SASDVA4

β-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

Data type: SASBDB experimental data Status: Published Curve type: SEC-SAS Last updated: 2026-06-16T09:24:10.042679+02:00

Size-exclusion chromatography coupled small-angle X-ray scattering (SEC-SAXS) data of B75KN-CTD bound to LILRB3 were obtained using the SIBYLS beamline 12.3.1 at the Advanced Light Source, Lawrence Berkeley National Laboratory. The experiments were conducted with a sample-to-detector distance of 2.1 m and a wavelength. λ, of 0.1127 nm, yielding scattering vectors (s) ranging from 0.1 nm⁻¹ to 4 nm⁻¹. The scattering vector, s, is calculated using the equation s = 4πsinθ/λ, where 2θ represents the scattering angle. Size-exclusion chromatography (SEC) was performed using an Agilent 1260 series HPLC with a Shodex analytical column. A 60 μl sample at a 5 mg/ml protein concentration was injected at a flow rate of 0.65 ml/min onto a Shodex KW-803 column at 4°C. A total of 906 successive 2-second frames were captured. Subsequently, data normalization was conducted against the intensity of the transmitted beam, followed by radial averaging, and subtraction of the scattering from a solvent blank.

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1. Samples, Components and Experimental Conditions Sample & Experiment

Sample 1 · β-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3

Concentration— – 5.0 Buffer / pH20 mM HEPES, 300 mM NaCl / 7.4
Experimental temperature4.0 Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS)
Wavelength0.1127 nmExposure2.0 s × 906

Molecular Components

ComponentType / organismUniProt and ConstructOligomeric StateMolecular weight
IgA FC receptor
View sequence
KAGLDQEIQEHVKKETSSEENTQKVDEHYANSLQNLAQKSLEELDKATTNEQATQVKNQFLENAQKLKEIQPLIKETNVKLYKAMSESLEQVEKELKHNSEANLEDLVAKSKEIVREYEGKLNQSKNLPELKQLEEEAHSKLKQVVEDFRKKFKTSEQVTPKKRVKRDLAA
proteinStreptococcus agalactiaeP27951258–428monomerMolecule count 119.786 kDa
Leukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)
View sequence
GPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRLDKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGFYNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQALFPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQSLTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSRSHGGQYRCYGAHNLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSGHSGGSSLPPTGPPSTPGLGRYLEHHHHHH
proteinHomo sapiensA0A0G2JM4624–443monomerMolecule count 146.951 kDa

Experimental Curves

CurvePoints / columnsq rangeUncertainty qualityNegative-intensity pointsSource file
1906[3]0.011402–0.469864 1/AHas uncertainty columnMissing 010sasbdb/entries/a4/sasdva4/source/SASDVA4.dat
▼

2. Measurements Reported by SASBDB Reported Results

MetricMethodValueUncertaintyUnit
dmaxP(r)13.5—Å
i0Guinier90.0885—arbitrary
i0P(r)90.34—arbitrary
mwExperimental87.1—kDa
mwPorod126.0—kDa
porod_volumePorod202.0—ų
rgGuinier3.9120.027Å
rgP(r)3.97—Å

These values are reported by SASBDB; SAXSdb did not recalculate them from the experimental curve.

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3. Source Fits and Models Source Fits & Models

▶

4. Source File Index Source Files

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5. Experiment Description and Publication Experiment & Publication

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6. Complete Source Record Complete Source Record

The following content comes directly from the SASBDB entry. Missing values are shown as “—”; SAXSdb does not infer undeclared units.

Open the original SASBDB entry

Buffer and Sample Properties

Buffer name20 mM HEPES, 300 mM NaClBuffer concentration—
pH7.4Additives—
Buffer description—
Purity measurement method—Extinction coefficient—
Absorbance—Scattering contrast—
Specific volume / dry volume— / —Mixture / deuteration— / —

Acquisition Conditions and Instrument

Measurement date2023-11-17Storage / measurement temperature7.0 / 4.0
Exposure time2.0Frames906
Wavelength0.1127Sample-to-detector distance2.1
SourceX-ray synchrotronDetectorMAR 165 CCD
Facility / beamlineAdvanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q range0.114 – 4.699Sample volume / flow rate60.0 / 0.65

Original SASBDB Plots

Experimental I(q)
Experimental I(q)
Experimental I(q), log-log
Experimental I(q), log-log
Guinier plot
Guinier plot
Kratky plot
Kratky plot
P(r) plot
P(r) plot

Downloadable Files

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All source fields (unfiltered)

Every source field is expanded here, including empty values, list members and fields already shown above.

summary.json:173 field values
Field pathRaw value
codeSASDVA4
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleBacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
project.publication.titleBacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.
project.publication.author_listRumpret M, Lewis Marffy AL, Zhang Y, van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ
project.publication.journalNat Commun
project.publication.doi10.1038/s41467-026-74098-6
project.publication.pmid42277001
project.publication.published_date2026 Jun 11
project.statusreleased
project.submitted_date2024-07-11
project.released_date2026-06-16
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVA4.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVA4.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA4_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA4_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVA4_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA4_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVA4.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.nameMAR 165 CCD
experiment.instrument.detector.resolutionnull
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameIgA FC receptor
experiment.sample.molecule[0].short_nameB6C
experiment.sample.molecule[0].sequenceKAGLDQEIQEHVKKETSSEENTQKVDEHYANSLQNLAQKSLEELDKATTNEQATQVKNQFLENAQKLKEIQPLIKETNVKLYKAMSESLEQVEKELKHNSEANLEDLVAKSKEIVREYEGKLNQSKNLPELKQLEEEAHSKLKQVVEDFRKKFKTSEQVTPKKRVKRDLAA
experiment.sample.molecule[0].organismStreptococcus agalactiae
experiment.sample.molecule[0].uniprot_codeP27951
experiment.sample.molecule[0].uniprot_range_first258
experiment.sample.molecule[0].uniprot_range_last428
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMFKSNYERKMRYSIRKFSVGVASVAVASLFMGSVAHASELVKDDSVKTTEVAAKPYPSMA QTDQGNNSSSSELETTKMEIPTTDIKKAVEPVEKTAGETSATDTGKREKQLQQWKNNLKN DVDNTILSHEQKNEFKTKIDETNDSDALLELENQFNETNRLLHIKQHEEVEKDKKAKQQK TLKQSDTKVDLSNIDKELNHQKSQVEKMAEQKGITNEDKDSMLKKIEDIRKQAQQADKKE DAEVKVREELGKLFSSTKAGLDQEIQEHVKKETSSEENTQKVDEHYANSLQNLAQKSLEE LDKATTNEQATQVKNQFLENAQKLKEIQPLIKETNVKLYKAMSESLEQVEKELKHNSEAN LEDLVAKSKEIVREYEGKLNQSKNLPELKQLEEEAHSKLKQVVEDFRKKFKTSEQVTPKK RVKRDLAANENNQQKIELTVSPENITVYEGEDVKFTVTAKSDSKTTLDFSDLLTKYNPSV SDRISTNYKTNTDNHKIAEITIKNLKLNESQTVTLKAKDDSGNVVEKTFTITVQKKEEKQ VPKTPEQKDSKTEEKVPQEPKSNDKNQLQELIKSAQQELEKLEKAIKELMEQPEIPSNPE YGIQKSIWESQKEPIQEAITSFKKIIGDSSSKYYTEHYFNKYKSDFMNYQLHAQMEMLTR KVVQYMNKYPDNAEIKKIFESDMKRTKEDNYGSLENDALKGYFEKYFLTPFNKIKQIVDD LDKKVEQDQPAPIPENSEMDQAKEKAKIAVSKYMSKVLDGVHQHLQKKNNSKIVDLFKEL EAIKQQTIFDIDNAKTEVEIDNLVHDAFSKMNATVAKFQKGLETNTPETPDTPKIPELPQ APDTPQAPDTPHVPESPKAPEAPRVPESPKTPEAPHVPESPKAPEAPRVPESPKTPEAPH VPESPKTPEAPKIPEPPKTPDVPKLPDVPKLPDVPKLPDAPKLPDGLNKVGQAVFTSTDG NTKVTVVFDKPTDADKLHLKEVTTKELADKIAHKTGGGTVRVFDLSLSKGGKETHVNGER TVRLALGQTGSDVHVYHVKENGDLERIPSKVENGQVVFKTNHFSLFAIKTLSKDQNVTPP KQTKPSTQGSQVEIAESQTGKFQSKAANHKALATGNETVAKGNPTSTTEKKLPYTGVASN LVLEIMGLLGLIGTSFIAMKRRKS
experiment.sample.molecule[0].mw19.786
experiment.sample.molecule[0].total_mw19.786
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionβ-Antigen C Protein (βAC) N-terminal B6C domain
experiment.sample.molecule[1].long_nameLeukocyte immunoglobulin-like receptor subfamily B member 3 (P288R)
experiment.sample.molecule[1].short_nameLILRB3
experiment.sample.molecule[1].sequenceGPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRLDKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGFYNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQALFPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQSLTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSRSHGGQYRCYGAHNLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEGAAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSGHSGGSSLPPTGPPSTPGLGRYLEHHHHHH
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeA0A0G2JM46
experiment.sample.molecule[1].uniprot_range_first24
experiment.sample.molecule[1].uniprot_range_last443
experiment.sample.molecule[1].oligomerizationmonomer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMTPALTALLCLGLSLGPRTRVQAGPFPKPTLWAEPGSVISWGSPVTIWCQGSLEAQEYRL DKEGSPEPLDRNNPLEPKNKARFSIPSMTEHHAGRYRCHYYSSAGWSEPSDPLELVMTGF YNKPTLSALPSPVVASGGNMTLRCGSQKGYHHFVLMKEGEHQLPRTLDSQQLHSGGFQAL FPVGPVNPSHRWRFTCYYYYMNTPQVWSHPSDPLEILPSGVSRKPSLLTLQGPVLAPGQS LTLQCGSDVGYDRFVLYKEGERDFLQRPGQQPQAGLSQANFTLGPVSPSHGGQYRCYGAH NLSSEWSAPSDPLNILMAGQIYDTVSLSAQPGPTVASGENVTLLCQSWWQFDTFLLTKEG AAHPPLRLRSMYGAHKYQAEFPMSPVTSAHAGTYRCYGSYSSNPHLLSFPSEPLELMVSG HSGGSSLPPTGPPSTPGLGRYLEVLIGVSVAFVLLLFLLLFLLLRRQRHSKHRTSDQRKT DFQRPAGAAETEPKDRGLLRRSSPAADVQEENLYAAVKDTQSEDRVELDSQQSPHDEDPQ AVTYAPVKHSSPRREMASPPSSLSGEFLDTKDRQVEEDRQMDTEAAASEASQDVTYAQLH SLTLRRKATEPPPSQEGEPPAEPSIYATLAIH
experiment.sample.molecule[1].mw46.951
experiment.sample.molecule[1].total_mw46.951
experiment.sample.molecule[1].number_molecules1
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_descriptionThe protein construct used for SAXS contains an additional non-native polyhistidine tag at the C-terminus (HHHHHH).
experiment.sample.buffer.name20 mM HEPES, 300 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameβ-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameKansas State University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameYing
experiment.contributor[0].contributor_surnameZhang
experiment.contributor[0].orcidhttps://orcid.org/0009-0003-1603-7561
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-11-17
experiment.storage_temperature7.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames906
experiment.wavelength0.1127
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume60.0
experiment.flow_rate0.65
experiment.s_min0.114
experiment.s_max4.699
experiment.total_exposure_timenull
experiment.seccolumn40
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fits[0].models[0].commentnull
fits[0].models[0].user1086
fits[0].fit_unit1/A
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fits[0].softwareFoXS
fits[0].chi_square_value0.916447
fits[0].p_value0.0068
fits[0].fit_residual_plotSASDVA4_fit1_fitresiduals_img.png
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fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionSize-exclusion chromatography coupled small-angle X-ray scattering (SEC-SAXS) data of B75KN-CTD bound to LILRB3 were obtained using the SIBYLS beamline 12.3.1 at the Advanced Light Source, Lawrence Berkeley National Laboratory. The experiments were conducted with a sample-to-detector distance of 2.1 m and a wavelength. λ, of 0.1127 nm, yielding scattering vectors (s) ranging from 0.1 nm⁻¹ to 4 nm⁻¹. The scattering vector, s, is calculated using the equation s = 4πsinθ/λ, where 2θ represents the scattering angle. Size-exclusion chromatography (SEC) was performed using an Agilent 1260 series HPLC with a Shodex analytical column. A 60 μl sample at a 5 mg/ml protein concentration was injected at a flow rate of 0.65 ml/min onto a Shodex KW-803 column at 4°C. A total of 906 successive 2-second frames were captured. Subsequently, data normalization was conducted against the intensity of the transmitted beam, followed by radial averaging, and subtraction of the scattering from a solvent blank.
experiment_description-
tags[]
intensity_unitarbitrary
experimental_mw87.1
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mw126.0
porod_mw_errornull
pddf_i090.34
pddf_i0_errornull
guinier_i090.0885
guinier_i0_errornull
pddf_rg3.97
pddf_rg_errornull
guinier_rg3.912
guinier_rg_error0.027
pddf_dmax13.5
pddf_dmax_errornull
porod_volume202.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first10
guinier_point_last44
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVA4_datloglog_img.png
symmetrynull
last_modified2026-06-16T09:24:10.042679+02:00
bragg_peak[]
manifest.json:34 field values
Field pathRaw value
codeSASDVA4
statussuccess
started_at2026-08-11T16:30:38.210048+00:00
finished_at2026-08-11T16:30:46.888880+00:00
source_last_modified2026-06-16T09:24:10.042679+02:00
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View the complete summary.json source
{
  "code": "SASDVA4",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.",
    "publication": {
      "title": "Bacterial targeting of the neutrophil inhibitory receptor LILRB3 to evade antibody immunity.",
      "author_list": "Rumpret M, Lewis Marffy AL, Zhang Y, van Woudenbergh E, van der Lans SPA, Xu X, Fu Z, Zhao Y, Catton EA, Paré G, McGregor F, Spiller OB, Fernandes MJ, de Haas CJC, van Strijp JAG, van Sorge NM, Geisbrecht BV, McCarthy AJ",
      "journal": "Nat Commun",
      "doi": "10.1038/s41467-026-74098-6",
      "pmid": "42277001",
      "published_date": "2026 Jun 11"
    },
    "status": "released",
    "submitted_date": "2024-07-11",
    "released_date": "2026-06-16"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVA4.out",
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      },
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      "country": "USA",
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        {
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      "name": "β-Antigen C Protein B6C domain bound to Leukocyte Immunoglobulin-Like Receptor LILR-B3",
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      "contrast": null,
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        "affiliation": [
          {
            "short_name": null,
            "address": null,
            "full_name": "Kansas State University",
            "webpage": null
          }
        ],
        "contributor_name": "Ying",
        "contributor_surname": "Zhang",
        "orcid": "https://orcid.org/0009-0003-1603-7561"
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    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-11-17",
    "storage_temperature": 7.0,
    "cell_temperature": 4.0,
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    "wavelength": 0.1127,
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  "last_modified": "2026-06-16T09:24:10.042679+02:00",
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}
View the complete manifest.json source
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  "code": "SASDVA4",
  "status": "success",
  "started_at": "2026-08-11T16:30:38.210048+00:00",
  "finished_at": "2026-08-11T16:30:46.888880+00:00",
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