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SASDVF2

Staphylococcal extracellular adherence protein (Eap) in unbound form

数据类型：SASBDB 实验数据 状态：Published 曲线类型：SEC-SAS 最后更新：2024-10-07T14:58:22.951844+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Staphylococcal extracellular adherence protein (Eap) in unbound form

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 140 mM NaCl / 7.4
实验温度4.0 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.1127 nm曝光2.0 s × 660

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Protein map
查看序列
AAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
proteinStaphylococcus aureus (strain Mu50 / ATCC 700699)Q99QS132–478monomer分子数 150.322 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1912[3]0.011402–0.472902 1/A含误差列缺失 026sasbdb/entries/f2/sasdvf2/source/SASDVF2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
i0Guinier53.1382—arbitrary
mwExperimental47.1—kDa
mwGuinier I(0)54.540.5kDa
mwPorod47.0—kDa
porod_volumePorod63.7—ų
rgGuinier4.030.514Å

这些数值是 SASBDB 来源记录，不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”；未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 140 mM NaCl缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-06-03储存 / 测量温度4.0 / 4.0
曝光时间2.0帧数660
波长0.1127样品-探测器距离2.1
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q 范围0.114 – 4.729样品体积 / 流速60.0 / 0.65

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图

可下载文件

类别文件状态大小校验值下载与查看
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pddfsasbdb/entries/f2/sasdvf2/source/SASDVF2.outnot_listed——下载查看原文件
sascifsasbdb/entries/f2/sasdvf2/source/SASDVF2.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/f2/sasdvf2/source/summary.jsondownloaded10709632c776e9b2ca0d49e6668c1b9022a448956f544a4d74e10dda7f6f4bc01cd44下载查看原文件源站
curve：来源记录
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full_entry_zip：来源记录与 ZIP 内部目录（10 项）
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pddf：来源记录
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sascif：来源记录
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summary：来源记录
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全部来源字段（无筛选）

这里自动展开来源记录中的每一个字段，包括空值、列表成员和页面上方已展示过的字段。

summary.json：241 个字段值
字段路径原始值
codeSASDVF2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.author_listMishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
project.publication.journalJ Biol Chem
project.publication.doi10.1016/j.jbc.2024.107627
project.publication.pmid39098536
project.publication.published_date2024 Aug 3
project.statusreleased
project.submitted_date2024-06-17
project.released_date2024-07-22
pddf_datanull
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intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVF2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVF2_kratky_img.png
pddf_plotnull
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVF2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVF2.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProtein map
experiment.sample.molecule[0].short_nameEap
experiment.sample.molecule[0].sequenceAAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[0].organismStaphylococcus aureus (strain Mu50 / ATCC 700699)
experiment.sample.molecule[0].uniprot_codeQ99QS1
experiment.sample.molecule[0].uniprot_range_first32
experiment.sample.molecule[0].uniprot_range_last478
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGT SQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVI DLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFN KNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANL INSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLT SQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEI TVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISD LDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[0].mw50.322
experiment.sample.molecule[0].total_mw50.322
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionExtracellular adherence protein
experiment.sample.buffer.name20 mM HEPES, 140 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameStaphylococcal extracellular adherence protein (Eap) in unbound form
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameKansas State University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameNitin
experiment.contributor[0].contributor_surnameMishra
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8931-7351
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-06-03
experiment.storage_temperature4.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames660
experiment.wavelength0.1127
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume60.0
experiment.flow_rate0.65
experiment.s_min0.114
experiment.s_max4.729
experiment.total_exposure_timenull
experiment.seccolumn18
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fits[0].p_value0.0535
fits[0].fit_residual_plotSASDVF2_fit1_fitfoxsresiduals_img.png
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fits[1].chi_square_value1.107999
fits[1].p_value0.8343
fits[1].fit_residual_plotSASDVF2_fit2_fitfoxsresiduals_img.png
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fits[2].chi_square_value1.094022
fits[2].p_value0.8343
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descriptionnull
experiment_descriptionnull
tags[]
intensity_unitarbitrary
experimental_mw47.1
experimental_mw_errornull
guinier_i0_mw54.54
guinier_i0_mw_error0.5
porod_mw47.0
porod_mw_errornull
pddf_i0null
pddf_i0_errornull
guinier_i053.1382
guinier_i0_errornull
pddf_rgnull
pddf_rg_errornull
guinier_rg4.03
guinier_rg_error0.514
pddf_dmaxnull
pddf_dmax_errornull
porod_volume63.7
porod_volume_errornull
estimated_volumenull
estimated_volume_errornull
guinier_point_first15
guinier_point_last44
pddf_point_firstnull
pddf_point_lastnull
i0_calibration_standard_datanull
intensities_log_log_plotSASDVF2_datloglog_img.png
symmetrynull
last_modified2024-10-07T14:58:22.951844+02:00
bragg_peak[]
manifest.json：31 个字段值
字段路径原始值
codeSASDVF2
statussuccess
started_at2026-08-11T16:39:25.706723+00:00
finished_at2026-08-11T16:39:32.478874+00:00
source_last_modified2024-10-07T14:58:22.951844+02:00
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查看完整 summary.json 原文
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    "title": "S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.",
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      "title": "S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.",
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查看完整 manifest.json 原文
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