SASDV97

The 60bp dsDNA oligo GAGE6_2

Data type: SASBDB experimental data Status: Published Curve type: Single concentration Last updated: 2026-02-23T09:45:37.315609+01:00

5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCTTTTTGATGCT-3: The dsDNA oligo GAGE6_2 with the sequence

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · The 60bp dsDNA oligo GAGE6_2

Concentration— – 2.78 Buffer / pH150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT / 7.5
Experimental temperature25.0 Facility / beamlineAustralian Synchrotron / SAXS/WAXS
Wavelength0.10781 nmExposure1.0 s × 400

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
GAGE6_2
View sequence
GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCTTTTTGATGCT
DNAHomo sapiens——–—dimerMolecule count 218.57 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1684[3]0.006797766–0.6235348 1/AHas uncertainty columnMissing 0174sasbdb/entries/97/sasdv97/source/SASDV97.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)18.44—Å
i0Guinier0.0123382—1/cm
i0P(r)0.01292—1/cm
mwExperimental31.273—kDa
mwPorod102.0—kDa
porod_volumePorod72.0—ų
rgGuinier5.1971.9Å
rgP(r)5.51—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

This entry has no source fit record.

▶

4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTTBuffer concentration—
pH7.5Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-11-28Storage / measurement temperature25.0 / 25.0
Exposure time1.0Frames400
Wavelength0.10781Sample-to-detector distance2.39
SourceX-ray synchrotronDetectorPilatus3 S 2M
Facility / beamlineAustralian Synchrotron / SAXS/WAXS · Melbourne, Australia
q range0.068 – 6.235Sample volume / flow rate— / —

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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full_entry_zip:source record and ZIP contents (2 items)
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pddf:source record
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sascif:source record
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summary:source record
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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:134 field values
Field pathRaw value
codeSASDV97
statusPublished
type_of_curveSingle concentration
angular_unit1/A
project.titleProbing the statistics of sequence-dependent DNA conformations in solution using SAXS.
project.publication.titleProbing the statistics of sequence-dependent DNA conformations in solution using SAXS.
project.publication.author_listKoning HJ, Pullakhandam A, Whitten AE, Bond CS, Peyrard M
project.publication.journalActa Crystallogr D Struct Biol
project.publication.doi10.1107/S2059798325011556
project.publication.pmid41553140
project.publication.published_date2026 Feb 1
project.statusreleased
project.submitted_date2024-09-18
project.released_date2025-08-12
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experiment.instrument.detector.resolution172.0
experiment.instrument.nameAustralian Synchrotron
experiment.instrument.cityMelbourne
experiment.instrument.countryAustralia
experiment.instrument.beamline_nameSAXS/WAXS
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
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experiment.instrument.xray_energynull
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experiment.sample.molecule[0].long_nameGAGE6_2
experiment.sample.molecule[0].short_nameGAGE6_2
experiment.sample.molecule[0].sequenceGCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCTTTTTGATGCT
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codenull
experiment.sample.molecule[0].uniprot_range_firstnull
experiment.sample.molecule[0].uniprot_range_lastnull
experiment.sample.molecule[0].oligomerizationdimer
experiment.sample.molecule[0].molecular_typeDNA
experiment.sample.molecule[0].uniprot_sequence
experiment.sample.molecule[0].mw18.57
experiment.sample.molecule[0].total_mw37.14
experiment.sample.molecule[0].number_molecules2
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionDuplex DNA GAGE6_2: 5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCTTTTTGATGCT-3
experiment.sample.buffer.name150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameThe 60bp dsDNA oligo GAGE6_2
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameThe University of Western Australia
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameHeidar
experiment.contributor[0].contributor_surnameKoning
experiment.contributor[0].orcid
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-11-28
experiment.storage_temperature25.0
experiment.cell_temperature25.0
experiment.exposure_time1.0
experiment.number_of_frames400
experiment.wavelength0.10781
experiment.sample_detector_distance2.39
experiment.concentration_minnull
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experiment.seccolumnnull
fits[]
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
description5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCTTTTTGATGCT-3: The dsDNA oligo GAGE6_2 with the sequence
experiment_descriptionSynchrotron SAXS data from solutions of The 60bp dsDNA oligo GAGE6_2 in 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.10781 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One 50 µl solute concentration of 2.78 mg/ml was measured at 25°C. 400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. This data is part of the manuscript 'Probing the statistics of sequence-dependent DNA conformations in solution using SAXS'. 2sigma errors from the Australian Synchrotron's SAXS/WAXS beamline have been divided by 2 to be compatible with expectations from the ATSAS package.
tags[]
intensity_unit1/cm
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experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
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pddf_i0_errornull
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pddf_rg_errornull
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guinier_rg_error1.9
pddf_dmax18.44
pddf_dmax_errornull
porod_volume72.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first3
guinier_point_last21
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDV97_datloglog_img.png
symmetrynull
last_modified2026-02-23T09:45:37.315609+01:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDV97
statussuccess
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View the complete summary.json source
{
  "code": "SASDV97",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
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      "journal": "Acta Crystallogr D Struct Biol",
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      "pmid": "41553140",
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View the complete manifest.json source
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