C-terminal truncation mutant of N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF

{
  "code": "SASDVB8",
  "status": "Published",
  "type_of_curve": "Single concentration",
  "angular_unit": "1/A",
  "project": {
    "title": "SidF, a dual substrate N5-acetyl-N5-hydroxy-L-ornithine transacetylase involved in Aspergillus fumigatus siderophore biosynthesis",
    "publication": {
      "title": "SidF, a dual substrate N5-acetyl-N5-hydroxy-L-ornithine transacetylase involved in Aspergillus fumigatus siderophore biosynthesis",
      "author_list": "Poonsiri T, Stransky J, Demitri N, Haas H, Cianci M, Benini S",
      "journal": "Journal of Structural Biology: X",
      "doi": "10.1016/j.yjsbx.2024.100119",
      "pmid": null,
      "published_date": "2025 Jun"
    },
    "status": "released",
    "submitted_date": "2024-10-21",
    "released_date": "2025-01-02"
  },
  "pddf_data": "https://www.sasbdb.org/media/p_of_R_files/SASDVB8.out",
  "intensities_data": "https://www.sasbdb.org/media/intensities_files/SASDVB8.dat",
  "intensities_log_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVB8_dat_img.png",
  "intensities_kratky_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVB8_kratky_img.png",
  "pddf_plot": "https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVB8_pofr_img.png",
  "intensities_guinier_plot": "https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVB8_guinier_img.png",
  "sascif_data": "https://www.sasbdb.org/media/sascif/sascif_files/SASDVB8.sascif",
  "experiment": {
    "instrument": {
      "detector": {
        "type": null,
        "name": "Eiger R 1M",
        "resolution": 0.75
      },
      "name": "Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure",
      "city": "Vestec",
      "country": "Czech Republic",
      "beamline_name": "Anton Paar SAXSpoint 2.0",
      "beam_geometry": null,
      "type_of_source": "X-ray in house",
      "point_source": null,
      "line_collimation": null,
      "sample_path_length": null,
      "line_collimation_slitlength": null,
      "line_collimation_integrationwidth": null,
      "xray_energy": null,
      "beam_profile_ah": null,
      "beam_profile_al": null
    },
    "sample": {
      "molecule": [
        {
          "long_name": "N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF (Δ444-462)",
          "short_name": "SidF C-trim",
          "sequence": "MGSSHHHHHHSSGENLYFQGHMATQSSTELPQINMTTAEPTSANKRTYPWVKLPHPYLTSYAIHVVSESTPRVLQLRLHDDQKGQALPEPLHSASLTYTDIAFDEAAQEIPDNDNSPWARSRRAPGTSFHWTGQEPPTLGQIWNVIHALFLTYPQHEIVRLDLNGSGKDIIREECLRTGLAVPFPSPRVPFGTENRPSETDTLILLRSAFWQGAGSPVGPRPIWAVDHGIHGLLRRSGSSYPPLAQNYEFSMKFPSERIYTRHPIRPQKPAPGSLVYSRYIPHLDQHFSLMVVDWQNEEHLQLFHKWQNDPRVAKGWNETGDLEHHRNYLRQLHEDKHVLCLFGRFDDFPFSYFEVYWAKEDHYGAHYDAGDYDRGRHSLVGESSVRGAYRVNAWWSSLIHYIFLDEPRTMCVVGEPKATNTTVLSYENAHGLTVQKYVDLGHKRSVHVYCSREKWFQLCPLFW",
          "organism": "Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)",
          "uniprot_code": "Q4WF55",
          "uniprot_range_first": 1,
          "uniprot_range_last": 443,
          "oligomerization": "dimer",
          "molecular_type": "protein",
          "uniprot_sequence": "MATQSSTELPQINMTTAEPTSANKRTYPWVKLPHPYLTSYAIHVVSESTPRVLQLRLHDD\nQKGQALPEPLHSASLTYTDIAFDEAAQEIPDNDNSPWARSRRAPGTSFHWTGQEPPTLGQ\nIWNVIHALFLTYPQHEIVRLDLNGSGKDIIREECLRTGLAVPFPSPRVPFGTENRPSETD\nTLILLRSAFWQGAGSPVGPRPIWAVDHGIHGLLRRSGSSYPPLAQNYEFSMKFPSERIYT\nRHPIRPQKPAPGSLVYSRYIPHLDQHFSLMVVDWQNEEHLQLFHKWQNDPRVAKGWNETG\nDLEHHRNYLRQLHEDKHVLCLFGRFDDFPFSYFEVYWAKEDHYGAHYDAGDYDRGRHSLV\nGESSVRGAYRVNAWWSSLIHYIFLDEPRTMCVVGEPKATNTTVLSYENAHGLTVQKYVDL\nGHKRSVHVYCSREKWFQLCPLFWDGRERPLESSDRMAWDAKL",
          "mw": 53.495,
          "total_mw": 106.99,
          "number_molecules": 2,
          "complex_state": false,
          "deuteration": null,
          "molecule_source": "biological",
          "molecule_description": "The protein construct used for SAXS contains a non-native polyhistidine tag, with additional non-native amino acids at the N-terminus (MGSSHHHHHHSSGENLYFQGH)."
        }
      ],
      "buffer": {
        "name": "50 mM Tris, 200 mM NaCl",
        "concentration_unit": null,
        "comment": null,
        "additive": null,
        "concentration": null,
        "pka": null,
        "ph": 8.0,
        "deuteration": null
      },
      "purity_method": null,
      "name": "C-terminal truncation mutant of N(5)-hydroxyornithine:cis-anhydromevalonyl coenzyme A-N(5)-transacylase sidF",
      "ext_coefficient": null,
      "contrast": null,
      "specific_vol": null,
      "dry_vol": null,
      "absorbption": null,
      "deuteration": null,
      "mixture": null
    },
    "contributor": [
      {
        "affiliation": [
          {
            "short_name": null,
            "address": "Thailand",
            "full_name": "Mahidol University",
            "webpage": "https://mahidol.ac.th/"
          }
        ],
        "contributor_name": "Thanalai",
        "contributor_surname": "Poonsiri",
        "orcid": ""
      }
    ],
    "concentration_method": null,
    "concentration_unit": null,
    "date": "2023-01-18",
    "storage_temperature": 20.0,
    "cell_temperature": 20.0,
    "exposure_time": 15.0,
    "number_of_frames": 60,
    "wavelength": 0.134,
    "sample_detector_distance": 0.571,
    "concentration_min": 5.0,
    "concentration_max": 5.0,
    "sample_volume": 400.0,
    "flow_rate": 0.05,
    "s_min": 0.124,
    "s_max": 3.027,
    "total_exposure_time": null,
    "seccolumn": null
  },
  "fits": [
    {
      "models": [
        {
          "model_plot": "https://www.sasbdb.org/media/pdb_file/images/SASDVB8_fit1_model1_img.png",
          "software": "CORAL",
          "pdb_link": [],
          "model_title": null,
          "type_of_model": "mix",
          "software_version": "5",
          "model_data": "https://www.sasbdb.org/media/pdb_file/SASDVB8_fit1_model1.pdb",
          "model_mw": 101.3,
          "bead_radius": 1.9,
          "log": null,
          "symmetry": null,
          "comment": null,
          "user": 1131
        }
      ],
      "fit_unit": "1/A",
      "fit_plot": "https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDVB8_fit1_fixed_fit_img.png",
      "software": "CORAL",
      "chi_square_value": 1.87,
      "p_value": 0.0,
      "fit_residual_plot": "SASDVB8_fit1_fitresiduals_img.png",
      "fit_data": "https://www.sasbdb.org/media/fitting_files/SASDVB8_fit1.fit",
      "fit_log": "https://www.sasbdb.org/media/fitting_files/log_files/BG_dom03.log",
      "software_version": "5",
      "description": ""
    }
  ],
  "estimated_volume_method": null,
  "pddf_software": "ATSAS GNOM",
  "pddf_software_version": "5.0",
  "i0_calibration_standard": null,
  "description": null,
  "experiment_description": "SAXS data from solutions of C-terminal truncated sidF in 50 mM Tris, 200 mM NaCl, pH 8 were collected using an Anton Paar SAXSpoint 2.0 instrument at the Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure (Vestec, Czech Republic) equipped with an Eiger R 1M detector at a sample-detector distance of 0.6 m and at a wavelength of λ = 0.134 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A sample concentration at 5 mg/ml was measured at 20°C. 60 successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.",
  "tags": [],
  "intensity_unit": "arbitrary",
  "experimental_mw": 109.1,
  "experimental_mw_error": null,
  "guinier_i0_mw": null,
  "guinier_i0_mw_error": null,
  "porod_mw": 118.0,
  "porod_mw_error": null,
  "pddf_i0": 0.2812,
  "pddf_i0_error": null,
  "guinier_i0": 0.276504,
  "guinier_i0_error": null,
  "pddf_rg": 3.68,
  "pddf_rg_error": null,
  "guinier_rg": 3.452,
  "guinier_rg_error": 0.208,
  "pddf_dmax": 15.0,
  "pddf_dmax_error": null,
  "porod_volume": 189.0,
  "porod_volume_error": null,
  "estimated_volume": null,
  "estimated_volume_error": null,
  "guinier_point_first": 5,
  "guinier_point_last": 43,
  "pddf_point_first": null,
  "pddf_point_last": null,
  "i0_calibration_standard_data": null,
  "intensities_log_log_plot": "SASDVB8_datloglog_img.png",
  "symmetry": null,
  "last_modified": "2025-01-02T12:10:48.459172+01:00",
  "bragg_peak": []
}

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  "finished_at": "2026-08-11T16:33:22.384744+00:00",
  "source_last_modified": "2025-01-02T12:10:48.459172+01:00",
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