2. Measurements Reported by SASBDB Reported Results
Metric
Method
Value
Uncertainty
Unit
dmax
P(r)
18.44
—
Å
i0
Guinier
0.0123382
—
1/cm
i0
P(r)
0.01292
—
1/cm
mw
Experimental
31.273
—
kDa
mw
Porod
102.0
—
kDa
porod_volume
Porod
72.0
—
ų
rg
Guinier
5.197
1.9
Å
rg
P(r)
5.51
—
Å
These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.
▼
3. Source Fits and Models Source Fits & Models
This entry has no source fit record.
▶
4. Source File Index Source Files
Category
Path
Download status
Size
curve
sasbdb/entries/97/sasdv97/source/SASDV97.dat
downloaded
27538
full_entry_zip
sasbdb/entries/97/sasdv97/source/SASDV97.zip
downloaded
15606
pddf
sasbdb/entries/97/sasdv97/source/SASDV97.out
downloaded
19296
sascif
sasbdb/entries/97/sasdv97/source/SASDV97.sascif
not_available
—
summary
sasbdb/entries/97/sasdv97/source/summary.json
downloaded
6356
▶
5. Experiment Description and Publication Experiment & Publication
Original Experiment Description
Synchrotron SAXS data from solutions of The 60bp dsDNA oligo GAGE6_2 in 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.10781 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One 50 µl solute concentration of 2.78 mg/ml was measured at 25°C. 400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. This data is part of the manuscript 'Probing the statistics of sequence-dependent DNA conformations in solution using SAXS'. 2sigma errors from the Australian Synchrotron's SAXS/WAXS beamline have been divided by 2 to be compatible with expectations from the ATSAS package.
Related Publication
Title
Probing the statistics of sequence-dependent DNA conformations in solution using SAXS.
Authors
Koning HJ, Pullakhandam A, Whitten AE, Bond CS, Peyrard M
DOI / PMID
10.1107/S2059798325011556 / 41553140
▼
6. Complete Source Record Complete Source Record
The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.
5-GCCTTCTGCAAAGAAGTCTTGCGCATCGCGCGTGAAGGCGCGCGCTAGCTTTTTGATGCT-3: The dsDNA oligo GAGE6_2 with the sequence
experiment_description
Synchrotron SAXS data from solutions of The 60bp dsDNA oligo GAGE6_2 in 150 mM KCl, 20 mM HEPES, 5% glycerol, 5 mM MgCl2, 1 mM DTT, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus3 S 2M detector at a sample-detector distance of 2.4 m and at a wavelength of λ = 0.10781 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One 50 µl solute concentration of 2.78 mg/ml was measured at 25°C. 400 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. This data is part of the manuscript 'Probing the statistics of sequence-dependent DNA conformations in solution using SAXS'. 2sigma errors from the Australian Synchrotron's SAXS/WAXS beamline have been divided by 2 to be compatible with expectations from the ATSAS package.