SASDVG2

Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G

数据类型:SASBDB 实验数据 状态:Published 曲线类型:SEC-SAS 最后更新:2024-10-07T14:58:23.076062+02:00
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1. 样品、组分与实验条件 Sample & Experiment

样品 1 · Staphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G

浓度— – 5.0 缓冲液 / pH20 mM HEPES, 140 mM NaCl / 7.4
实验温度4.0 设备 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS)
波长0.1127 nm曝光2.0 s × 660

分子组分

组分类型 / 物种UniProt 与构建体寡聚状态分子量
Protein map
查看序列
AAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
proteinStaphylococcus aureus (strain Mu50 / ATCC 700699)Q99QS132–478monomer分子数 150.322 kDa
Cathepsin G
查看序列
IIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVLTAAHCWGSNINVTLGAHNIQRRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRRNRNVNPVALPRAQEGLRPGTLCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAFKGDSGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRTTMR
proteinHomo sapiensP0831122–245tetramer分子数 425.354 kDa

实验曲线

曲线点数 / 列q 范围误差质量负强度点来源文件
1867[3]0.011402–0.450121 1/A含误差列缺失 033sasbdb/entries/g2/sasdvg2/source/SASDVG2.dat
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2. SASBDB 报告的指标 Reported Results

指标方法数值误差单位
dmaxP(r)20.2—Å
i0Guinier145.606—arbitrary
i0P(r)153.2—arbitrary
mwExperimental117.3—kDa
mwPorod100.0—kDa
porod_volumePorod166.0—ų
rgGuinier5.0320.499Å
rgP(r)5.5—Å

这些数值是 SASBDB 来源记录,不是 SAXSdb 对实验曲线重新计算的结果。

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3. 来源拟合与模型 Source Fits & Models

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4. 来源文件索引 Source Files

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5. 实验说明与论文 Experiment & Publication

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6. 完整来源记录 Complete Source Record

下列内容直接来自 SASBDB 条目。字段没有值时显示“—”;未声明的单位不会由 SAXSdb 猜测。

打开 SASBDB 原始条目

缓冲液与样品属性

缓冲液名称20 mM HEPES, 140 mM NaCl缓冲液浓度—
pH7.4添加剂—
缓冲液说明—
纯度测定方法—消光系数—
吸收值—散射对比度—
比体积 / 干体积— / —混合物 / 氘代— / —

采集条件与仪器

测量日期2023-06-03储存 / 测量温度4.0 / 4.0
曝光时间2.0帧数660
波长0.1127样品-探测器距离2.1
光源X-ray synchrotron探测器Pilatus3 X 2M
机构 / 束线Advanced Light Source (ALS) / 12.3.1 (SIBYLS) · Berkeley, CA, USA
q 范围0.114 – 4.501样品体积 / 流速60.0 / 0.65

SASBDB 原始图

实验 I(q)
实验 I(q)
实验 I(q) log-log
实验 I(q) log-log
Guinier 图
Guinier 图
Kratky 图
Kratky 图
P(r) 图
P(r) 图

可下载文件

类别文件状态大小校验值下载与查看
curvesasbdb/entries/g2/sasdvg2/source/SASDVG2.datdownloaded1426599196c15ea8f7802fc92d656497490b87a691e92aa37e2cf8165d1c7f3337da94下载查看原文件源站
full_entry_zipsasbdb/entries/g2/sasdvg2/source/SASDVG2.zipdownloaded1346020e067dda796dd9d762cce03d71d6885057ce69637f34e1a957996de44be033cb1下载查看原文件源站
pddfsasbdb/entries/g2/sasdvg2/source/SASDVG2.outdownloaded82177b32fcf13dd328deb3a8da319517a14639f331cf1534e7444a10180a8cce15b22下载查看原文件源站
sascifsasbdb/entries/g2/sasdvg2/source/SASDVG2.sascifnot_available——下载查看原文件源站
summarysasbdb/entries/g2/sasdvg2/source/summary.jsondownloaded11935975975a680a95e8a51747695bb1f7d8bb6ff94d16cb2019480a7037d9042bf64下载查看原文件源站
curve:来源记录
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full_entry_zip:来源记录与 ZIP 内部目录(11 项)
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pddf:来源记录
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sascif:来源记录
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summary:来源记录
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全部来源字段(无筛选)

这里自动展开来源记录中的每一个字段,包括空值、列表成员和页面上方已展示过的字段。

summary.json:258 个字段值
字段路径原始值
codeSASDVG2
statusPublished
type_of_curveSEC-SAS
angular_unit1/A
project.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.titleS. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.
project.publication.author_listMishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV
project.publication.journalJ Biol Chem
project.publication.doi10.1016/j.jbc.2024.107627
project.publication.pmid39098536
project.publication.published_date2024 Aug 3
project.statusreleased
project.submitted_date2024-06-17
project.released_date2024-07-22
pddf_datahttps://www.sasbdb.org/media/p_of_R_files/SASDVG2.out
intensities_datahttps://www.sasbdb.org/media/intensities_files/SASDVG2.dat
intensities_log_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVG2_dat_img.png
intensities_kratky_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVG2_kratky_img.png
pddf_plothttps://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDVG2_pofr_img.png
intensities_guinier_plothttps://www.sasbdb.org/media/intensities_files/scattering_plots/SASDVG2_guinier_img.png
sascif_datahttps://www.sasbdb.org/media/sascif/sascif_files/SASDVG2.sascif
experiment.instrument.detector.typenull
experiment.instrument.detector.namePilatus3 X 2M
experiment.instrument.detector.resolution172.0
experiment.instrument.nameAdvanced Light Source (ALS)
experiment.instrument.cityBerkeley, CA
experiment.instrument.countryUSA
experiment.instrument.beamline_name12.3.1 (SIBYLS)
experiment.instrument.beam_geometry
experiment.instrument.type_of_sourceX-ray synchrotron
experiment.instrument.point_sourcenull
experiment.instrument.line_collimationnull
experiment.instrument.sample_path_lengthnull
experiment.instrument.line_collimation_slitlengthnull
experiment.instrument.line_collimation_integrationwidthnull
experiment.instrument.xray_energynull
experiment.instrument.beam_profile_ahnull
experiment.instrument.beam_profile_alnull
experiment.sample.molecule[0].long_nameProtein map
experiment.sample.molecule[0].short_nameEap
experiment.sample.molecule[0].sequenceAAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[0].organismStaphylococcus aureus (strain Mu50 / ATCC 700699)
experiment.sample.molecule[0].uniprot_codeQ99QS1
experiment.sample.molecule[0].uniprot_range_first32
experiment.sample.molecule[0].uniprot_range_last478
experiment.sample.molecule[0].oligomerizationmonomer
experiment.sample.molecule[0].molecular_typeprotein
experiment.sample.molecule[0].uniprot_sequenceMKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGT SQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVI DLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFN KNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANL INSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLT SQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEI TVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISD LDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK
experiment.sample.molecule[0].mw50.322
experiment.sample.molecule[0].total_mw50.322
experiment.sample.molecule[0].number_molecules1
experiment.sample.molecule[0].complex_stateFalse
experiment.sample.molecule[0].deuterationnull
experiment.sample.molecule[0].molecule_sourcebiological
experiment.sample.molecule[0].molecule_descriptionExtracellular adherence protein
experiment.sample.molecule[1].long_nameCathepsin G
experiment.sample.molecule[1].short_nameCG
experiment.sample.molecule[1].sequenceIIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVLTAAHCWGSNINVTLGAHNIQRRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRRNRNVNPVALPRAQEGLRPGTLCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYDPRRQICVGDRRERKAAFKGDSGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRTTMR
experiment.sample.molecule[1].organismHomo sapiens
experiment.sample.molecule[1].uniprot_codeP08311
experiment.sample.molecule[1].uniprot_range_first22
experiment.sample.molecule[1].uniprot_range_last245
experiment.sample.molecule[1].oligomerizationtetramer
experiment.sample.molecule[1].molecular_typeprotein
experiment.sample.molecule[1].uniprot_sequenceMQPLLLLLAFLLPTGAEAGEIIGGRESRPHSRPYMAYLQIQSPAGQSRCGGFLVREDFVL TAAHCWGSNINVTLGAHNIQRRENTQQHITARRAIRHPQYNQRTIQNDIMLLQLSRRVRR NRNVNPVALPRAQEGLRPGTLCTVAGWGRVSMRRGTDTLREVQLRVQRDRQCLRIFGSYD PRRQICVGDRRERKAAFKGDSGGPLLCNNVAHGIVSYGKSSGVPPEVFTRVSSFLPWIRT TMRSFKLLDQMETPL
experiment.sample.molecule[1].mw25.354
experiment.sample.molecule[1].total_mw101.416
experiment.sample.molecule[1].number_molecules4
experiment.sample.molecule[1].complex_stateFalse
experiment.sample.molecule[1].deuterationnull
experiment.sample.molecule[1].molecule_sourcebiological
experiment.sample.molecule[1].molecule_description
experiment.sample.buffer.name20 mM HEPES, 140 mM NaCl
experiment.sample.buffer.concentration_unitnull
experiment.sample.buffer.commentnull
experiment.sample.buffer.additivenull
experiment.sample.buffer.concentrationnull
experiment.sample.buffer.pkanull
experiment.sample.buffer.ph7.4
experiment.sample.buffer.deuterationnull
experiment.sample.purity_methodnull
experiment.sample.nameStaphylococcal extracellular adherence protein (Eap) bound to human cathepsin-G
experiment.sample.ext_coefficientnull
experiment.sample.contrastnull
experiment.sample.specific_volnull
experiment.sample.dry_volnull
experiment.sample.absorbptionnull
experiment.sample.deuterationnull
experiment.sample.mixturenull
experiment.contributor[0].affiliation[0].short_namenull
experiment.contributor[0].affiliation[0].addressnull
experiment.contributor[0].affiliation[0].full_nameKansas State University
experiment.contributor[0].affiliation[0].webpagenull
experiment.contributor[0].contributor_nameNitin
experiment.contributor[0].contributor_surnameMishra
experiment.contributor[0].orcidhttps://orcid.org/0000-0001-8931-7351
experiment.concentration_methodnull
experiment.concentration_unitnull
experiment.date2023-06-03
experiment.storage_temperature4.0
experiment.cell_temperature4.0
experiment.exposure_time2.0
experiment.number_of_frames660
experiment.wavelength0.1127
experiment.sample_detector_distance2.1
experiment.concentration_minnull
experiment.concentration_max5.0
experiment.sample_volume60.0
experiment.flow_rate0.65
experiment.s_min0.114
experiment.s_max4.501
experiment.total_exposure_timenull
experiment.seccolumn40
fits[0].models[0].model_plothttps://www.sasbdb.org/media/pdb_file/images/SASDVG2_fit1_model1_img.png
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fits[0].models[0].bead_radiusnull
fits[0].models[0].lognull
fits[0].models[0].symmetrynull
fits[0].models[0].commentnull
fits[0].models[0].user1074
fits[0].fit_unit1/A
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fits[0].softwareFoXS
fits[0].chi_square_value2.102608
fits[0].p_value0.0
fits[0].fit_residual_plotSASDVG2_fit1_fitfoxsresiduals_img.png
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fits[1].chi_square_value1.273798
fits[1].p_value0.0
fits[1].fit_residual_plotSASDVG2_fit2_fitfoxsresiduals_img.png
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last_modified2024-10-07T14:58:23.076062+02:00
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manifest.json:34 个字段值
字段路径原始值
codeSASDVG2
statussuccess
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查看完整 summary.json 原文
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    "title": "S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.",
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      "title": "S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases.",
      "author_list": "Mishra N, Gido CD, Herdendorf TJ, Hammel M, Hura GL, Fu ZQ, Geisbrecht BV",
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查看完整 manifest.json 原文
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