SASDVA6

Pentraxin-3

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-07-27T15:24:01.838202+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Pentraxin-3

浓度— – 6.0 缓冲液 / pH50 mM Tris-HCl, 150 mM NaCl / 7.4
实验温度20.0 设备 / 束线Diamond Light Source / B21
波长0.095 nm曝光1.0 s × 620

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Pentraxin-related protein PTX3
查看序列
ENSDDYDLMYVNLDNEIDNGLHPTEDPTPCACGQEHSEWDKLFIMLENSQMRERMLLQATDDVLRGELQRLREELGRLAESLARPCAPGAPAEARLTSALDELLQATRDAGRRLARMEGAEAQRPEEAGRALAAVLEELRQTRADLHAVQGWAARSWLPAGCETAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATDVLNKTILFSYGTKRNPYEIQLYLSYQSIVFVVGGEENKLVAEAMVSLGRWTHLCGTWNSEEGLTSLWVNGELAATTVEMATGHIVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIRGNIVGWGVTEIQPHGGAQYVS
proteinHomo sapiensP2602218–381octamer分子数 840.121 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11651[3]0.0035–0.3695563 1/A含误差列缺失 06sasbdb/entries/a6/sasdva6/source/SASDVA6.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)43.05—Å
i0Guinier0.487172—arbitrary
i0P(r)53.79153—arbitrary
mwExperimental323.873—kDa
porod_volumePorod658.0—ų
rgGuinier8.6490.397Å
rgP(r)9.73—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称50 mM Tris-HCl, 150 mM NaCl缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2018-09-28储存 / 测量温度4.0 / 20.0
曝光时间1.0帧数620
波长0.095样品-探测器距离2.7
光源X-ray synchrotron探测器Pilatus 2M
机构 / 束线Diamond Light Source / B21 · Didcot, UK
q 范围0.035 – 3.696样品体积 / 流速45.0 / 0.08

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/a6/sasdva6/source/SASDVA6.zipdownloaded9068971482127386cdf53873a6c9075484e1a787b5dff968e8767dc1fd86c6536739b6下载查看原文件源站
pddfsasbdb/entries/a6/sasdva6/source/SASDVA6.outdownloaded12984e3cb4300e275a355bb493f785d46da1883492b69662b1709e5cd816f428d9c59下载查看原文件源站
sascifsasbdb/entries/a6/sasdva6/source/SASDVA6.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/a6/sasdva6/source/summary.jsondownloaded814295197b57b75d21725115afd3f167fd181c9334c34764ca99dbf14bad24bea02d下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDVA6
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleThe structural organisation of pentraxin-3 and its interactions with heavy chains of inter-α-inhibitor regulate crosslinking of the hyaluronan matrix
project.publication.titleThe structural organisation of pentraxin-3 and its interactions with heavy chains of inter-α-inhibitor regulate crosslinking of the hyaluronan matrix
project.publication.author_listShah A, Zhang X, Snee M, Lockhart-Cairns M, Levy C, Jowitt T, Birchenough H, Dean L, Collins R, Dodd R, Roberts A, Enghild J, Mantovani A, Fontana J, Baldock C, Inforzato A, Richter R, Day A
project.publication.journalMatrix Biology
project.publication.doi10.1016/j.matbio.2025.01.002
project.publication.pmidnull
project.publication.published_date2025 Apr
project.statusreleased
project.submitted_date2024-08-19
project.released_date2025-07-27
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVA6.dat
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVA6.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA6_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA6_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVA6_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVA6_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVA6.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus 2M
experiment.instrument.detector.resolutionnull
experiment.instrument.nameDiamond Light Source
experiment.instrument.cityDidcot
experiment.instrument.countryUK
experiment.instrument.beamline_nameB21
experiment.instrument.beam_geometry1 x 5 mm
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_namePentraxin-related protein PTX3
experiment.sample.molecule[0].short_namePTX3
experiment.sample.molecule[0].sequenceENSDDYDLMYVNLDNEIDNGLHPTEDPTPCACGQEHSEWDKLFIMLENSQMRERMLLQATDDV LRGELQRLRE ELGRLAESLARPCAPGAPAEARLTSALDELLQATRDAGRRLARMEGAEAQRPEEAGRALA AVLEELRQTRADLHAVQGWAARSWLPAGCETAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATDVLN KTILFSYGTKRNPYEIQLYLSYQSIVFVVGGEENKLVAEAMVSLGRWTHLCGTWNSEEGLTSLWVNGELA ATTVEMATGHIVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIR GNIVGWGVTEIQPHGGAQYVS
experiment.sample.molecule[0].organismHomo sapiens
experiment.sample.molecule[0].uniprot_codeP26022
experiment.sample.molecule[0].uniprot_range_first18
experiment.sample.molecule[0].uniprot_range_last381
experiment.sample.molecule[0].oligomerizationoctamer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMHLLAILFCALWSAVLAENSDDYDLMYVNLDNEIDNGLHPTEDPTPCACGQEHSEWDKLF IMLENSQMRERMLLQATDDVLRGELQRLREELGRLAESLARPCAPGAPAEARLTSALDEL LQATRDAGRRLARMEGAEAQRPEEAGRALAAVLEELRQTRADLHAVQGWAARSWLPAGCE TAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATDVLNKTILFSYGTKRNPYEIQLYL SYQSIVFVVGGEENKLVAEAMVSLGRWTHLCGTWNSEEGLTSLWVNGELAATTVEMATGH IVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIR GNIVGWGVTEIQPHGGAQYVS
experiment.sample.molecule[0].mw40.121
experiment.sample.molecule[0].total_mw320.968
experiment.sample.molecule[0].number_molecules8
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionPTX3 was expressed in Chinese hamster ovary (CHO) cells and is therefore glycosylated. Signal sequence cleaved (amino acids 1-17 of UniProt P26022). D48 isoform.
experiment.sample.buffer.name50 mM Tris-HCl, 150 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.namePentraxin-3
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressManchester, UK
experiment.contributor[0].affiliation[0].full_nameUniversity of Manchester
experiment.contributor[0].affiliation[0].webpagehttp://www.manchester.ac.uk/
experiment.contributor[0].contributor_nameAnokhi
experiment.contributor[0].contributor_surnameShah
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-9818-5573
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2018-09-28
experiment.storage_temperature4.0
experiment.cell_temperature20.0
experiment.exposure_time1.0
experiment.number_of_frames620
experiment.wavelength0.095
experiment.sample_detector_distance2.7
experiment.concentration_minnull
experiment.concentration_max6.0
experiment.sample_volume45.0
experiment.flow_rate0.08
experiment.s_min0.035
experiment.s_max3.696
experiment.total_exposure_timenull
experiment.seccolumn59
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDVA6_fit1_model1_img.png
fits[0].models[0].softwareAllosMod
fits[0].models[0].pdb_link[]
fits[0].models[0].model_titlenull
fits[0].models[0].type_of_modelatomic
fits[0].models[0].software_versionnull
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fits[0].models[0].model_mw339.5
fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1107
fits[0].fit_unit1/A
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fits[0].softwareFoXS
fits[0].chi_square_value39.752855
fits[0].p_value0.0
fits[0].fit_residual_plotSASDVA6_fit1_fitresiduals_img.png
fits[0].fit_datahttps://www.sasbdb.org/media/fitting_files/SASDVA6_fit1.fit
fits[0].fit_lognull
fits[0].software_versionnull
fits[0].description
estimated_volume_methodnull
pddf_softwareScÅtter
pddf_software_versionIV
i0_calibration_standardnull
descriptionnull
experiment_descriptionSynchrotron SAXS data from solutions of Pentraxin-3 in 50 mM Tris-HCl, 150 mM NaCl, pH 7.4 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.095 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 6 mg/ml was injected at a 0.075 ml/min flow rate onto a Cytiva Superose 6 Increase 3.2/300 column at 20°C. 620 successive 1 second frames were collected throughout the entire SEC elution. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
tags[]
intensity_unitarbitrary
experimental_mw323.873
experimental_mw_errornull
guinier_i0_mwnull
guinier_i0_mw_errornull
porod_mwnull
porod_mw_errornull
pddf_i053.79153
pddf_i0_errornull
guinier_i00.487172
guinier_i0_errornull
pddf_rg9.73
pddf_rg_errornull
guinier_rg8.649
guinier_rg_error0.397
pddf_dmax43.05
pddf_dmax_errornull
porod_volume658.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first10
guinier_point_last49
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVA6_datloglog_img.png
symmetrynull
last_modified2025-07-27T15:24:01.838202+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVA6
statussuccess
started_at2026-08-11T15:05:21.655679+00:00
finished_at2026-08-11T15:05:30.223846+00:00
source_last_modified2025-07-27T15:24:01.838202+02:00
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files[4].zip_validationcentral_directory_readable
查看完整 summary.json 原文
{
  "code": "SASDVA6",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "The structural organisation of pentraxin-3 and its interactions with heavy chains of inter-α-inhibitor regulate crosslinking of the hyaluronan matrix",
    "publication": {
      "title": "The structural organisation of pentraxin-3 and its interactions with heavy chains of inter-α-inhibitor regulate crosslinking of the hyaluronan matrix",
      "author_list": "Shah A, Zhang X, Snee M, Lockhart-Cairns M, Levy C, Jowitt T, Birchenough H, Dean L, Collins R, Dodd R, Roberts A, Enghild J, Mantovani A, Fontana J, Baldock C, Inforzato A, Richter R, Day A",
      "journal": "Matrix Biology",
      "doi": "10.1016/j.matbio.2025.01.002",
      "pmid": null,
      "published_date": "2025 Apr"
    },
    "status": "released",
    "submitted_date": "2024-08-19",
    "released_date": "2025-07-27"
  },
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  "experiment": {
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        "name": "Pilatus 2M",
        "resolution": null
      },
      "name": "Diamond Light Source",
      "city": "Didcot",
      "country": "UK",
      "beamline_name": "B21",
      "beam_geometry": "1 x 5 mm",
      "type_of_source": "X-ray synchrotron",
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      "xray_energy": null,
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      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "Pentraxin-related protein PTX3",
          "short_name": "PTX3",
          "sequence": "ENSDDYDLMYVNLDNEIDNGLHPTEDPTPCACGQEHSEWDKLFIMLENSQMRERMLLQATDDV LRGELQRLRE ELGRLAESLARPCAPGAPAEARLTSALDELLQATRDAGRRLARMEGAEAQRPEEAGRALA AVLEELRQTRADLHAVQGWAARSWLPAGCETAILFPMRSKKIFGSVHPVRPMRLESFSACIWVKATDVLN KTILFSYGTKRNPYEIQLYLSYQSIVFVVGGEENKLVAEAMVSLGRWTHLCGTWNSEEGLTSLWVNGELA ATTVEMATGHIVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIR GNIVGWGVTEIQPHGGAQYVS",
          "organism": "Homo sapiens",
          "uniprot_code": "P26022",
          "uniprot_range_first": 18,
          "uniprot_range_last": 381,
          "oligomerization": "octamer",
          "molecular_type": "protein",
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          "molecule_source": "biological",
          "molecule_description": "PTX3 was expressed in Chinese hamster ovary (CHO) cells and is therefore glycosylated. Signal sequence cleaved (amino acids 1-17 of UniProt P26022). D48 isoform."
        }
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        "pka": null,
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      "name": "Pentraxin-3",
      "ext_coefficient": null,
      "contrast": null,
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      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Manchester, UK",
            "full_name": "University of Manchester",
            "webpage": "http://www.manchester.ac.uk/"
          }
        ],
        "contributor_name": "Anokhi",
        "contributor_surname": "Shah",
        "orcid": "https://orcid.org/0000-0001-9818-5573"
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2018-09-28",
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  "last_modified": "2025-07-27T15:24:01.838202+02:00",
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}
查看完整 manifest.json 原文
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