05-06
experiment.storage_temperature4.0
experiment.cell_temperature15.0
experiment.exposure_time3.0
experiment.number_of_frames599
experiment.wavelength0.09537
experiment.sample_detector_distance3.688
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume50.0
experiment.flow_rate1.6
experiment.s_min0.045
experiment.s_max3.4
experiment.total_exposure_timenull
experiment.seccolumnnull
fits[0].models[0].model_plotnull
fits[0].models[0].softwareDENSS
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modeldensity
fits[0].models[0].software_versionnull
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fits[0].models[0].bead_radiusnull
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fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1143
fits[0].fit_unit1/A
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fits[0].softwareDENSS
fits[0].chi_square_value0.0036
fits[0].p_value1.0
fits[0].fit_residual_plotSASDV79_fit1_fitresiduals_img.png
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fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw67.7
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw60.0
porod_mw_errornull
pddf_i00.792
pddf_i0_errornull
guinier_i00.782483
guinier_i0_errornull
pddf_rg3.39
pddf_rg_errornull
guinier_rg3.26
guinier_rg_error0.233
pddf_dmax11.9
pddf_dmax_errornull
porod_volume95.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first34
guinier_point_last202
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDV79_datloglog_img.png
symmetrynull
last_modified2025-10-29T10:20:00.805228+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDV79
statussuccess
started_at2026-08-11T16:08:05.529687+00:00
finished_at2026-08-11T16:08:14.964992+00:00
source_last_modified2025-10-29T10:20:00.805228+01:00
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View the complete summary.json source
{
  "code": "SASDV79",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Structural basis for therapeutic antibody binding to CCN2 and its mechanisms on TGF-β family signaling",
    "publication": null,
    "status": "released",
    "submitted_date": "2024-10-28",
    "released_date": "2025-10-28"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDV79.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDV79.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV79_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV79_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDV79_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDV79_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDV79.sascif",
  "experiment": {
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        "name": "Eiger 4M",
        "resolution": 75.0
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": null,
      "type_of_source": "X-ray synchrotron",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Cellular communication network factor 2",
          "short_name": "CCN2",
          "sequence": ">CCN2\r\nVPRGSGGASQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCKYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAAYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA",
          "organism": null,
          "uniprot_code": "P29268",
          "uniprot_range_first": 26,
          "uniprot_range_last": 348,
          "oligomerization": "monomer",
          "molecular_type": "protein",
          "uniprot_sequence": "MLASVAGPISLALVLLALCTRPAMGQDCSAQCQCAAEAAPHCPAGVSLVLDGCGCCRVCA\nKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCVFGGSVYRSGESFQSSCK\nYQCTCLDGAVGCVPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDRTAVGPALAA\nYRLEDTFGPDPTMMRANCLVQTTEWSACSKTCGMGISTRVTNDNTFCRLEKQSRLCMVRP\nCEADLEENIKKGKKCIRTPKIAKPVKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTL\nPVEFKCPDGEIMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA",
          "mw": 36.142,
          "total_mw": 36.142,
          "number_molecules": 1,
          "complex_state": false,
          "deuteration": null,
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View the complete manifest.json source
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