SASDVC7

Tetrameric L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2025-06-12T16:25:58.703872+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Tetrameric L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli

浓度— – 1.03 缓冲液 / pH20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP, / 7.5
实验温度20.0 设备 / 束线Advanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID
波长0.1033 nm曝光0.5 s × 3600

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
L-threonine dehydratase biosynthetic IlvA
查看序列
MGSSHHHHHHSSGLVPRGSHADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG
proteinEscherichia coli (strain K12)P049682–514tetramer分子数 458.227 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
11698[3]0.00292059962–0.420131363 1/A含误差列缺失 074sasbdb/entries/c7/sasdvc7/source/SASDVC7.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)15.6—Å
i0Guinier0.0486689—arbitrary
i0P(r)0.04888—arbitrary
mwExperimental239.6—kDa
mwGuinier I(0)239.6—kDa
mwPorod236.0—kDa
porod_volumePorod284.0—ų
rgGuinier4.4760.06Å
rgP(r)4.56—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,缓冲液浓度—
pH7.5添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2022-10-14储存 / 测量温度20.0 / 20.0
曝光时间0.5帧数3600
波长0.1033样品-探测器距离3.663
光源X-ray synchrotron探测器Eiger2 XE 9M
机构 / 束线Advanced Photon Source (APS), Argonne National Laboratory / BioCAT 18ID · Lemont, IL, USA
q 范围0.029 – 4.201样品体积 / 流速300.0 / 0.6

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
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full_entry_zipsasbdb/entries/c7/sasdvc7/source/SASDVC7.zipdownloaded406659b9232d88c60e37d237ce18ad41139e0d77504cdd341f831d94e1317842dba5ea下载查看原文件源站
pddfsasbdb/entries/c7/sasdvc7/source/SASDVC7.outdownloaded14672952d55de78e0f8faf21c53b4a1fcd5456f2c1464fdf334825535fac61b118ed42下载查看原文件源站
sascifsasbdb/entries/c7/sasdvc7/source/SASDVC7.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/c7/sasdvc7/source/summary.jsondownloaded7423a0e678b2c3133dcc0960cda5da2962a7023897c57e174bd52b219ef9b9a5f1d5下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(4 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:156 个字段值
字段路径原始值
codeSASDVC7
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleIsoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA).
project.publication.titleIsoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA).
project.publication.author_listYun MK, Subramanian C, Miller K, Jackson P, Radka CD, Rock CO
project.publication.journalBiochemistry
project.publication.doi10.1021/acs.biochem.5c00168
project.publication.pmid40494512
project.publication.published_date2025 Jun 10
project.statusreleased
project.submitted_date2024-09-16
project.released_date2025-06-12
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experiment.instrument.detector.typenull
experiment.instrument.detector.nameEiger2 XE 9M
experiment.instrument.detector.resolution72.0
experiment.instrument.nameAdvanced Photon Source (APS), Argonne National Laboratory
experiment.instrument.cityLemont, IL
experiment.instrument.countryUSA
experiment.instrument.beamline_nameBioCAT 18ID
experiment.instrument.beam_geometrynull
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameL-threonine dehydratase biosynthetic IlvA
experiment.sample.molecule[0].short_nameIlvA
experiment.sample.molecule[0].sequenceMGSSHHHHHHSSGLVPRGSHADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG
experiment.sample.molecule[0].organismEscherichia coli (strain K12)
experiment.sample.molecule[0].uniprot_codeP04968
experiment.sample.molecule[0].uniprot_range_first2
experiment.sample.molecule[0].uniprot_range_last514
experiment.sample.molecule[0].oligomerizationtetramer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHS FKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDA VRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHL DRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAE GVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYI ALHNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLG GRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVR YMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFE LGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG
experiment.sample.molecule[0].mw58.227
experiment.sample.molecule[0].total_mw232.908
experiment.sample.molecule[0].number_molecules4
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_description
experiment.sample.buffer.name20 mM KPO4, 200 mM NaCl, 0.1 mM TCEP,
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.5
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameTetrameric L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameSt. Jude Children's Research Hospital
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameMi-Kyung
experiment.contributor[0].contributor_surnameYun
experiment.contributor[0].orcidhttps://orcid.org/0000-0003-1213-5714
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2022-10-14
experiment.storage_temperature20.0
experiment.cell_temperature20.0
experiment.exposure_time0.5
experiment.number_of_frames3600
experiment.wavelength0.1033
experiment.sample_detector_distance3.663
experiment.concentration_minnull
experiment.concentration_max1.03
experiment.sample_volume300.0
experiment.flow_rate0.6
experiment.s_min0.029
experiment.s_max4.201
experiment.total_exposure_timenull
experiment.seccolumn47
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDVC7_fit1_model1_img.png
fits[0].models[0].softwareChimera
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fits[0].models[0].commentnull
fits[0].models[0].user1105
fits[0].fit_unit1/A
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fits[0].softwareFoXS
fits[0].chi_square_value1.39974
fits[0].p_value0.0
fits[0].fit_residual_plotSASDVC7_fit1_fitresiduals_img.png
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fits[0].description
estimated_volume_methodnull
pddf_softwareATSAS GNOM
pddf_software_version5.0
i0_calibration_standardnull
descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw239.6
experimental_mw_errornull
guinier_i0_mw239.6
guinier_i0_mw_errornull
porod_mw236.0
porod_mw_errornull
pddf_i00.04888
pddf_i0_errornull
guinier_i00.0486689
guinier_i0_errornull
pddf_rg4.56
pddf_rg_errornull
guinier_rg4.476
guinier_rg_error0.06
pddf_dmax15.6
pddf_dmax_errornull
porod_volume284.0
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first37
guinier_point_last206
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVC7_datloglog_img.png
symmetrynull
last_modified2025-06-12T16:25:58.703872+02:00
bragg_peak[]
manifest.json:34 个字段值
字段路径原始值
codeSASDVC7
statussuccess
started_at2026-08-11T15:27:04.961192+00:00
finished_at2026-08-11T15:27:13.891218+00:00
source_last_modified2025-06-12T16:25:58.703872+02:00
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查看完整 summary.json 原文
{
  "code": "SASDVC7",
  "status": "Published",
  "type_of_curve": "SEC-SAS",
  "angular_unit": "1/A",
  "project": {
    "title": "Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA).",
    "publication": {
      "title": "Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA).",
      "author_list": "Yun MK, Subramanian C, Miller K, Jackson P, Radka CD, Rock CO",
      "journal": "Biochemistry",
      "doi": "10.1021/acs.biochem.5c00168",
      "pmid": "40494512",
      "published_date": "2025 Jun 10"
    },
    "status": "released",
    "submitted_date": "2024-09-16",
    "released_date": "2025-06-12"
  },
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        "resolution": 72.0
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      "city": "Lemont, IL",
      "country": "USA",
      "beamline_name": "BioCAT 18ID",
      "beam_geometry": null,
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    },
    "sample": {
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          "long_name": "L-threonine dehydratase biosynthetic IlvA",
          "short_name": "IlvA",
          "sequence": "MGSSHHHHHHSSGLVPRGSHADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRLDNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLGVKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHPMVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSACLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKDLFEDVRAVAEPSGALALAGMKKYIALHNIRGERLAHILSGANVNFHGLRYVSERCELGEQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEERKEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRFLNTLGTYWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAFRFFLAG",
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}
查看完整 manifest.json 原文
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